FITC-Dextran (MW 10000)

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FITC-Dextran (MW 10000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 10000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption.

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CAS No. : 60842-46-8

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Based on 90 publication(s) in Google Scholar

Other Forms of FITC-Dextran (MW 10000):

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FITC-Dextran (MW 2000000) In-stock
FITC-Dextran (MW 10000) (solution) In-stock
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FITC-Dextran (MW 150000) In-stock

90 Publications Citing Use of MCE FITC-Dextran (MW 10000)

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In Vivo Efficacy Study

In Vivo Imaging

: FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 18:e00741. [Abstract]; Intestinal permeability was assessed by FITC‐Dextran (0.6 mg/g; Oral gavage) assays on day 10 after DSS initiation (n = 6).

: FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Apr 15;23(1):291. [Abstract]; FITC-dextran (1 mg/mL; 45 min) permeability assay was performed to assess the barrier function of bEnd.3 endothelial cells.

: FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Sci China Life Sci. 2025 Jul;68(7):2010-2027. [Abstract]; Blood FITC-Dextran (MW 4000) (200 mg/kg; Oral gavage; Single dose) levels. The results showed that METTL3-deficient mice presented significantly increased serum FITC levels, indicating increased mucosal permeability.

: FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Jun 4;23(1):263. [Abstract]; Quantitative analysis of FITC in serum collected from mice in the FITC-Dextran (MW 4000) (0.6 mg/g; Oral gavage; Single dose) intestinal permeability assay.

: FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 May 9:144084. [Abstract]; MFI variations with time in Ch1, Ch3 and Ch5 of 10 kDa FITC-dextran (0.1 mg/mL; 0-24 h) diffusion.

: FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Redox Rep. 2024 Dec;29(1):2433393. [Abstract]; Intestinal permeability measured by serum fluorescein isothiocyanate FITC-Dextran (MW 4000) (0.44 mg/g; Oral gavage; Single dose) levels.

: FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2024 Jun 1:591:216897. [Abstract]; Fluorescence intensity of FITC-dextran (500 μg/mL; 36 h) in lower chamber of Transwell after incubation with TM-sEVs from patients with low relapse risk and high relapse risk in the upper chamber.

: FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 20;9(42):eadi0699. [Abstract]; Ex vivo imaging showed biodistribution of FITC-Dextran (MW 4000) (500 μg/mouse; i.v.; Single dose).

: FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2021 Oct;8(20):e2101912. [Abstract]; Large particle transport assays through µE‐BBB were conducted by passing of FITC‐dextran (100 µg/mL; 1 h) solution through the lumen compartment of the system and quantifying the amount of FITC‐dextran that had accumulated in the basolateral compartment.

: FITC-Dextran (MW 10000) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2021 Oct;8(20):e2101912. [Abstract]; To evaluate the effect of erythrocytes on the permeability of the BBB, isolated erythrocytes from young or old mice, supplemented with FITC‐dextran (100 μg/mL; 1 h), were circulated through the µE‐BBB.

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Description	FITC-Dextran (MW 10000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 10000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption^[1]^[2]^[3].
In Vitro	Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs). 1. Cell Marking (for cells undergoing apoptosis)^[1]: 1.1 Resuspend cells in culture medium and add Propidium Iodide (PI) and FITC-Dextran to a final concentration of 7.5 μM and 1.13 μM, respectively. 1.2 Incubate cells at room temperature in the dark for 25 minutes. 1.3 Centrifuge at 500 g for 10 minutes and discard the supernatant. 1.4 Resuspend cells in culture medium and analyze using flow cytometry or fluorescence microscopy (PI: Ex=500 nm, Em=600 nm; FITC-Dextran: Ex=495 nm, Em=525 nm). 2. Side-cell permeability assay^[4] 2.1 Add FITC-Dextran (0.1 mg/mL) to the basal medium in the upper chamber of the transwell. 2.2 Collect the medium from the lower chamber of the transwell after 15 minutes. 2.3 Measure the fluorescence signal (Ex=495 nm, Em=525 nm). 2.4 Calculate the FITC-Dextran concentration based on the fluorescence intensity. 2.5 Calculate the permeability. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. FITC-Dextran (MW 10000) Related Antibodies
In Vivo	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). For intestinal barrier function assay^[5] 1. Fast mice for 4 h. 2. Orally gavage mice with FITC-Dextran MW 10000 (0.6 mg/g). 3. Measure fluorescence intensity of plasma in 4 h (excitation nm/emission 520 nm). MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight	10000.00(Average)
CAS No.	60842-46-8
Appearance	Solid
Color	Yellow to brown
Emission (Em)	525
Excitation (Ex)	488
SMILES	[FITC-Dextran (MW 10000)]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility	In Vitro: H₂O : 50 mg/mL (Need ultrasonic) Molarity Calculator Dilution Calculator Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Mass = Concentration × Volume × Molecular Weight * Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final) This equation is commonly abbreviated as: C₁V₁ = C₂V₂ Concentration _(start) C1 × Volume _(start) V1 = Concentration _(final) C2 × Volume _(final) V2 In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kg Animal weight (per animal) g Dosing volume (per animal) μL Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details. The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). Tel: 609-228-6898 E-mail: [email protected] Technical Support: [email protected]
Purity & Documentation	Select Batch: Data Sheet (280 KB) SDS (393 KB) COA (254 KB) Handling Instructions (2659 KB)
Dyeing Example	Description: FITC-Dextran MW 10000 can be used to explore the cellular uptake with green fluorescence. Method: For cellular uptake monitoring. 1. Incubate cells in glass dishes (37℃; overnight). 2. Incubate cells with FITC-Dextran MW 10000 (5 μg/mL; 4 h). 3. Wash cells for twice with PBS and finally fix cells with 4% PFA (30 min; dark). 4. Use a confocal laser scanning microscopy (Zeiss 880) for image. Description: FITC-Dextran MW 10000 can be used to detect intestinal barrier function in vivo. Method: For assess intestinal barrier function. 1. Fast mice for 4 hours. 2. Orally gavage mice with FITC-Dextran MW 10000 (0.6 mg/g). 3. Measure fluorescence intensity of plasma in 4 hours. Description: FITC-Dextran MW 10000 can be used for labeling microvessels in the lesion area of rat brain to measure microvessel density. Method: For in vivo labeling. 1. Anesthetize rats first. 2. Inject FITC-Dextran MW 10000 (50 mg/kg) in the tail vein of rats. 3. Remove brain tissues 1 min after injection, use 4% paraformaldehyde (4°C; 24 h) to fix tissues and cut tissues into 150 µm-thick sections. 4. Use a laser scanning confocal microscope (Leica, Solms, Germany) for image.
References	[1]. Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1). [2]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15. [Content Brief] [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189. [Content Brief] [4]. Okabayashi K, et al. Cdc42 activates paracellular transport in polarised submandibular gland cells. Arch Oral Biol. 2021 Dec;132:105276. [Content Brief] [5]. Yu W, et al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282. [Content Brief]