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  4. Fe2Orange

Fe2Orange is a Fe2+ selective probe with an excitation wavelength of 543 nm and an emission wavelength of 580 nm. Fe2Orange emits fluorescence after binding to intracellular Fe2+, thereby achieving specific labeling of Fe2+. Fe2Orange is used to detect the content and distribution of Fe2+ in cells.

For research use only. We do not sell to patients.

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24 μg In-stock

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Customer Review

Based on 25 publication(s) in Google Scholar

Fe2Orange Related Antibodies

Top Publications Citing Use of Products

    Fe2Orange purchased from MedChemExpress. Usage Cited in: Theranostics. 2025 Aug 16;15(17):9131-9158.  [Abstract]

    FerroOrange (1 μM/mL; 30 min) staining in each treatment group was analyzed by flow cytometry.

    Fe2Orange purchased from MedChemExpress. Usage Cited in: Theranostics. 2025 Aug 16;15(17):9131-9158.  [Abstract]

    Fe2+ ​​levels were detected by FerroOrange staining (1 μM/mL; 30 min).

    Fe2Orange purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Sep 30;44(1):272.  [Abstract]

    FerroOrange (1 μM; 30 min) was used to image LNCaP and C4-2 cells using confocal laser scanning microscopy (CLSM).

    Fe2Orange purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Aug 25.  [Abstract]

    Fe2Orange (1 μM; 30 min). Intracellular chelated iron levels were analyzed in U87 and U251 cells after treatment with 4′-hydroxychalcone for 48 h with or without Fer-1 (25 μM).

    Fe2Orange purchased from MedChemExpress. Usage Cited in: Redox Biol. 2024 Jul:73:103143.

    Results of incubation with JC-1 fluorescent probe, FerroOrange probe, DCFH-DA, and MitoSOX Red are shown.

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    Description

    Fe2Orange is a Fe2+ selective probe with an excitation wavelength of 543 nm and an emission wavelength of 580 nm. Fe2Orange emits fluorescence after binding to intracellular Fe2+, thereby achieving specific labeling of Fe2+. Fe2Orange is used to detect the content and distribution of Fe2+ in cells[1].

    In Vitro

    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Solution preparation
    1.1 Preparation of stock solution
    Solvent: DMSO
    Concentration: 1 mM
    1.2 Preparation of working solution
    Dilute to 1 μM with HBSS or cell culture medium (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately. Keep it away from light.

    2. Cell staining
    2.1 Cells are plated in 96-well microplates.
    2.2 Discard the cell culture medium and wash the cells three times with HBSS or serum-free medium.
    2.3 Add the 1 μM FerroOrange working solution and incubate for 30 minutes at 37°C
    2.4 Using fluorescence microscopy at Ex/Em = 540/585 nm.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Fe2Orange Related Antibodies
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    [Fe2Orange]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Purity & Documentation

    Purity: 98.2%

    SDS (251 KB)

    COA (243 KB)

    Handling Instructions (2659 KB)
    References
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    Fe2Orange Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Fe2OrangeFluorescent DyeHBSSHT-22FeInhibitorinhibitorinhibit

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