FITC-Dextran (MW 70000)
Based on 12 publication(s) in Google Scholar
FITC-Dextran (MW 70000) is a compound belonging to the class of fluorescent dyes. It is commonly used in biomedical research as a tracer molecule to label and track cells or other biological matter. FITC-Dextran consists of fluorescein isothiocyanate (FITC) and dextran, a complex carbohydrate derived from starch. The combination of the two creates a stable fluorescent tracer that can be viewed under a microscope or quantified using specialized detection instruments.
For research use only. We do not sell to patients.
- CAS No.: 60842-46-8
- Molecular Weight:70000(Average)
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications Citing Use of MedChemExpress (MCE) FITC-Dextran (MW 70000)
More- Cancer Cell. 2024 Apr 8;42(4):682-700.e12. [Abstract]
- ACS Nano. 2025 Dec 19. [Abstract]
- J Nanobiotechnology. 2024 Aug 12;22(1):479. [Abstract]
- MedComm (2020). 2026 Apr 9;7(4):e70722. [Abstract]
- Biomaterials. 2025 Sep 17:326:123723. [Abstract]
- Cancer Lett. 2024 Nov 28:605:217288. [Abstract]
- Allergy. 2025 Nov 14. [Abstract]
- Toxicology. 2025 Oct 13:519:154303. [Abstract]
- ACS Omega. 2025 Jun 2;10(23):24284-24295. [Abstract]
- Cell Signal. 2025 Oct:134:111963. [Abstract]
- AMB Express. 2025 Oct 2;15(1):142. [Abstract]
- Exp Eye Res. 2024 Oct:247:110025. [Abstract]
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In Vivo Efficacy Study
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In Vivo Imaging
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In Vivo Imaging
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In Vivo Efficacy Study
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Bio/Physico-chemical Assay
Biological Activity
FITC-Dextran (MW 70000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 70000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 70000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption[1][2][3]. Storage: protect from light.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
For intestinal barrier function assay[5]
1. Fast mice for 4 h.
2. Orally gavage mice with FITC-Dextran MW 70000 (0.6 mg/g).
3. Measure fluorescence intensity of plasma in 4 h (excitation nm/emission 520 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 60842-46-8
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Appearance Solid
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Molecular Weight 70000(Average)
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Color Yellow to orange
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SMILES
[FITC-Dextran (MW 70000)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (12)
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Journal Impact Factor
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Most Recent
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Cancer Cell
PDGFRα+ITGA11+ fibroblasts foster early-stage cancer lymphovascular invasion and lymphatic metastasis via ITGA11-SELE interplay. [Abstract]2024 Apr 8;42(4):682-700.e12. PMID: 38428409 -
ACS Nano
Central Nervous System Targeting Nanovesicles for Trans-Barrier Delivery and Spinal Cord Injury Treatment. [Abstract]2025 Dec 19. PMID: 41420148 -
J Nanobiotechnology
Oral administration of Robinia pseudoacacia L. flower exosome-like nanoparticles attenuates gastric and small intestinal mucosal ferroptosis caused by hypoxia through inhibiting HIF-1α- and HIF-2α-mediated lipid peroxidation. [Abstract]2024 Aug 12;22(1):479. PMID: 39134988
FITC-Dextran (MW 70000) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2024 Aug 12;22(1):479. [Abstract]
Murine intestinal permeability was evaluated by oral administration of FITC-dextran (0.6 mg/g,4 h). RFELNs administration significantly decreased serum FITC levels, suggesting that RFELNs ameliorated hypoxia-induced gastrointestinal mucosal barrier disruption.
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MedComm (2020)
Ipriflavone From Aquilaria malaccensis Lam. Exosome-Like Nanoparticles Targets Prolyl Hydroxylase Domain Protein 2 (PHD2) to Enhance Hypoxia-Inducible Factor-α (HIF-α) Hydroxylation Thereby Alleviating Hypoxia-Induced Gastrointestinal Mucosal Ferroptosis. [Abstract]2026 Apr 9;7(4):e70722. PMID: 41969632 -
Biomaterials
Fully synthetic nanofibrillar hydrogel for the growth and enzyme-free release of patient-derived ovarian tumor organoids. [Abstract]2025 Sep 17:326:123723. PMID: 40976136 -
Cancer Lett
S100A7 orchestrates neutrophil chemotaxis and drives neutrophil extracellular traps (NETs) formation to facilitate lymph node metastasis in cervical cancer patients. [Abstract]2024 Nov 28:605:217288. PMID: 39384116 -
Allergy
Long-Term Attenuation of Vascular Hyperpermeability in a Hereditary Angioedema Mouse Model by Adenine Base Editing. [Abstract]2025 Nov 14. PMID: 41236489 -
Toxicology
The dysbiosis of gut microbiota attributes to the impairment of blood-brain barrier in rats triggered by cadmium. [Abstract]2025 Oct 13:519:154303. PMID: 41075966 -
ACS Omega
Liver-Skin Microphysiological System for Evaluating Topical Drug Delivery-Induced Liver Injury. [Abstract]2025 Jun 2;10(23):24284-24295. PMID: 40547626
FITC-Dextran (MW 70000) purchased from MedChemExpress. Usage Cited in: ACS Omega. 2025 Jun 2;10(23):24284-24295. [Abstract]
The permeability of FITC-dextran (50 μM, 0.5-2 h) across the skin microtissue on the LS-MPS on day 21.
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Cell Signal
Exosomal miR-382-5p prevents pre-metastatic niche formation by inhibiting GPR176/GNAS-CXCR1/CXCR2 axis in colorectal cancer liver metastasis. [Abstract]2025 Oct:134:111963. PMID: 40578589 -
AMB Express
2025 Oct 2;15(1):142. PMID: 41037134
FITC-Dextran (MW 70000) purchased from MedChemExpress. Usage Cited in: AMB Express. 2025 Oct 2;15(1):142. [Abstract]
Gastrointestinal permeability of the indicated group was assessed by serum FITC-dextran (600 mg/kg, 4 h) concentrations.
FITC-Dextran (MW 70000) purchased from MedChemExpress. Usage Cited in: AMB Express. 2025 Oct 2;15(1):142. [Abstract]
The mice in the EO treatment groups were orally administered EOs (1 µg, 5 µg, or 10 µg) on day 1, and euthanized on day 3 (n = 18 per group, with n = 6 in each group specifically for serum FITC-dextran (600 mg/kg, 4 h) detection).
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Exp Eye Res
Pericyte loss via glutaredoxin2 downregulation aggravates diabetes-induced microvascular dysfunction. [Abstract]2024 Oct:247:110025. PMID: 39117135
FITC-Dextran (MW 70000) purchased from MedChemExpress. Usage Cited in: Exp Eye Res. 2024 Oct:247:110025. [Abstract]
Pericyte-endothelial cell cocultures were performed to assess endothelial barrier function, by measuring the passage of 70-kDa FITC–dextran (1 mg/mL, 60 min).
Solvent & Solubility
H2O : 100 mg/mL (Need ultrasonic)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[2]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15. [Content Brief]
[3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189. [Content Brief]
[4]. Okabayashi K, et al. Cdc42 activates paracellular transport in polarised submandibular gland cells. Arch Oral Biol. 2021 Dec;132:105276. [Content Brief]
[5]. Yu W, et al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)