1. Apoptosis Anti-infection
  2. Ferroptosis Fungal
  3. Ferrostatin-1

Ferrostatin-1  (Synonyms: Fer-1)

Cat. No.: HY-100579 Purity: 99.96%
COA Handling Instructions

Ferrostatin-1 (Fer-1), a potent and selective ferroptosis inhibitor, suppresses Erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1, a synthetic antioxidant, acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death. Ferrostatin-1 exhibits antifungal activity.

For research use only. We do not sell to patients.

Ferrostatin-1 Chemical Structure

Ferrostatin-1 Chemical Structure

CAS No. : 347174-05-4

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 79 In-stock
Solution
10 mM * 1 mL in DMSO USD 79 In-stock
Solid
5 mg USD 72 In-stock
10 mg USD 108 In-stock
25 mg USD 228 In-stock
50 mg USD 396 In-stock
100 mg USD 580 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 515 publication(s) in Google Scholar

Top Publications Citing Use of Products

502 Publications Citing Use of MCE Ferrostatin-1

WB
IF
Proliferation Assay
Cell Viability Assay

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2023 May.

    Ferrostatin-1 (Fer-1) significantly restores the decreased cell viability in HK-1 and 5-8F cells induced by α-solanine.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Sleep Breath. 2023 Mar 1.  [Abstract]

    The protein content of GPX4 and FTH1 is signifcantly upregulated in intermittent hypoxia (IH)+ferrostatin-1 (5 μM) group, in rat liver (BRL‑3A) cells.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    U2OS cells are incubated with DMSO or OSMI-1 for 12 h and then pre-treated with Ferrostatin-1 (Fer-1; 2 μM) for 2 h before the treatment of RSL3 for 4 h. Cells are stained with PI and cell death is then assessed by flow cytometry.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Oct;56:102435.  [Abstract]

    Primary HSCs and LX-2 cells are treated with EA (LX-2 cells: 40 μM; primary HSCs: 45 μM), Ferrostatin-1 (1 μM) and ZVAD-FMK (10 μM), and cells are stained with PI (red fluorescence) to examine the dead cells.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: EBioMedicine. 2022 Feb;76:103847.  [Abstract]

    Mitochondrial membrane potential (ΔΨm) measured by fluorescence microscopy of MCC treated with TBHP and rescued via Ferrostatin-1 (Fer-1; 1 μM) using JC-1 Mitochondrial Membrane Potential Assay. In Fer-1 and DFO group, red fluorescence (poly JC-1) is increased and green fluorescence (mono JC-1) is decreased.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2021 Nov 19;13:23-36.  [Abstract]

    H1299 and H460 cells are treated with Curcumenol with or without Ferrostatin-1 (Fer-1; 1 μM) for 24 h, and the cell viability is analyzed.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2021 Jun 23;40(1):206.  [Abstract]

    T47D cells are treated with Metformin (5 mM) for 48 h in the absence or presence of Ferrostatin-1 (1 μM) for 48 h.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Jul 10;510:93-104.  [Abstract]

    Ferrostatin-1(2 μM) has the ability to fully restore the cell viability impaired by Erastin (5 μM) treated control cells in GBC-SD cells.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Mar 1;17(4):942-956.  [Abstract]

    After the gallic acid treatment, Fe2+, ROS, and MDA are significantly increased compared to the control group, and at the same time, the content of GSH is significantly reduced. The intervention of Ferrostatin-1 (2 μM) while treating the cells with gallic acid reversed the changes in these indicators to varying degrees.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2021 Mar 1;17(4):942-956.  [Abstract]

    Gallic acid affects the expression of ferroptosis-related proteins. The relative expression levels of GPX4, SLC7A11, and SIGMAR1 decreased, while the relative expression levels of ATF4 and TFR1 increased. Ferrostatin-1 (2 μM) as a ferroptosis inhibitor reverses the expression trend of these proteins.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2020 May 8;5(1):51.  [Abstract]

    Erastin-induced cell death in lung cancer cells is almost blocked by cotreatment with the Ferrostatin-1 (Fer-1; 24 h) in H460 and H1299 cells.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Theranostics. 2020 Aug 2;10(21):9865-9887.  [Abstract]

    p53 expression of HepG2 cells after treated with Fer-1 at a concentration of 0-40 nM. The addition of Fer-1 could repress the expression of p53 protein by inhibiting the ferroptosis of SFB formulation.

    Ferrostatin-1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Sep 15;11(9):756.  [Abstract]

    Acot1 protein levels in different concentrations of DOX-treated HL-1 cell with or without Fer-1 (10 μM) for 24 h. Acot1 expression is decreased at a higher concentration of DOX, while Fer-1 co-treatment partially suppressed this trend.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ferrostatin-1 (Fer-1), a potent and selective ferroptosis inhibitor, suppresses Erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1, a synthetic antioxidant, acts via a reductive mechanism to prevent damage to membrane lipids and thereby inhibits cell death. Ferrostatin-1 exhibits antifungal activity[1][2][3].

    IC50 & Target

    EC50: 60 nM (Ferroptosis)[1]

    In Vitro

    Ferrostatin-1 prevents erastin-induced accumulation of cytosolic and lipid ROS. Ferrostatin-1 prevents glutamate-induced neurotoxicity in organotypic rat brain slices[1].
    Ferrostatin-1 (2 μM; 24 h) prevents Glutamate (5 mM)-induced neurotoxicity in a rat organotypic hippocampal slice culture (OHSC)[2].
    Ferrostatin-1 inhibits lipid peroxidation, but not mitochondrial reactive oxygen species formation or lysosomal membrane permeability[2].
    Ferrostatin-1 inhibits cell death in cellular models of Huntington's disease (HD), periventricular leukomalacia (PVL), and kidney dysfunction[2].
    Ferrostatin-1 (1 μM; 6 h) inhibits the oxidative destruction of unsaturated fatty acids in HT-1080 cells, thus increases the number of healthy medium spiny neurons (MSNs)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Ferrostatin-1 (5 mg/kg; ip; single dose, 30 min before glycerol injection) improves renal function in mice with rhabdomyolysis, whereas no beneficial effects were observed with the pan-caspase inhibitor zVAD or in RIPK3-deficient mice[1].
    Ferrostatin-1 (0.8 mg/kg; tail vein injection) effectively alleviates LPS-induced induced acute lung injury (ALI)[4].
    Ferrostatin-1 (i.p.; 5 mg/kg; C57BL/6J mice) improves renal function in mice with rhabdomyolysis[5].
    Ferrostatin-1 (10 mg/kg/d, i.p., 3 d) attenuates hypoxic-ischemic brain damage-, oxygen-glucose deprivation-, or Erastin (HY-15763)-induced ferroptosis in brain of neonatal rats[6].
    Ferrostatin-1 (0.655 mg/kg, i.p., 3 times a week for 6 week) exerts anti-ferroptosis effects by increasing GPX4 activity and by inhibiting lipid peroxidation in the salivary gland of ovariectomized (postmenopausal model) rats[7].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male C57BL/6 mice (LPS-induced ALI)[4]
    Dosage: 0.8 mg/kg
    Administration: Tail vein injection
    Result: Exerted therapeutic action against LPS-induced ALI.
    Molecular Weight

    262.35

    Formula

    C15H22N2O2

    CAS No.
    Appearance

    Solid

    SMILES

    O=C(OCC)C1=CC=C(NC2CCCCC2)C(N)=C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 125 mg/mL (476.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.8117 mL 19.0585 mL 38.1170 mL
    5 mM 0.7623 mL 3.8117 mL 7.6234 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (9.53 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: 2.5 mg/mL (9.53 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.96%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.8117 mL 19.0585 mL 38.1170 mL 95.2925 mL
    5 mM 0.7623 mL 3.8117 mL 7.6234 mL 19.0585 mL
    10 mM 0.3812 mL 1.9059 mL 3.8117 mL 9.5293 mL
    15 mM 0.2541 mL 1.2706 mL 2.5411 mL 6.3528 mL
    20 mM 0.1906 mL 0.9529 mL 1.9059 mL 4.7646 mL
    25 mM 0.1525 mL 0.7623 mL 1.5247 mL 3.8117 mL
    30 mM 0.1271 mL 0.6353 mL 1.2706 mL 3.1764 mL
    40 mM 0.0953 mL 0.4765 mL 0.9529 mL 2.3823 mL
    50 mM 0.0762 mL 0.3812 mL 0.7623 mL 1.9059 mL
    60 mM 0.0635 mL 0.3176 mL 0.6353 mL 1.5882 mL
    80 mM 0.0476 mL 0.2382 mL 0.4765 mL 1.1912 mL
    100 mM 0.0381 mL 0.1906 mL 0.3812 mL 0.9529 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Ferrostatin-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

     

    Salutation

    Applicant Name *

     

    Email Address *

    Phone Number *

     

    Organization Name *

    Department *

     

    Requested quantity *

    Country or Region *

         

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Ferrostatin-1
    Cat. No.:
    HY-100579
    Quantity:
    MCE Japan Authorized Agent: