Pleurotus citrinopileatus polysaccharides ameliorate DSS-induced ulcerative colitis by improving intestinal barrier and inflammation modulation via NF-κB pathway

  • Int J Biol Macromol. 2025 Sep 4;327(Pt 2):147256. doi: 10.1016/j.ijbiomac.2025.147256.
Chao Li  1 ,  Lu Zhao  2 ,  Shichao Yu  2 ,  Xin Jiang  3 ,  Fang Xie  2 ,  Cheng Ma  4 ,  Zhaoli Meng  5
Affiliations
  • 1. Department of Neurology, Stroke Center, The First Hospital of Jilin University, Changchun, 130021, China.
  • 2. Laboratory of Tumor Immunology, The first Hospital of Jilin University, Changchun, 130021, China.
  • 3. Department of Biochemistry, College of Basic Medical Sciences, Jilin University, Changchun, 130021, China.
  • 4. Jilin Yatai Biopharmaceutical Co., Ltd, Changchun, 130032, China.
  • 5. Laboratory of Tumor Immunology, The first Hospital of Jilin University, Changchun, 130021, China. Electronic address: [email protected].
PMID: 40914366 DOI: 10.1016/j.ijbiomac.2025.147256
Abstract

The aim was to investigate the pharmacological effects of a polysaccharide isolated from Pleurotus citrinopileatus on ulcerative colitis (UC). One polysaccharide, CP-2-2, was isolated and purified from P. citrinopileatus body fruit using chromatographic methods. CP-2-2 consisted of Fuc, Ara, Gal, Man, and Fru, with molar ratios of 1.38 %, 7.54 %, 84.49 %, 5.86 %, and 0.73 %, respectively, with Mw of 32.5 kDa, and its FITR and methylation were analyzed. In dextran sulfate sodium(DSS)-induced UC mice, CP-2-2 significantly alleviated UC injuries including decreasing body weight loss and disease activity index (DAI), and increasing colon length. We also found CP-2-2 significantly inhibited the production of inflammatory cytokines. Immunofluorescence staining and western blotting results showed CP-2-2 might regulate inflammation, partly through the NF-κB pathway. Furthermore, CP-2-2 treatment significantly ameliorated intestinal pathological damage and improved the intestinal barrier. In the in vitro experiments, we found that CP-2-2 was involved in the inhibition of Apoptosis and Ferroptosis. Our results revealed the protective effects of CP-2-2 against DSS-induced colitis could be partly attributed to its inhibitory role in NF-κB signaling and its protective effects on intestinal epithelial cells, suggesting CP-2-2 might be a potential functional agent.

Keywords
Intestinal barrier; NF-κB; Pleurotus citrinopileatus polysaccharides; Ulcerative colitis.
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