Nile Red (solution)

Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
1. Preparation of working solution
Dilute the stored solution with preheated serum-free cell culture medium or PBS to a final concentration of 200-1000 nM.
Note: Please adjust the concentration of Nile Red working solution according to the actual situation, and prepare it as needed.
2. Cell staining
2.1 Suspension cells: Centrifuge to collect cells, add PBS for two washes, each for 5 minutes.
Adherent cells: Discard the culture medium, add trypsin to digest the cells. Centrifuge and discard the supernatant, then add PBS for two washes, each for 5 minutes.
2.2 Add 1 mL of Nile Red working solution, incubate at room temperature for 5-10 minutes.
2.3 Centrifuge at 400 g for 3-4 minutes at 4℃, discard the supernatant.
2.4 Add PBS for two washes of the cells, each for 5 minutes.
2.5 Resuspend the cells in 1 mL of serum-free medium or PBS, and observe using a fluorescence microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.