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  2. Nile Red

Nile Red (Synonyms: Nile Blue A oxazone; Phenoxazone 9)

Cat. No.: HY-D0718 Purity: 98.02%
Handling Instructions

Nile Red (Nile Blue A oxazone) is a selective and hydrophobic fluorescent stain for intracellular lipid droplets and neutral lipids. Nile Red is intensely fluorescent in all organic solvents and the fluorescence colors range from golden yellow to deep red.

For research use only. We do not sell to patients.

Nile Red Chemical Structure

Nile Red Chemical Structure

CAS No. : 7385-67-3

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10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
10 mg USD 50 In-stock
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50 mg USD 65 In-stock
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100 mg USD 75 In-stock
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500 mg USD 120 In-stock
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Based on 5 publication(s) in Google Scholar

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Description

Nile Red (Nile Blue A oxazone) is a selective and hydrophobic fluorescent stain for intracellular lipid droplets and neutral lipids. Nile Red is intensely fluorescent in all organic solvents and the fluorescence colors range from golden yellow to deep red[1][2].

In Vitro

Nile red-stained, lipid droplet-filled macrophages exhibit greater fluorescence intensity than does Nile red-stained control macrophages, and the two cell populations could be differentiated and analyzed by flow cytofluorometry. Better selectivity for cytoplasmic lipid droplets is obtained when the cells are viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm)[1].
Nile red is strongly fluorescent, but only in the presence of a hydrophobic environment. Nile red is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution[1].
Spectral and physicochemical properties of the lipophilic dye Nile red induce a yellow-gold-spectral shift in its excitation-emission peak, allowing it to fluoresce in the green emission spectrum only when in a lipid-rich environment, but not in more polar environments[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

318.37

Formula

C₂₀H₁₈N₂O₂

CAS No.

7385-67-3

SMILES

O=C1C2=CC=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (157.05 mM)

H2O : 0.1 mg/mL (0.31 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1410 mL 15.7050 mL 31.4100 mL
5 mM 0.6282 mL 3.1410 mL 6.2820 mL
10 mM 0.3141 mL 1.5705 mL 3.1410 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Keywords:

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Nile Red
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HY-D0718
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