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  3. Ferroptosis Environmental Pollutants
  4. Ammonium iron(III) citrate

Ammonium iron(III) citrate  (Synonyms: Ammonium ferric citrate; FAC)

Cat. No.: HY-B1645 Purity: 98.0%
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Ammonium iron(III) citrate (Ammonium ferric citrate), a physiological form of nonetransferrin-bound iron, induces intracellular iron overload to cause ferroptosis. Ammonium iron(III) citrate can enhance protein production.

For research use only. We do not sell to patients.

CAS No. : 1185-57-5

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Customer Review

Based on 28 publication(s) in Google Scholar

Other Forms of Ammonium iron(III) citrate:

Ammonium iron(III) citrate Related Antibodies

Top Publications Citing Use of Products
Cell Proliferation/Viability Assay
WB
Bio/Physico-chemical Assay
Cell Imaging/Staining

    Ammonium iron(III) citrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 7:e18323.  [Abstract]

    Immunoblot analysis of FDX1 and mitochondrial marker TOMM20 in OSRC2 cells treated for 24 h with either FAC (Ammonium iron(III) citrate) 0–4 μM.

    Ammonium iron(III) citrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 21:e2505359.  [Abstract]

    Ferroptosis was induced by erastin or FAC (Ammonium iron(III) citrate; 0-1600 μM) with different doses, and cell viability was analyzed by the CCK-8 after cells were treated for 2 days.

    Ammonium iron(III) citrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 21:e2505359.  [Abstract]

    Intracellular ROS was assessed using the DCFH-DA probe after being treated with 30 µM erastin or 500 µM FAC (Ammonium iron(III) citrate) for 4 h.

    Ammonium iron(III) citrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 21:e2505359.  [Abstract]

    Intracellular Fe2+ levels under 30 µM erastin or 500 µM FAC (Ammonium iron(III) citrate) treatment for 4 h were detected with the RhoNox-1(1μM; 1 h) fluorescent probe.

    Ammonium iron(III) citrate purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 21:e2505359.  [Abstract]

    Representative images of ferroptotic cells among iPSC-RPE cells, De-iPSC-RPE cells, and iRPE cells treated with 30 µM erastin or 500 µM FAC (Ammonium iron(III) citrate) for 2 days.

    • Biological Activity

    • Purity & Documentation

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    • Customer Review

    Description

    Ammonium iron(III) citrate (Ammonium ferric citrate), a physiological form of nonetransferrin-bound iron, induces intracellular iron overload to cause ferroptosis[1]. Ammonium iron(III) citrate can enhance protein production[2].

    In Vitro

    Ammonium iron(III) citrate (Ammonium ferric citrate; 1, 5, 10, 15 mM; 24 hours) with 5 mM induces cell death in HT1080 cells. AML12 cells were highly resistant to Ammonium iron(III) citrate, as they maintained cell viability about 80%. Ammonium iron(III) citrate can be actively transported into cells by specific transporter[1].
    Ammonium iron(III) citrate (6, 12, 18 mg/L) has a higher biomas for Chlorella vulgaris FSP-E (CV) in BG-11 medium with 12 mg/L and 18 mg/L[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ammonium iron(III) citrate Related Antibodies
    Clinical Trial
    Formula

    C6H8O7.xFe.xH3N
    CAS No.
    Appearance

    Solid

    Color

    Light brown to brown

    SMILES

    OC(CC(C(O)=O)(O)CC(O)=O)=O.[x Fe(III)].[x NH3]
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Store at room temperature, keep dry and cool

    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    H2O : 33.33 mg/mL (Need ultrasonic)

    DMSO : < 1 mg/mL (insoluble or slightly soluble)

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 50 mg/mL; Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.0%

    SDS (393 KB)

    COA (253 KB) IR (143 KB)

    Handling Instructions (2659 KB)
    References
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    Ammonium iron(III) citrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ammonium iron(III)1185-57-5Ammonium ferric FACFerroptosisEnvironmental PollutantsEnvironmental ContaminantsHT1080AML12ironInhibitorinhibitorinhibit

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