1. Apoptosis
  2. Caspase
  3. Ac-DEVD-CHO

Ac-DEVD-CHO is a specific Caspase-3 inhibitor with a Ki value of 230 pM.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-DEVD-CHO Chemical Structure

Ac-DEVD-CHO Chemical Structure

CAS No. : 169332-60-9

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1 mg USD 180 In-stock
5 mg USD 550 In-stock
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Customer Review

Based on 28 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Ac-DEVD-CHO purchased from MedChemExpress. Usage Cited in: Apoptosis. 2019 Oct;24(9-10):703-717.  [Abstract]

    BMDMs were first primed with LPS (500 ng/ml) for 4 h, pre-treated with or without MCC950 (1 μM) and caspase-3 inhibitor Ac-DEVD-CHO (DEVD) for 1 h and followed by stimulation with ATP (5 mM) in the presence or absence of MCC950. The cell lysates are collected and western blotting is performed to assess the expression levels of indicated proteins.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ac-DEVD-CHO is a specific Caspase-3 inhibitor with a Ki value of 230 pM.

    IC50 & Target[1]

    Caspase 3

    0.23 nM (Ki)

    Caspase-8

    0.92 nM (Ki)

    Caspase-7

    1.6 nM (Ki)

    Caspase-10

    12 nM (Ki)

    Caspase-1

    18 nM (Ki)

    Caspase-6

    31 nM (Ki)

    Caspase-9

    60 nM (Ki)

    Caspase-4

    132 nM (Ki)

    Caspase-5

    205 nM (Ki)

    Caspase-2

    1710 nM (Ki)

    In Vitro

    To ascertain the role of caspase-3 in SLNT-induced apoptosis, a? caspase-3 inhibitor (Ac-DEVD-CHO) is used. The addition of Ac-DEVD-CHO significantly prevents SLNT-induced apoptosis (from 32.91±1.21% decreases to 15.88±1.58% while NC and Ac-DEVD-CHO groups are 6.45±0.96%, 7.77±0.79%, respectively)[2].
    The apoptosis rates of cells pretreated with zVAD-fmk (5.32%) or Ac-DEVD-CHO (7.43%) decrease obviously after hypericin-mediated PDT treatment[3].
    Remarkably, 10 μmol/L Ac-DEVD-CHO partially blocks the effect of SIN-induced apoptosis and reduces the number of apoptotic nuclei. These effects of SIN are blocked by the caspase-3 inhibitor Ac-DEVD-CHO. Camptothecin (4 μM), a positive control, increases caspase-3 activity, which is also blocked by Ac-DEVD-CHO[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Compare with model group, in CI group, the concentrations of serum BUN are decreased significantly at all time points after operation and those of Cr are decreased significantly at 6 hours, then restored to those of the sham group at 12 hours and 24 hours; the concentrations of serum TNF-α, IL-6 are decreased and those of IL-10 are elevated significantly at all time points. [TNF-α (μg/L) 6 hours: 436.2±64.2 vs. 653.6±8.9, 12 hours: 233.4±85.4 vs. 579.7±137.1, 24 hours: 151.0±90.3 vs. 551.0±119.8, IL-6 (μg/L) 6 hours: 1033.2±345.8 vs. 1 595.3±159.4, 12 hours: 366.3±68.3 vs. 1 330.7±249.8, 24 hours: 241.2±208.4 vs. 815.3±572.7, IL-10 (μg/L) 6 hours: 33.6±10.4 vs. 26.6±4.5, 12 hours: 37.2±5.0 vs. 24.5±4.3, 24 hours: 38.3±5.5 vs. 18.2±1.6, all P<0.05]; the renal cell apoptosis rates are decreased significantly at all time points: apoptosis rates 6 hours: (13.9±3.2)% vs. (18.3±1.4)%, 12 hours: (10.5±3.6)% vs. (15.9±3.5)%, 24 hours: (8.4±1.8)% vs.(12.5±2.1)%[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    502.47

    Formula

    C20H30N4O11

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    N-Acetyl-Asp-Glu-Val-Asp-al

    Sequence Shortening

    Ac-DEVD-al

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Sealed storage, away from moisture and light, under nitrogen

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 50 mg/mL (99.51 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9902 mL 9.9508 mL 19.9017 mL
    5 mM 0.3980 mL 1.9902 mL 3.9803 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (199.02 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.94%

    References
    Cell Assay
    [4]

    OCLs are incubated with RANKL and treated with 0.5 mM SIN with or without the specific caspase-3 inhibitor Ac-DEVD-CHO (10 μM) for 24 h. At the end of the treatment, the cells are washed with PBS and are stained for 15 min with 10 μM Hoechst 33258 dye. Images of the staineing cells are captured with a fluorescent microscope. The differences are evaluated by counting the number of cells with apoptotic nuclear condensation in each well[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [5]

    One hundred and two male mice are subjected to cecal ligation and puncture or sham operation. The animals are assigned into three equal groups (n=34) according to random number table: sham group, model group, and caspase-3 inhibitor (CI) group. Thirty minutes before CLP, Ac-DEVD-CHO (4 μg/g) is injected subcutaneously in CI group. The levels of blood urea nitrogen (BUN) and creatinine (Cr) are determined, and the concentrations of tumor necrosis factor-α (TNF-α), interleukins (IL-6 and IL-10) are measured by enzyme linked immunosorbent assay (ELISA), the renal cell apoptosis rate is determined by flow cytometry. The 4-day and 7-day survival rates of three groups of mice are observed[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.9902 mL 9.9508 mL 19.9017 mL 49.7542 mL
    5 mM 0.3980 mL 1.9902 mL 3.9803 mL 9.9508 mL
    10 mM 0.1990 mL 0.9951 mL 1.9902 mL 4.9754 mL
    15 mM 0.1327 mL 0.6634 mL 1.3268 mL 3.3169 mL
    20 mM 0.0995 mL 0.4975 mL 0.9951 mL 2.4877 mL
    25 mM 0.0796 mL 0.3980 mL 0.7961 mL 1.9902 mL
    30 mM 0.0663 mL 0.3317 mL 0.6634 mL 1.6585 mL
    40 mM 0.0498 mL 0.2488 mL 0.4975 mL 1.2439 mL
    50 mM 0.0398 mL 0.1990 mL 0.3980 mL 0.9951 mL
    60 mM 0.0332 mL 0.1658 mL 0.3317 mL 0.8292 mL
    80 mM 0.0249 mL 0.1244 mL 0.2488 mL 0.6219 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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