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Nile Red  (Synonyms: Nile Blue A oxazone; Phenoxazone 9)

Cat. No.: HY-D0718 Purity: 98.15%
COA Handling Instructions

Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm.

For research use only. We do not sell to patients.

Nile Red Chemical Structure

Nile Red Chemical Structure

CAS No. : 7385-67-3

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Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Solid
5 mg USD 35 In-stock
10 mg USD 55 In-stock
50 mg USD 72 In-stock
100 mg USD 83 In-stock
500 mg USD 132 In-stock
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Customer Review

Based on 46 publication(s) in Google Scholar

Top Publications Citing Use of Products

36 Publications Citing Use of MCE Nile Red

IF

    Nile Red purchased from MedChemExpress. Usage Cited in: Nano Today. 47 (2022) 101675

    Subcellular biodistribution of NileRed-labeled PFD Liposome nano-particles in 4T1 and BT-549 cells are visualized by confocal laser microscopy. Blue, DAPI; Green, WGA 488; Red, NileRed-labeled PFD Liposome.

    Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 Oct 5;13(1):5871.  [Abstract]

    Isolated oocytes are fixed with 4% paraformaldehyde for 1 hour at room temperature and washed 3 times with 1% BSA in PBS. Then, they are stained with 10 µg/ml Nile red at 4 °C overnight.

    Nile Red purchased from MedChemExpress. Usage Cited in: Nucleic Acids Res. 2022 Jun 24;50(12):6953-6967.  [Abstract]

    The wild-type or G4-mutant human hepatic adenocarcinoma HepG2 cells are stained with Nile Red reagent to visualize the lipids with an Olympus Fluoview FV1000 confocal microscope.

    Nile Red purchased from MedChemExpress. Usage Cited in: EMBO J. 2022 Jul 4;e110439.  [Abstract]

    Representative pictures of Nile red-stained human BAs differentiated from hESC-derived brown progenitors.

    Nile Red purchased from MedChemExpress. Usage Cited in: J Transl Med. 2022 May 14;20(1):222.  [Abstract]

    Living cells are incubated with Nile Red (1 μM) for 15 min at 37 °C in dark. Then, cells are washed with HBSS/Ca/Mg and analyzed by fluorescence microscopy.

    Nile Red purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Nov 24;175428.  [Abstract]

    Hepatic lipid and FFA content are determined by Nile red staining of liver frozen sections.

    Nile Red purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Jan 1;11(5):2149-2169.  [Abstract]

    Injected 5 μg of Nile Red lipid dye into the tail vein of mice to label triglycerides in the liver prior to intravital imaging.

    Nile Red purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2021 Sep 25;e13134.  [Abstract]

    For lipid droplet staining, fixed cells are stained for 30 minutes with 0.1 µg/ml Nile Red (MCE), and the nucleus is counterstained using DAPI.

    Nile Red purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2021 May 31;22(11):5951.  [Abstract]

    For lipid staining, fat bodies are submerged in Nile red solution at a final working concentration of 10 μg/mL in an acetone/water (1:9) mixture and visualized using a fluorescent microscope at Ex543/Em626 nm.

    Nile Red purchased from MedChemExpress. Usage Cited in: Nat Commun. 2020 Jan 13;11(1):240.   [Abstract]

    HSCs are stained with Nile Red reagents and Bodipy493/503 to visualize the lipids with a light microscope.
    • Biological Activity

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    Description

    Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm[1].

    In Vitro

    1. Preparation of Phalloidin-TRITC working solution
    1.1Preparation of the stock solution
    Dissolve Phalloidin-TRITC in Methanol to obtain 10 mM of stock solution.
    Note: It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
    1.2 Preparation of Phalloidin-TRITC working solution
    Dilute the stock solution in serum-free cell culture medium to obtain 1-10 μM of working solution.
    Note: Please adjust the concentration of Phalloidin-TRITC working solution according to the actual situation.
    2. Cell staining
    2.1 Suspension cells (6-well plate)
    a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    b.Add 1 mL of working solution, and then incubate at room temperature for 30-60 minutes.
    c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
    d.Wash twice with PBS, 5 minutes each time.
    e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    2.2 Adherent cells
    a.Culture adherent cells on sterile coverslips.
    b.Remove the coverslip from the medium and aspirate excess medium.
    c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
    d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    318.37

    Formula

    C20H18N2O2

    CAS No.
    Appearance

    Solid

    Emission (Em)

    598

    Excitation (Ex)

    543

    SMILES

    O=C1C2=CC=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, protect from light

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 2 mg/mL (6.28 mM; ultrasonic and warming and heat to 60°C)

    Ethanol : 1 mg/mL (3.14 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1410 mL 15.7050 mL 31.4100 mL
    5 mM 0.6282 mL 3.1410 mL 6.2820 mL
    10 mM --- --- ---
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 0.2 mg/mL (0.63 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.2 mg/mL (0.63 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.15%

    Dyeing Example
    References
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    Nile Red Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    Product Name:
    Nile Red
    Cat. No.:
    HY-D0718
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