LPd peroxida probe-1 

LPd peroxida probe-1, a marker of ferroptosis, is a useful fluorescent probe for investigating the roles of lipid peroxidation in a variety of cell pathophysiologies. LPd peroxida probe-1 reduces lipid hydroperoxides to lipid alcohols and is used for imaging lipid hydroperoxides in living cells^[1][2][3].

Guide (The following is our recommended experimental protocol. This protocol is provided as a reference only, and should be adjusted according to your specific needs)^[1].
1. Cells in the culture dish are treated with 50 μM H₂O₂ in culture medium at 37 °C for 2 h. After removing H₂O₂, the cells are washed three times with Hank's Balanced Salt Solution (HBSS).
2. Cells are treated with HBSS containing 10 μM LPd peroxida probe at 37 °C for 30 min. After removing the LPd peroxida probe, the cells are washed three times with HBSS.
3. Fluorescence images are acquired using a fluorescence microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

840.85

C₅₁H₄₁N₂O₈P

1448846-35-2

Solid

Pink to red

535

524

O=C1C2=CC=C3C4=CC=C5C(N(CCOCCOCCOCCOC)C(C6=CC=C(C4=C56)C7=C3C2=C(C(N1C8=CC=C(P(C9=CC=CC=C9)C%10=CC=CC=C%10)C=C8)=O)C=C7)=O)=O

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Kazuo Tomita, et al. MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines. Int J Mol Sci. 2021 Aug 2;22(15):8300.  [Content Brief]

  [2]. Elizabeth M Kenny, et al. Ferroptosis Contributes to Neuronal Death and Functional Outcome After Traumatic Brain Injury. Crit Care Med. 2019 Mar;47(3):410-418.  [Content Brief]

  [3]. Kazunori Yamanaka, et al. A novel fluorescent probe with high sensitivity and selective detection of lipid hydroperoxides in cells. RSC Advances, 2012, 2, 7894–7900.