FITC-Dextran (MW 150000)
Based on 5 publication(s) in Google Scholar
FITC-Dextran (MW 150000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=491 nm; Em=518 nm). FITC-Dextran (MW 150000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 150000) can be used in perfusion studies in animals or in fluorescence microlymphography, to study processes that affect the permeability of the blood brain barrier (BBB). FITC-Dextran (MW 150000) can be used as fluorescent probe to study cell permeability.
For research use only. We do not sell to patients.
- CAS No.: 60842-46-8
- Molecular Weight:150000(Average)
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) FITC-Dextran (MW 150000)
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Customer Validation & Images
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Cell Imaging/Staining
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
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Bio/Physico-chemical Assay
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of cells[1]:
For use with apoptotic HeLa cells and human peripheral blood mononuclear cells (PBMC) (viable HeLa and PBMC can not be stained by FITC-Dextran).
1. Incubate cells at 43.5°C for 1 hour and at 37°C for 8 hours to induce apoptosis.
2. Suspend the cells in 100 μL of medium, and mix in Q-prep tubes with 10 μL of propidium iodide (PI), 10 μL of FITC-Dextran (MW 150000) (the final concentration of PI and FITC-Dextran (MW 150000) is 7.5 μM and 1.13 μM, respectively).
3. Incubate cells for 25 min at room temperature in the dark.
4. Take the labeled cells with 3 mL of medium and centrifuge for 10 min at 500 g.
5. Take centrifuged cells with 1 mL of medium and use flow cytometry or fluorescence microscopy analyze (PI: Ex=500 nm, Em=600 nm; FITC-Dextran (MW 150000): Ex=495 nm, Em=525 nm).
Paracellular permeability measurement[4]
1. Add FITC-dextran (0.1 mg/mL) to the basal media in the transwell chamber.
2. Collect media from the transwell insert after 15 min.
3. Measure the fluorescence signal (Ex=485 nm, Em=538 nm).
4. Calculate FITC-dextran concentration based on fluorescence intensity.
5. Calculate permeability.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
For intestinal barrier function assay[5]
1. Fast mice for 4 h.
2. Orally gavage mice with FITC-Dextran MW 150000 (0.6 mg/g).
3. Measure fluorescence intensity of plasma in 4 h (excitation nm/emission 520 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
525
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Chemical Information
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CAS No. 60842-46-8
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Appearance Solid
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Molecular Weight 150000(Average)
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Color Light yellow to yellow
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SMILES
[FITC-Dextran (MW 150000)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (5)
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Journal Impact Factor
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Most Recent
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Biomaterials
Fully synthetic nanofibrillar hydrogel for the growth and enzyme-free release of patient-derived ovarian tumor organoids. [Abstract]2025 Sep 17:326:123723. PMID: 40976136
FITC-Dextran (MW 150000) purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 Sep 17:326:123723. [Abstract]
The time-dependent variations of normalized fluorescence of FITC-Dextran (40, 70, 150 kDa) (0.5 mg/mL) and FITC-BSA in TNHs with Cp of 70 mg/mL. The inset shows confocal microscopic images of TNHs containing FITC-BSA at different time points after the photo-bleaching in FRAP experiments. The Cp is 70 mg/mL. Scale bar, 50 μm.
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FITC-Dextran (MW 150000) purchased from MedChemExpress. Usage Cited in: Engineering. 2026 Jan 21.
Evaluation of endothelial barrier permeability by monitoring the diffusion of FITC and 40 and 150 kDa FITC-dextran into microspheres in the presence or absence of an HUVEC layer. The results showed that after 2 h of diffusion, the endothelial layer significantly reduced the influx of free FITC compared to the control. Similarly, the penetration of 40 and 150 kDa FITC-dextrans into the hydrogel core was markedly slowed. At the 2-h time point, endothelialized microspheres exhibited significantly lower intramicrosphere fluorescence accumulation than non-endothelialized controls. These findings confirm that the engineered endothelial barrier exerts size-selective regulation over molecular permeation.
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Phytomedicine
Zanthoxylum nitidum-derived active alkaloid nitidine chloride mitigates ulcerative colitis by remodeling gut microbiota and activating SIRT1 to restore intestinal barrier integrity. [Abstract]2026 Jun 15:159:158435. PMID: 42322763 -
Anal Chem
Biomimetic Microparticles with Myocardial and Endocardial Integration for Drug Toxicity Studies. [Abstract]2025 Apr 8. PMID: 40198907
FITC-Dextran (MW 150000) purchased from MedChemExpress. Usage Cited in: Anal Chem. 2025 Apr 8. [Abstract]
Evaluation of endothelial barrier permeability on the microparticles. Time-lapse fluorescence images of the diffusion of FITC, 40 and 150 kDa FITC-Dextran (25 μg/mL; 1 h) into microparticles with or without HUVECs monolayer.
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FASEB J
Zanthoxylum Nitidum Ameliorates Intestinal Barrier Dysfunction and Inflammation in TNBS-Induced Colitis Rats and LPS-Stimulated Caco-2 Cells. [Abstract]2025 Oct 15;39(19):e71099. PMID: 41055302
FITC-Dextran (MW 150000) purchased from MedChemExpress. Usage Cited in: FASEB J. 2025 Oct 15;39(19):e71099. [Abstract]
FITC-Dextran (MW 150000) (1 mg/mL; 60 min) flux method was used to determine the permeability of Caco-2 cells.
Solvent & Solubility
H2O : 66.67 mg/mL (Need ultrasonic)
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[2]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15. [Content Brief]
[3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189. [Content Brief]
[4]. Okabayashi K, et al. Cdc42 activates paracellular transport in polarised submandibular gland cells. Arch Oral Biol. 2021 Dec;132:105276. [Content Brief]
[5]. Yu W, et al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282. [Content Brief]
[6]. Bolliner A,et al.,Fluorescence microlymphography: diagnostic potential in lymphedema and basis for the measurement of lymphatic pressure and flow velocity. Lymphology. 2007 Jun;40(2):52-62. [Content Brief]
[7]. Ishii T, et al., Accumulation of macromolecules in brain parenchyma in acute phase of cerebral infarction/reperfusion. Brain Res. 2010 Mar 19;1321:164-8. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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