FITC-Dextran (MW 10000) (solution) 

FITC-Dextran (MW 10000) (solution) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 10000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption^[1][2][3].
Solvent and Concentration: Sterile water: 2 mM

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).

Labeling of cells^[1]:
Applicable to cells undergoing apoptosis (using HeLa cells and human peripheral blood mononuclear cells (PBMCs) as examples. Note that live HeLa and PBMCs are not stained with FITC-Dextran).
1. Suspend apoptotic cells in 100 μL of culture medium and mix with 10 μL of propidium iodide (PI) and 10 μL of FITC-Dextran (MW 10000) in a Q-prep tube. The final concentrations of PI and FITC-Dextran (MW 10000) are 7.5 μM and 1.13 μM, respectively.
2. Incubate the cells at room temperature in the dark for 25 minutes.
3. Take the labeled cells and centrifuge at 500 g for 10 minutes.
4. Take the centrifuged cells, add 1 mL of culture medium to resuspend them, and analyze them by flow cytometry or fluorescence microscopy (PI: Ex=500 nm, Em=600 nm; FITC-Dextran (MW 10000): Ex=495 nm, Em=525 nm).

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Side-cell permeability detection^[4]
1. Add FITC-Dextran (0.1 mg/mL) to the basal culture medium in the transwell chamber.
2. Collect the culture medium from the transwell inserter after 15 minutes.
3. Measure the fluorescence signal (Ex=495 nm, Em=525 nm).
4. Calculate the FITC-Dextran concentration based on the fluorescence intensity.
5. Calculate the permeability.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
For intestinal barrier function assay^[5]
1. Fast mice for 4 h.
2. Orally gavage mice with FITC-Dextran MW 10000 (0.6 mg/g).
3. Measure fluorescence intensity of plasma in 4 h (excitation nm/emission 520 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

60842-46-8

Liquid

Light yellow to yellow

[FITC-Dextran (MW 10000) (solution)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1).

  [2]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15.  [Content Brief]

  [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189.  [Content Brief]

  [4]. Okabayashi K, et al. Cdc42 activates paracellular transport in polarised submandibular gland cells. Arch Oral Biol. 2021 Dec;132:105276.  [Content Brief]

  [5]. Yu W, et al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282.  [Content Brief]