1. Apoptosis
  2. Ferroptosis
  3. Ferrostatin-1

Ferrostatin-1 

Cat. No.: HY-100579 Purity: 99.56%
Handling Instructions

Ferrostatin-1 is a potent inhibitor of ferroptosis with an EC50 of 60 nM.

For research use only. We do not sell to patients.

Ferrostatin-1 Chemical Structure

Ferrostatin-1 Chemical Structure

CAS No. : 347174-05-4

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10 mM * 1  mL in DMSO USD 79 In-stock
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Customer Review

Based on 25 publication(s) in Google Scholar

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Description

Ferrostatin-1 is a potent inhibitor of ferroptosis with an EC50 of 60 nM.

IC50 & Target

EC50: 60 nM (Ferroptosis)[1]

In Vitro

Ferrostatin-1 is the most potent inhibitor of erastin-induced ferroptosis in HT-1080 cells (EC50=60 nM). Ferrostatin-1 does not inhibit ERK phosphorylation or arrest the proliferation of HT-1080 cells. Ferrostatin-1 does, however, prevent erastin-induced accumulation of cytosolic and lipid ROS[1]. Cells pretreated with 0.4 μM Ferrostatin-1 displays significantly reduce intracellular reactive oxygen species (ROS) and nitrogen species (RNS) below basal levels. Additionally, increased intracellular ROS levels are also significantly lowered below basal levels by a 0.4 µM Ferrostatin-1 pretreatment. Ferrostatin-1 treatment for 24 h does not change the expression level of i-NOS in SHSY-5Y cell when compare with vehicle (0.02 % DMSO) treated cells[2].

Molecular Weight

262.35

Formula

C₁₅H₂₂N₂O₂

CAS No.

347174-05-4

SMILES

O=C(OCC)C1=CC=C(NC2CCCCC2)C(N)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 150 mg/mL (571.76 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8117 mL 19.0585 mL 38.1170 mL
5 mM 0.7623 mL 3.8117 mL 7.6234 mL
10 mM 0.3812 mL 1.9059 mL 3.8117 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (9.53 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (9.53 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[2]

The cells treated for 24 h with Ferrostatin-1 are washed three times with phosphate-buffered saline (PBS) and pelleted by centrifugation. The supernatant is removed and 80 μL of lysis buffer is added to the cells and then stored overnight at -20°C. Subsequently, the cells are centrifuged at 10,000 RPM for 12 min and both the pellet and supernatant are stored for future use[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[2]

SH-S5Y cells are seeded in 24-well plates. After overnight incubation, the cells are exposed for 24 h to 1 µM of Ferrostatin-1, under standard growth conditions. Bright field photomicrographs are captured using an inverted microscope equipped with 10× objective in a live-cell modality[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.56%

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Keywords:

Ferrostatin-1Ferrostatin1Ferrostatin 1FerroptosisInhibitorinhibitorinhibit

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Ferrostatin-1
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HY-100579
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