2. Apoptosis Anti-infection
  3. Ferroptosis Fungal
  4. Ferrostatin-1

Ferrostatin-1  (Synonyms: Fer-1)

Cat. No.: HY-100579 Purity: 99.96%
COA Handling Instructions

Ferrostatin-1 (Fer-1), a potent and selective ferroptosis inhibitor, suppresses Erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1, a synthetic antioxidant, acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death. Ferrostatin-1 exhibits antifungal activity.

For research use only. We do not sell to patients.

Ferrostatin-1 Chemical Structure

Ferrostatin-1 Chemical Structure

CAS No. : 347174-05-4

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 79 In-stock
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10 mg USD 108 In-stock
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Customer Review

Based on 452 publication(s) in Google Scholar

Ferrostatin-1 Related Antibodies

Top Publications Citing Use of Products

439 Publications Citing Use of MCE Ferrostatin-1

WB
IF
Proliferation Assay
Cell Viability Assay

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2023 May.

    Ferrostatin-1 (Fer-1) significantly restores the decreased cell viability in HK-1 and 5-8F cells induced by α-solanine.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Sleep Breath. 2023 Mar 1.  [Abstract]

    The protein content of GPX4 and FTH1 is signifcantly upregulated in intermittent hypoxia (IH)+ferrostatin-1 (5 μM) group, in rat liver (BRL‑3A) cells.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    U2OS cells are incubated with DMSO or OSMI-1 for 12 h and then pre-treated with Ferrostatin-1 (Fer-1; 2 μM) for 2 h before the treatment of RSL3 for 4 h. Cells are stained with PI and cell death is then assessed by flow cytometry.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Oct;56:102435.  [Abstract]

    Primary HSCs and LX-2 cells are treated with EA (LX-2 cells: 40 μM; primary HSCs: 45 μM), Ferrostatin-1 (1 μM) and ZVAD-FMK (10 μM), and cells are stained with PI (red fluorescence) to examine the dead cells.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022 Feb;76:103847.  [Abstract]

    Mitochondrial membrane potential (ΔΨm) measured by fluorescence microscopy of MCC treated with TBHP and rescued via Ferrostatin-1 (Fer-1; 1 μM) using JC-1 Mitochondrial Membrane Potential Assay. In Fer-1 and DFO group, red fluorescence (poly JC-1) is increased and green fluorescence (mono JC-1) is decreased.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2021 Nov 19;13:23-36.  [Abstract]

    H1299 and H460 cells are treated with Curcumenol with or without Ferrostatin-1 (Fer-1; 1 μM) for 24 h, and the cell viability is analyzed.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2021 Jun 23;40(1):206.  [Abstract]

    T47D cells are treated with Metformin (5 mM) for 48 h in the absence or presence of Ferrostatin-1 (1 μM) for 48 h.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Jul 10;510:93-104.  [Abstract]

    Ferrostatin-1(2 μM) has the ability to fully restore the cell viability impaired by Erastin (5 μM) treated control cells in GBC-SD cells.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Mar 1;17(4):942-956.  [Abstract]

    After the gallic acid treatment, Fe2+, ROS, and MDA are significantly increased compared to the control group, and at the same time, the content of GSH is significantly reduced. The intervention of Ferrostatin-1 (2 μM) while treating the cells with gallic acid reversed the changes in these indicators to varying degrees.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Mar 1;17(4):942-956.  [Abstract]

    Gallic acid affects the expression of ferroptosis-related proteins. The relative expression levels of GPX4, SLC7A11, and SIGMAR1 decreased, while the relative expression levels of ATF4 and TFR1 increased. Ferrostatin-1 (2 μM) as a ferroptosis inhibitor reverses the expression trend of these proteins.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2020 May 8;5(1):51.  [Abstract]

    Erastin-induced cell death in lung cancer cells is almost blocked by cotreatment with the Ferrostatin-1 (Fer-1; 24 h) in H460 and H1299 cells.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Aug 2;10(21):9865-9887.  [Abstract]

    p53 expression of HepG2 cells after treated with Fer-1 at a concentration of 0-40 nM. The addition of Fer-1 could repress the expression of p53 protein by inhibiting the ferroptosis of SFB formulation.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Sep 15;11(9):756.  [Abstract]

    Acot1 protein levels in different concentrations of DOX-treated HL-1 cell with or without Fer-1 (10 μM) for 24 h. Acot1 expression is decreased at a higher concentration of DOX, while Fer-1 co-treatment partially suppressed this trend.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ferrostatin-1 (Fer-1), a potent and selective ferroptosis inhibitor, suppresses Erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1, a synthetic antioxidant, acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death. Ferrostatin-1 exhibits antifungal activity[1][2][3].

    IC50 & Target

    EC50: 60 nM (Ferroptosis)[1]
    In Vitro

    Ferrostatin-1 prevents erastin-induced accumulation of cytosolic and lipid ROS. Ferrostatin-1 prevents glutamate-induced neurotoxicity in organotypic rat brain slices[1].
    Ferrostatin-1 (2 μM; 24 h) prevents Glutamate (5 mM)-induced neurotoxicity in a rat organotypic hippocampal slice culture (OHSC)[2].
    Ferrostatin-1 inhibits lipid peroxidation, but not mitochondrial reactive oxygen species formation or lysosomal membrane permeability[2].
    Ferrostatin-1 inhibits cell death in cellular models of Huntington's disease (HD), periventricular leukomalacia (PVL), and kidney dysfunction[2].
    Ferrostatin-1 (1 μM; 6 h) inhibits the oxidative destruction of unsaturated fatty acids in HT-1080 cells, thus increases the number of healthy medium spiny neurons (MSNs)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ferrostatin-1 Related Antibodies
    In Vivo

    Ferrostatin-1 (5 mg/kg; ip; single dose, 30 min before glycerol injection) improves renal function in mice with rhabdomyolysis, whereas no beneficial effects were observed with the pan-caspase inhibitor zVAD or in RIPK3-deficient mice[1].
    Ferrostatin-1 (0.8 mg/kg; tail vein injection) effectively alleviates LPS-induced induced acute lung injury (ALI)[4].
    Ferrostatin-1 (i.p.; 5 mg/kg; C57BL/6J mice) improves renal function in mice with rhabdomyolysis[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57BL/6 mice (LPS-induced ALI)[4]
    Dosage: 0.8 mg/kg
    Administration: Tail vein injection
    Result: Exerted therapeutic action against LPS-induced ALI.
    Molecular Weight

    262.35

    Appearance

    Solid

    Formula

    C15H22N2O2
    CAS No.
    SMILES

    O=C(OCC)C1=CC=C(NC2CCCCC2)C(N)=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (476.46 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.8117 mL 19.0585 mL 38.1170 mL
    5 mM 0.7623 mL 3.8117 mL 7.6234 mL
    10 mM 0.3812 mL 1.9059 mL 3.8117 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.53 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: 2.5 mg/mL (9.53 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.93 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% Saline

      Solubility: 0.2 mg/mL (0.76 mM); Clear solution; Need ultrasonic

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.96%

    COA (245 KB) HNMR (269 KB) LCMS (267 KB) Elemental Analysis Report (104 KB)

    Handling Instructions (2659 KB)
    References
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    Ferrostatin-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    Ferrostatin-1347174-05-4Fer-1Ferrostatin1Ferrostatin 1Fer1Fer 1FerroptosisFungalantioxidantantifungalcelldeathcytosolicROSneurotoxicityInhibitorinhibitorinhibit

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