Ac-DEVD-CHO
Based on 47 publication(s) in Google Scholar
Ac-DEVD-CHO is a Caspase-3 inhibitor with a Ki value of 230 pM.
For research use only. We do not sell to patients.
- Purity: 99.76%
- CAS No.: 169332-60-9
- Formula: C20H30N4O11
- Molecular Weight:502.47
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications Citing Use of MedChemExpress (MCE) Ac-DEVD-CHO
More- Cell. 2025 May 29;188(11):3013-3029.e19. [Abstract]
- Adv Mater. 2023 Jun;35(23):e2300548. [Abstract]
- Biomaterials. 2022 Sep 29;290:121832. [Abstract]
- Sci Adv. 2025 May 16;11(20):eadq3870. [Abstract]
- Redox Biol. 2024 Jun 8:74:103225. [Abstract]
- J Control Release. 2021 Jun 10:334:21-33. [Abstract]
- J Immunother Cancer. 2024 Mar 1;12(3):e008054. [Abstract]
- Cancer Lett. 2021 Nov 1:520:143-159. [Abstract]
- J Colloid Interface Sci. 2022 Jan 15;606(Pt 2):1950-1965. [Abstract]
- Cell Death Dis. 2025 Jan 26;16(1):44. [Abstract]
- Cell Death Dis. 2022 Aug 18;13(8):721. [Abstract]
- Int J Biol Macromol. 2025 Sep 4;327(Pt 2):147256. [Abstract]
- Int J Biol Macromol. 2025 May;306(Pt 3):141587. [Abstract]
- Apoptosis. 2019 Oct;24(9-10):703-717. [Abstract]
- Apoptosis. 2019 Apr;24(3-4):312-325. [Abstract]
- Basic Res Cardiol. 2021 Jan 23;116(1):3. [Abstract]
- Diabetes. 2022 Apr 1;71(4):637-652. [Abstract]
- Cell Death Discov. 2024 Mar 8;10(1):122. [Abstract]
- Cell Rep. 2025 Sep 12;44(9):116286. [Abstract]
- Appl Mater Today. 2021, 101066.
- J Agric Food Chem. 2023 Oct 18;71(41):15287-15295. [Abstract]
- J Agric Food Chem. 2019 Oct 23;67(42):11718-11727. [Abstract]
- Eur J Med Chem. 2025 May 5:289:117485. [Abstract]
- Int J Mol Med. 2024 Nov;54(5):96. [Abstract]
- Biochem Pharmacol. 2021 Jun:188:114538. [Abstract]
- Cell Prolif. 2019 Sep;52(5):e12663. [Abstract]
- Chem Biol Interact. 2021 Dec 1:350:109704. [Abstract]
- Toxicology. 2024 Aug 6:153906. [Abstract]
- Exp Neurol. 2025 Nov:393:115371. [Abstract]
- Sci Rep. 2025 Apr 18;15(1):13377. [Abstract]
- Sci Rep. 2025 Jan 11;15(1):1654. [Abstract]
- J Virol. 2026 May 19;100(5):e0011726. [Abstract]
- Arch Pharm (Weinheim). 2025 Jul;358(7):e70031. [Abstract]
- Toxicol Appl Pharmacol. 2025 Dec 8:507:117682. [Abstract]
- Acta Biochim Biophys Sin (Shanghai). 2022 Jan 25;54(1):64-76. [Abstract]
- Cell Biol Int. 2020 Apr;44(4):937-946. [Abstract]
- Biochimie. 2019 Dec;167:217-227. [Abstract]
- Vet Microbiol. 2021 Oct:261:109177. [Abstract]
- PLoS One. 2019 Dec 30;14(12):e0227278. [Abstract]
- Curr Protoc. 2026 Jan;6(1):e70288. [Abstract]
- Genet Mol Biol. 2024 Mar 4;47(1):e20220170. [Abstract]
- Biosci Biotechnol Biochem. 2020 May;84(5):989-996. [Abstract]
- J Tradit Chinese Medical Sci. 2024 Jun 5.
- Research Square Preprint. 2024 Apr 12.
- SSRN. 2023 Nov 8.
- Research Square Preprint. 2023 Oct 23.
- Universitat Politècnica de València. 2020 Sep 7.
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Cell Proliferation/Viability Assay
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WB
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
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Cell Imaging/Staining
All Caspase Isoforms
More
Biological Activity
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Caspase 3 0.23 nM (Ki) |
Caspase-8 0.92 nM (Ki) |
Caspase-7 1.6 nM (Ki) |
Caspase-10 12 nM (Ki) |
Caspase-1 18 nM (Ki) |
Caspase-6 31 nM (Ki) |
Caspase-9 60 nM (Ki) |
Caspase-4 132 nM (Ki) |
Caspase-5 205 nM (Ki) |
Caspase-2 1710 nM (Ki) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| NT2 | EC50 |
>100 μM
Compound: Ac-DEVD-CHO
|
Inhibition of camptothecin induced apoptosis in human NT2 cells incubated for 5 hrs in presence of camptothecin by ELISA method
Inhibition of camptothecin induced apoptosis in human NT2 cells incubated for 5 hrs in presence of camptothecin by ELISA method
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[PMID: 33569941] |
| NT2 | IC50 |
>100 μM
Compound: 3
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Inhibitory concentration required against NT2 cells
Inhibitory concentration required against NT2 cells
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[PMID: 12798321] |
| NT2 | IC50 |
>100 μM
Compound: Ac-DEVD-CHO
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Inhibitory activity against NT2 cells
Inhibitory activity against NT2 cells
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[PMID: 14741294] |
| THP-1 | IC50 |
7.0 x 10-5M
Compound: 2a
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Amidolytic activity of THP.1 cell lysate against IL-1 beta converting enzyme using C1-Asp-AMC as a substrate
Amidolytic activity of THP.1 cell lysate against IL-1 beta converting enzyme using C1-Asp-AMC as a substrate
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[PMID: 9873373] |
To ascertain the role of caspase-3 in SLNT-induced apoptosis, a caspase-3 inhibitor (Ac-DEVD-CHO) is used. The addition of Ac-DEVD-CHO significantly prevents SLNT-induced apoptosis (from 32.91±1.21% decreases to 15.88±1.58% while NC and Ac-DEVD-CHO groups are 6.45±0.96%, 7.77±0.79%, respectively)[2].
The apoptosis rates of cells pretreated with zVAD-fmk (5.32%) or Ac-DEVD-CHO (7.43%) decrease obviously after hypericin-mediated PDT treatment[3].
Remarkably, 10 μmol/L Ac-DEVD-CHO partially blocks the effect of SIN-induced apoptosis and reduces the number of apoptotic nuclei. These effects of SIN are blocked by the caspase-3 inhibitor Ac-DEVD-CHO. Camptothecin (4 μM), a positive control, increases caspase-3 activity, which is also blocked by Ac-DEVD-CHO[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 169332-60-9
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Appearance Solid
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Molecular Weight 502.47
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Formula C20H30N4O11
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Color White to off-white
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Sequence
N-Acetyl-Asp-Glu-Val-Asp-al
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Sequence Shortening
Ac-DEVD-al
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (47)
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Journal Impact Factor
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Most Recent
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Cell
2025 May 29;188(11):3013-3029.e19. PMID: 40252640 -
Adv Mater
Impairing Tumor Metabolic Plasticity via a Stable Metal-Phenolic-Based Polymeric Nanomedicine to Suppress Colorectal Cancer. [Abstract]2023 Jun;35(23):e2300548. PMID: 36917817 -
Biomaterials
Oxygen-boosted biomimetic nanoplatform for synergetic phototherapy/ferroptosis activation and reversal of immune-suppressed tumor microenvironment. [Abstract]2022 Sep 29;290:121832. PMID: 36228518 -
Sci Adv
Endogenous/exogenous dual-responsive nanozyme for photothermally enhanced ferroptosis-immune reciprocal synergistic tumor therapy. [Abstract]2025 May 16;11(20):eadq3870. PMID: 40367177
Ac-DEVD-CHO purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 May 16;11(20):eadq3870. [Abstract]
Ac-DEVD-CHO (DEVD) (50 μM) rescued 4T1 cells from MCMSFT-induced cell death.
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Redox Biol
FAM3A plays a key role in protecting against tubular cell pyroptosis and acute kidney injury. [Abstract]2024 Jun 8:74:103225. PMID: 38875957 -
J Control Release
Smart biomimetic metal organic frameworks based on ROS-ferroptosis-glycolysis regulation for enhanced tumor chemo-immunotherapy. [Abstract]2021 Jun 10:334:21-33. PMID: 33872626 -
J Immunother Cancer
Mitochondria-targeted photodynamic therapy triggers GSDME-mediated pyroptosis and sensitizes anti-PD-1 therapy in colorectal cancer. [Abstract]2024 Mar 1;12(3):e008054. PMID: 38429070
Ac-DEVD-CHO purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2024 Mar 1;12(3):e008054. [Abstract]
Ac-DEVD-CHO (100 µM; 4 h), a specific inhibitor of CASP3, prevented the activation of CASP3 and inhibited the cleavage of GSDME in CT26 and HCT116 cells on IR700DX-6T-PDT.
Ac-DEVD-CHO purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2024 Mar 1;12(3):e008054. [Abstract]
Ac-DEVD-CHO (100 µM; 4 h), a specific inhibitor of CASP3, attenuated the release of LDH in CT26 and HCT116 cells on IR700DX-6T-PDT.
Ac-DEVD-CHO purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2024 Mar 1;12(3):e008054. [Abstract]
Ac-DEVD-CHO (100 µM; 4 h) markedly reduced the number of swollen pyroptotic cells in CT26 and HCT116 cells on IR700DX-6T-PDT. Red arrows represent pyroptotic cells.
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Cancer Lett
Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis. [Abstract]2021 Nov 1:520:143-159. PMID: 34256094
Ac-DEVD-CHO purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2021 Nov 1:520:143-159. [Abstract]
Ac-DEVD-CHO (DEVD) (10 mM) decreased the pyroptosis ratio and the formation of pyroptotic-like cells in Eca109 and Ec9706 cells.
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J Colloid Interface Sci
Reactive oxygen species / photothermal therapy dual-triggered biomimetic gold nanocages nanoplatform for combination cancer therapy via ferroptosis and tumor-associated macrophage repolarization mechanism. [Abstract]2022 Jan 15;606(Pt 2):1950-1965. PMID: 34695762 -
Cell Death Dis
ZBP1-mediated PANoptosis is a crucial lethal form in diverse keratinocyte death modalities in UVB-induced skin injury. [Abstract]2025 Jan 26;16(1):44. PMID: 39863598 -
Cell Death Dis
Whole transcriptome and proteome analyses identify potential targets and mechanisms underlying tumor treating fields against glioblastoma. [Abstract]2022 Aug 18;13(8):721. PMID: 35982032 -
Int J Biol Macromol
Pleurotus citrinopileatus polysaccharides ameliorate DSS-induced ulcerative colitis by improving intestinal barrier and inflammation modulation via NF-κB pathway. [Abstract]2025 Sep 4;327(Pt 2):147256. PMID: 40914366 -
Int J Biol Macromol
Z-VAD(OMe)-FMK suppresses Seneca Valley Virus replication by targeting the active sites of the 3C protease. [Abstract]2025 May;306(Pt 3):141587. PMID: 40032110 -
Apoptosis
ATP induces caspase-3/gasdermin E-mediated pyroptosis in NLRP3 pathway-blocked murine macrophages. [Abstract]2019 Oct;24(9-10):703-717. PMID: 31175486
Ac-DEVD-CHO purchased from MedChemExpress. Usage Cited in: Apoptosis. 2019 Oct;24(9-10):703-717. [Abstract]
BMDMs were first primed with LPS (500 ng/ml) for 4 h, pre-treated with or without MCC950 (1 μM) and caspase-3 inhibitor Ac-DEVD-CHO (DEVD) for 1 h and followed by stimulation with ATP (5 mM) in the presence or absence of MCC950. The cell lysates are collected and western blotting is performed to assess the expression levels of indicated proteins.
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Apoptosis
Chemotherapeutic paclitaxel and cisplatin differentially induce pyroptosis in A549 lung cancer cells via caspase-3/GSDME activation. [Abstract]2019 Apr;24(3-4):312-325. PMID: 30710195 -
Basic Res Cardiol
Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1. [Abstract]2021 Jan 23;116(1):3. PMID: 33484341 -
Diabetes
Lactate Is a Key Mediator That Links Obesity to Insulin Resistance via Modulating Cytokine Production From Adipose Tissue. [Abstract]2022 Apr 1;71(4):637-652. PMID: 35044451 -
Cell Death Discov
2024 Mar 8;10(1):122. PMID: 38458999 -
Cell Rep
Pathogenic phosphorylation of linear ubiquitin machinery causes inflammasome sensor degradation. [Abstract]2025 Sep 12;44(9):116286. PMID: 40944911 -
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J Agric Food Chem
2023 Oct 18;71(41):15287-15295. PMID: 37788342 -
J Agric Food Chem
2019 Oct 23;67(42):11718-11727. PMID: 31518118 -
Eur J Med Chem
Exosome/liposome hybrid nanovesicles for enhanced phototherapy and boosted anti-tumor immunity against melanoma. [Abstract]2025 May 5:289:117485. PMID: 40081104 -
Int J Mol Med
TREM2, a critical activator of pyroptosis, mediates the anti‑tumor effects of piceatannol in uveal melanoma cells via caspase 3/GSDME pathway. [Abstract]2024 Nov;54(5):96. PMID: 39219277 -
Biochem Pharmacol
Homoharringtonine synergizes with quizartinib in FLT3-ITD acute myeloid leukemia by targeting FLT3-AKT-c-Myc pathway. [Abstract]2021 Jun:188:114538. PMID: 33831397 -
Cell Prolif
Caspase-3-mediated GSDME activation contributes to cisplatin- and doxorubicin-induced secondary necrosis in mouse macrophages. [Abstract]2019 Sep;52(5):e12663. PMID: 31347748 -
Chem Biol Interact
Dihydroartemisinin mediating PKM2-caspase-8/3-GSDME axis for pyroptosis in esophageal squamous cell carcinoma. [Abstract]2021 Dec 1:350:109704. PMID: 34655567 -
Toxicology
Proteasome inhibition induces apoptosis through simultaneous inactivation of MCL-1/BCL-XL by NOXA independent of CHOP and JNK pathways. [Abstract]2024 Aug 6:153906. PMID: 39117261 -
Exp Neurol
KIF23 inhibition protects against perioperative neurocognitive disorders by hindering ROS/caspase-3/GSDME-mediated pyroptosis. [Abstract]2025 Nov:393:115371. PMID: 40639436 -
Sci Rep
Caerin 1.1 and 1.9 peptides induce acute caspase 3/GSDME-mediated pyroptosis in epithelial cancer cells. [Abstract]2025 Apr 18;15(1):13377. PMID: 40251208 -
Sci Rep
RSL3 induces ferroptosis by activating the NF-κB signalling pathway to enhance the chemosensitivity of triple-negative breast cancer cells to paclitaxel. [Abstract]2025 Jan 11;15(1):1654. PMID: 39794456 -
J Virol
Febrifugine dihydrochloride restricts porcine epidemic diarrhea virus replication by modulating the IGF1R-driven PI3K/AKT-apoptosis axis. [Abstract]2026 May 19;100(5):e0011726. PMID: 41983768 -
Arch Pharm (Weinheim)
2025 Jul;358(7):e70031. PMID: 40702793 -
Toxicol Appl Pharmacol
2025 Dec 8:507:117682. PMID: 41371369 -
Acta Biochim Biophys Sin (Shanghai)
Induction of multiple subroutines of regulated necrosis in murine macrophages by natural BH3-mimetic gossypol. [Abstract]2022 Jan 25;54(1):64-76. PMID: 35130622 -
Cell Biol Int
DJ-1 inhibits autophagy activity of prostate cancer cells by repressing JNK-Bcl2-Beclin1 signaling. [Abstract]2020 Apr;44(4):937-946. PMID: 31868268 -
Biochimie
Autophagy mediated by JNK1 resists apoptosis through TRAF3 degradation in osteoclastogenesis. [Abstract]2019 Dec;167:217-227. PMID: 31654668 -
Vet Microbiol
Lipid metabolism is a novel and practical source of potential targets for antiviral discovery against porcine parvovirus. [Abstract]2021 Oct:261:109177. PMID: 34391196 -
PLoS One
2019 Dec 30;14(12):e0227278. PMID: 31887216 -
Curr Protoc
Overcoming Low-Field Limitations: High-Quality Ex Vivo Soft Tissue Imaging with Compact MRI Systems. [Abstract]2026 Jan;6(1):e70288. PMID: 41481274 -
Genet Mol Biol
The inhibition of Beclin1-dependent autophagy sensitizes PTC cells to ABT737-induced death. [Abstract]2024 Mar 4;47(1):e20220170. PMID: 38488525 -
Biosci Biotechnol Biochem
Autophagy promotion enhances the protective effect of Morroniside on human OA chondrocyte. [Abstract]2020 May;84(5):989-996. PMID: 31983285 -
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Solvent & Solubility
H2O : ≥ 50 mg/mL (99.51 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (199.02 mM); Clear solution; Need ultrasonic
Protocol
OCLs are incubated with RANKL and treated with 0.5 mM SIN with or without the specific caspase-3 inhibitor Ac-DEVD-CHO (10 μM) for 24 h. At the end of the treatment, the cells are washed with PBS and are stained for 15 min with 10 μM Hoechst 33258 dye. Images of the staineing cells are captured with a fluorescent microscope. The differences are evaluated by counting the number of cells with apoptotic nuclear condensation in each well[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
One hundred and two male mice are subjected to cecal ligation and puncture or sham operation. The animals are assigned into three equal groups (n=34) according to random number table: sham group, model group, and caspase-3 inhibitor (CI) group. Thirty minutes before CLP, Ac-DEVD-CHO (4 μg/g) is injected subcutaneously in CI group. The levels of blood urea nitrogen (BUN) and creatinine (Cr) are determined, and the concentrations of tumor necrosis factor-α (TNF-α), interleukins (IL-6 and IL-10) are measured by enzyme linked immunosorbent assay (ELISA), the renal cell apoptosis rate is determined by flow cytometry. The 4-day and 7-day survival rates of three groups of mice are observed[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (285 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Garcia-Calvo M, et al.nhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem. 1998 Dec 4;273(49):32608-13. [Content Brief]
[2]. Jinglin Wang, et al. A polysaccharide from Lentinus edodes inhibits human colon cancer cell proliferation and suppresses tumor growth in athymic nude mice. Oncotarget. 2017 Jan 3; 8(1): 610-623. [Content Brief]
[3]. Junping Zhang, et al. Hypericin-mediated photodynamic therapy induces apoptosis of myoloma SP2/0 cells depended on caspase activity in vitro. Cancer Cell Int. 2015; 15: 58 [Content Brief]
[4]. Long-gang He, et al. Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation. Acta Pharmacol Sin. 2014 Feb; 35(2): 203-210. [Content Brief]
[5]. Liu LX, et al. The effect of caspase-3 inhibitor on the concentrations of serum inflammatory cytokines in sepsis related acute kidney injury induced by peritoneal cavity infection in mice. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Dec;22(12):736-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.9902 mL | 9.9508 mL | 19.9017 mL | 49.7542 mL |
| 5 mM | 0.3980 mL | 1.9902 mL | 3.9803 mL | 9.9508 mL | |
| 10 mM | 0.1990 mL | 0.9951 mL | 1.9902 mL | 4.9754 mL | |
| 15 mM | 0.1327 mL | 0.6634 mL | 1.3268 mL | 3.3169 mL | |
| 20 mM | 0.0995 mL | 0.4975 mL | 0.9951 mL | 2.4877 mL | |
| 25 mM | 0.0796 mL | 0.3980 mL | 0.7961 mL | 1.9902 mL | |
| 30 mM | 0.0663 mL | 0.3317 mL | 0.6634 mL | 1.6585 mL | |
| 40 mM | 0.0498 mL | 0.2488 mL | 0.4975 mL | 1.2439 mL | |
| 50 mM | 0.0398 mL | 0.1990 mL | 0.3980 mL | 0.9951 mL | |
| 60 mM | 0.0332 mL | 0.1658 mL | 0.3317 mL | 0.8292 mL | |
| 80 mM | 0.0249 mL | 0.1244 mL | 0.2488 mL | 0.6219 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.