5-ALA-PDT induced ferroptosis in keloid fibroblasts via ROS, accompanied by downregulation of xCT, GPX4

  • Photodiagnosis Photodyn Ther. 2023 May 21;103612. doi: 10.1016/j.pdpdt.2023.103612.
Jiheng Zhang  1 ,  Lulu Liu  1 ,  Xinying Li  1 ,  Xiaoxiao Shen  2 ,  Guihong Yang  3 ,  Yumeng Deng  4 ,  Zhengwei Hu  5 ,  Junbo Zhang  6 ,  Yuangang Lu  7
Affiliations
  • 1. Department of Plastic and Cosmetic Surgery, Daping Hospital, Army Medical University, Chongqing, China.
  • 2. Bioengineering College of Chongqing University, Chongqing, China.
  • 3. Department of Dermatology, Daping Hospital, Army Medical University, Chongqing, China.
  • 4. Department of Dermatology, Third Affiliated Hospital of Chongqing Medical University, Chongqing, China.
  • 5. Department of Urology, Daping Hospital, Army Medical University, Chongqing, China.
  • 6. Department of Plastic and Cosmetic Surgery, Daping Hospital, Army Medical University, Chongqing, China. Electronic address: [email protected].
  • 7. Department of Plastic and Cosmetic Surgery, Daping Hospital, Army Medical University, Chongqing, China. Electronic address: [email protected].
PMID: 37220842 DOI: 10.1016/j.pdpdt.2023.103612
Abstract

Keloids display many cancerous properties, including uncontrolled and invasive growth, high rates of recurrence as well as similar bioenergetics. 5-aminolevulinic acid-based photodynamic therapy (5-ALA-PDT) is an effective treatment that performs cytotoxic effects by producing Reactive Oxygen Species (ROS), which is linked to lipid peroxidation and Ferroptosis. Herein, we explored underlying mechanisms of 5-ALA-PDT against keloids. We identified that 5-ALA-PDT led to elevated levels of ROS and lipid peroxidation in keloid fibroblasts, accompanied by downregulation of xCT and GPX4, which are associated with anti-oxidation effects and Ferroptosis inhibition. These results may indicate that 5-ALA-PDT treatment increases ROS while inhibiting xCT and GPX4, thus promoting lipid peroxidation to induce Ferroptosis in keloid fibroblasts.

Keywords
PDT; ferroptosis; fibroblast; keloids.
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