2. Apoptosis
  3. Caspase
  4. Ac-DEVD-CHO

Ac-DEVD-CHO 

Cat. No.: HY-P1001 Purity: 98.66%
COA Handling Instructions

Ac-DEVD-CHO is a specific Caspase-3 inhibitor with a Ki value of 230 pM.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-DEVD-CHO Chemical Structure

Ac-DEVD-CHO Chemical Structure

CAS No. : 169332-60-9

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1 mg USD 84 In-stock
Estimated Time of Arrival: December 31
5 mg USD 288 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 22 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Ac-DEVD-CHO purchased from MCE. Usage Cited in: Apoptosis. 2019 Oct;24(9-10):703-717.  [Abstract]

    BMDMs were first primed with LPS (500 ng/ml) for 4 h, pre-treated with or without MCC950 (1 μM) and caspase-3 inhibitor Ac-DEVD-CHO (DEVD) for 1 h and followed by stimulation with ATP (5 mM) in the presence or absence of MCC950. The cell lysates are collected and western blotting is performed to assess the expression levels of indicated proteins.

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    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ac-DEVD-CHO is a specific Caspase-3 inhibitor with a Ki value of 230 pM.

    IC50 & Target[1]

    Caspase 3

    0.23 nM (Ki)

    Caspase-8

    0.92 nM (Ki)

    Caspase-7

    1.6 nM (Ki)

    Caspase-10

    12 nM (Ki)

    Caspase-1

    18 nM (Ki)

    Caspase-6

    31 nM (Ki)

    Caspase-9

    60 nM (Ki)

    Caspase-4

    132 nM (Ki)

    Caspase-5

    205 nM (Ki)

    Caspase-2

    1710 nM (Ki)

    In Vitro

    To ascertain the role of caspase-3 in SLNT-induced apoptosis, a caspase-3 inhibitor (Ac-DEVD-CHO) is used. The addition of Ac-DEVD-CHO significantly prevents SLNT-induced apoptosis (from 32.91±1.21% decreases to 15.88±1.58% while NC and Ac-DEVD-CHO groups are 6.45±0.96%, 7.77±0.79%, respectively)[2]. The apoptosis rates of cells pretreated with zVAD-fmk (5.32%) or Ac-DEVD-CHO (7.43%) decrease obviously after hypericin-mediated PDT treatment[3]. Remarkably, 10 μmol/L Ac-DEVD-CHO partially blocks the effect of SIN-induced apoptosis and reduces the number of apoptotic nuclei. These effects of SIN are blocked by the caspase-3 inhibitor Ac-DEVD-CHO. Camptothecin (4 μM), a positive control, increases caspase-3 activity, which is also blocked by Ac-DEVD-CHO[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    In Vivo

    Compare with model group, in CI group, the concentrations of serum BUN are decreased significantly at all time points after operation and those of Cr are decreased significantly at 6 hours, then restored to those of the sham group at 12 hours and 24 hours; the concentrations of serum TNF-α, IL-6 are decreased and those of IL-10 are elevated significantly at all time points. [TNF-α (μg/L) 6 hours: 436.2±64.2 vs. 653.6±8.9, 12 hours: 233.4±85.4 vs. 579.7±137.1, 24 hours: 151.0±90.3 vs. 551.0±119.8, IL-6 (μg/L) 6 hours: 1033.2±345.8 vs. 1 595.3±159.4, 12 hours: 366.3±68.3 vs. 1 330.7±249.8, 24 hours: 241.2±208.4 vs. 815.3±572.7, IL-10 (μg/L) 6 hours: 33.6±10.4 vs. 26.6±4.5, 12 hours: 37.2±5.0 vs. 24.5±4.3, 24 hours: 38.3±5.5 vs. 18.2±1.6, all P<0.05]; the renal cell apoptosis rates are decreased significantly at all time points: apoptosis rates 6 hours: (13.9±3.2)% vs. (18.3±1.4)%, 12 hours: (10.5±3.6)% vs. (15.9±3.5)%, 24 hours: (8.4±1.8)% vs.(12.5±2.1)%[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    502.47

    Appearance

    Solid

    Formula

    C20H30N4O11
    CAS No.
    Sequence

    N-Acetyl-Asp-Glu-Val-Asp-al
    Sequence Shortening

    Ac-DEVD-al
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 50 mg/mL (99.51 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9902 mL 9.9508 mL 19.9017 mL
    5 mM 0.3980 mL 1.9902 mL 3.9803 mL
    10 mM 0.1990 mL 0.9951 mL 1.9902 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (199.02 mM); Clear solution; Need ultrasonic

    *All of the co-solvents are available by MCE.
    Purity & Documentation
    References
    Cell Assay
    [4]

    OCLs are incubated with RANKL and treated with 0.5 mM SIN with or without the specific caspase-3 inhibitor Ac-DEVD-CHO (10 μM) for 24 h. At the end of the treatment, the cells are washed with PBS and are stained for 15 min with 10 μM Hoechst 33258 dye. Images of the staineing cells are captured with a fluorescent microscope. The differences are evaluated by counting the number of cells with apoptotic nuclear condensation in each well[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [5]

    One hundred and two male mice are subjected to cecal ligation and puncture or sham operation. The animals are assigned into three equal groups (n=34) according to random number table: sham group, model group, and caspase-3 inhibitor (CI) group. Thirty minutes before CLP, Ac-DEVD-CHO (4 μg/g) is injected subcutaneously in CI group. The levels of blood urea nitrogen (BUN) and creatinine (Cr) are determined, and the concentrations of tumor necrosis factor-α (TNF-α), interleukins (IL-6 and IL-10) are measured by enzyme linked immunosorbent assay (ELISA), the renal cell apoptosis rate is determined by flow cytometry. The 4-day and 7-day survival rates of three groups of mice are observed[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Keywords:

    Ac-DEVD-CHO169332-60-9CaspaseInhibitorinhibitorinhibit

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