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  4. Nile Red

Nile Red  (Synonyms: Nile Blue A oxazone; Phenoxazone 9)

Cat. No.: HY-D0718 Purity: 99.74%
COA
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Handling Instructions Technical Support

Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm.

For research use only. We do not sell to patients.

CAS No. : 7385-67-3

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Customer Review

Based on 116 publication(s) in Google Scholar

Other Forms of Nile Red:

Nile Red Related Antibodies

Top Publications Citing Use of Products

116 Publications Citing Use of MCE Nile Red

IF

    Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Oct 5;13(1):5871.  [Abstract]

    Isolated oocytes are fixed with 4% paraformaldehyde for 1 hour at room temperature and washed 3 times with 1% BSA in PBS. Then, they are stained with 10 µg/ml Nile red at 4 °C overnight.

    Nile Red purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 Jun 24;50(12):6953-6967.  [Abstract]

    The wild-type or G4-mutant human hepatic adenocarcinoma HepG2 cells are stained with Nile Red reagent to visualize the lipids with an Olympus Fluoview FV1000 confocal microscope.

    Nile Red purchased from MedChemExpress. Usage Cited in: Nano Today. 47 (2022) 101675

    Subcellular biodistribution of NileRed-labeled PFD Liposome nano-particles in 4T1 and BT-549 cells are visualized by confocal laser microscopy. Blue, DAPI; Green, WGA 488; Red, NileRed-labeled PFD Liposome.

    Nile Red purchased from MedChemExpress. Usage Cited in: EMBO J. 2022 Aug 16;41(16):e110439.  [Abstract]

    Representative pictures of Nile red-stained human BAs differentiated from hESC-derived brown progenitors.

    Nile Red purchased from MedChemExpress. Usage Cited in: J Transl Med. 2022 May 14;20(1):222.  [Abstract]

    Living cells are incubated with Nile Red (1 μM) for 15 min at 37 °C in dark. Then, cells are washed with HBSS/Ca/Mg and analyzed by fluorescence microscopy.

    Nile Red purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2023 Jan 5:938:175428.  [Abstract]

    Hepatic lipid and FFA content are determined by Nile red staining of liver frozen sections.

    Nile Red purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Jan 1;11(5):2149-2169.  [Abstract]

    Injected 5 μg of Nile Red lipid dye into the tail vein of mice to label triglycerides in the liver prior to intravital imaging.

    Nile Red purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2021 Nov;54(11):e13134.  [Abstract]

    For lipid droplet staining, fixed cells are stained for 30 minutes with 0.1 µg/ml Nile Red (MCE), and the nucleus is counterstained using DAPI.

    Nile Red purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2021 May 31;22(11):5951.  [Abstract]

    For lipid staining, fat bodies are submerged in Nile red solution at a final working concentration of 10 μg/mL in an acetone/water (1:9) mixture and visualized using a fluorescent microscope at Ex543/Em626 nm.

    Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Jan 13;11(1):240.   [Abstract]

    HSCs are stained with Nile Red reagents and Bodipy493/503 to visualize the lipids with a light microscope.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm[1].

    Cellular Effect
    Cell Line Type Value Description References
    L6 IC50
    65.9 μM
    Compound: 7
    Cytotoxicity against rat L6 cells
    Cytotoxicity against rat L6 cells
    		[PMID: 24900219]
    In Vitro

    Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
    1. Preparation of storage solution and working solution
    1.1 Preparation of storage solution
    Prepare 1 mM storage solution using DMSO.
    1.2 Preparation of working solution
    Dilute the storage solution with pre-heated serum-free cell culture medium or PBS to a final concentration of 200-1000 nM.
    Note: Please adjust the concentration of Nile Red working solution according to the actual situation, and prepare it as needed.
    2. Cell staining
    2.1 Suspension cells: Centrifuge to collect cells, add PBS for two washes, each for 5 minutes.
    Adherent cells: Discard the culture medium, add trypsin to digest the cells. Centrifuge and discard the supernatant, then add PBS for two washes, each for 5 minutes.
    2.2 Add 1 mL of Nile Red working solution, incubate at room temperature for 5-10 minutes.
    2.3 400 g, 4℃ centrifuge for 3-4 minutes, discard the supernatant.
    2.4 Add PBS for two washes of the cells, each for 5 minutes.
    2.5 Resuspend the cells in 1 mL of serum-free medium or PBS, and observe using a fluorescence microscope.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nile Red Related Antibodies
    In Vivo

    When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    318.37

    Formula

    C20H18N2O2
    CAS No.
    Appearance

    Solid

    Color

    Green to dark green

    Emission (Em)

    598
    Excitation (Ex)

    543
    SMILES

    O=C1C2=CC=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 2 mg/mL (6.28 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 1 mg/mL (3.14 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1410 mL 15.7050 mL 31.4100 mL
    5 mM 0.6282 mL 3.1410 mL 6.2820 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.74%

    SDS (393 KB)

    COA (250 KB) HNMR (127 KB) RP-HPLC (262 KB) LCMS (235 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 3.1410 mL 15.7050 mL 31.4100 mL 78.5250 mL
    DMSO 5 mM 0.6282 mL 3.1410 mL 6.2820 mL 15.7050 mL
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    Nile Red Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nile Red7385-67-3Nile Blue A oxazone Phenoxazone 9Phenoxazone9Phenoxazone 9Phenoxazone-9Fluorescent DyeBenzophenoxazonehydrophobiclipiddropletsfluorescentneutrallysochromeInhibitorinhibitorinhibit

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