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  3. Neutral Red

Neutral Red, a nitrogenous pH-indicator with a pKi of 6.8, is an indicator for the internal acidification of thylakoids. Neutral Red stains lysosomes red.

For research use only. We do not sell to patients.

Neutral Red

Neutral Red Chemical Structure

CAS No. : 553-24-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g In-stock
10 g   Get quote  
50 g   Get quote  

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Description

Neutral Red, a nitrogenous pH-indicator with a pKi of 6.8, is an indicator for the internal acidification of thylakoids. Neutral Red stains lysosomes red[1].

In Vitro

As cells begin to die, their ability to incorporate neutral red diminishes. The neutral red is also used to stain cell cultures for plate titration of viruses[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

288.78

Formula

C15H17ClN4

CAS No.
Appearance

Solid

Color

Green to dark green

SMILES

NC1=CC2=NC3=CC(N(C)C)=CC=C3N=C2C=C1C.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, protect from light

In solvent -80°C 1 year
-20°C 6 months
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (173.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4628 mL 17.3142 mL 34.6284 mL
5 mM 0.6926 mL 3.4628 mL 6.9257 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.14%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4628 mL 17.3142 mL 34.6284 mL 86.5711 mL
5 mM 0.6926 mL 3.4628 mL 6.9257 mL 17.3142 mL
10 mM 0.3463 mL 1.7314 mL 3.4628 mL 8.6571 mL
15 mM 0.2309 mL 1.1543 mL 2.3086 mL 5.7714 mL
20 mM 0.1731 mL 0.8657 mL 1.7314 mL 4.3286 mL
25 mM 0.1385 mL 0.6926 mL 1.3851 mL 3.4628 mL
30 mM 0.1154 mL 0.5771 mL 1.1543 mL 2.8857 mL
40 mM 0.0866 mL 0.4329 mL 0.8657 mL 2.1643 mL
50 mM 0.0693 mL 0.3463 mL 0.6926 mL 1.7314 mL
60 mM 0.0577 mL 0.2886 mL 0.5771 mL 1.4429 mL
80 mM 0.0433 mL 0.2164 mL 0.4329 mL 1.0821 mL
100 mM 0.0346 mL 0.1731 mL 0.3463 mL 0.8657 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Neutral Red
Cat. No.:
HY-D0166
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