FITC-Dextran (MW 40000)

Cat. No.: HY-128868D: Data Sheet
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FITC-Dextran (MW 40000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 40000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 40000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption.

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CAS No. : 60842-46-8

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Based on 12 publication(s) in Google Scholar

12 Publications Citing Use of MCE FITC-Dextran (MW 40000)

Bio/Physico-chemical Assay

Cell Imaging/Staining

In Vivo Imaging

Flow Cytometry

2D/3D Cell Culture and Differentiation

: FITC-Dextran (MW 40000) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Aug;12(30):e00461. [Abstract]; The plasma FD4 level in Acss2^CKO and Acss2^fl/fl mice (n = 7). For intestinal permeability assessment, mice were fasted for 8 h, followed by oral administration of FITC‑Dextran (MW 40000, 0.6 mg/g body weight). Four hours after gavage, mice were sacrificed, and plasma samples were collected for fluorescence intensity measurement at 490 nm. A standard curve of FD40000 was used to calculate the plasma concentration. Intestinal permeability was obviously impaired in Acss2^CKO mice, as determined by FD4 (FITC‑Dextran, MW 40000) gavage.

: FITC-Dextran (MW 40000) purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 Sep 17:326:123723. [Abstract]; The time-dependent variations of normalized fluorescence of FITC-Dextran (40, 70, 150 kDa; 0.5 mg/mL) and FITC-BSA in TNHs with Cp of 70 mg/mL. The inset shows confocal microscopic images of TNHs containing FITC-BSA at different time points after the photo-bleaching in FRAP experiments. The C_p is 70 mg/mL. Scale bar, 50 μm.

: FITC-Dextran (MW 40000) purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Dec 31.; The phagocytic activity was analyzed using FITC-Dextran (MW 40000) (1 mg/mL; 30 min) by flow cytometry. The values are presented as mean ± SD (n = 3). Significant differences with the control group (Ctrl) were designated as ⁎⁎⁎P < 0.001. The figure shown is representative of three independent experiments.

: FITC-Dextran (MW 40000) purchased from MedChemExpress. Usage Cited in: Food Biosci. 2025 Aug 30;72:107512.; FITC-Dextran (MW 40000) (1 mg/mL; 6 h) test results of the fluorescence intensity of the solution under the Caco-2 cell barrier. The experiment was repeated six times, n = 6; with “∗” representing P < 0.05; P < 0.05 is considered to indicate a statistically significant difference.

: FITC-Dextran (MW 40000) purchased from MedChemExpress. Usage Cited in: J Control Release. 2024 Nov:375:698-711. [Abstract]; Representative images of fluorescein isothiocyanate-conjugated dextran (FITC-Dextran (MW 40000), 100 μg; i.v.; single dose) distribution in normal and infected lung tissues with different treatments. Scale bar: 50 μm.

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Description	FITC-Dextran (MW 40000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 40000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 40000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption^[1]^[2]^[3].
In Vitro	Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of cells^[1]: For use with apoptotic cells 1. Incubate cells at 43.5°C for 1 hour and at 37°C for 8 hours to induce apoptosis. 2. Suspend the cells in 100 μL of medium, and mix in Q-prep tubes with 10 μL of propidium iodide (PI), 10 μL of FITC-Dextran (MW 40000) (the final concentration of PI and FITC-Dextran (MW 40000) is 7.5 μM and 1.13 μM, respectively). 3. Incubate cells for 25 min at room temperature in the dark. 4. Take the labeled cells with 3 mL of medium and centrifuge for 10 min at 500 g. 5. Take centrifuged cells with 1 mL of medium and use flow cytometry or fluorescence microscopy analyze (PI: Ex=500 nm, Em=600 nm; FITC-Dextran (MW 40000): Ex=495 nm, Em=525 nm). Paracellular permeability measurement^[4] 1. Add FITC-dextran (0.1 mg/mL) to the basal media in the transwell chamber. 2. Collect media from the transwell insert after 15 min. 3. Measure the fluorescence signal (Ex=485 nm, Em=538 nm). 4. Calculate FITC-dextran concentration based on fluorescence intensity. 5. Calculate permeability. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. FITC-Dextran (MW 40000) Related Antibodies
In Vivo	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). For intestinal barrier function assay^[5] 1. Fast mice for 4 h. 2. Orally gavage mice with FITC-Dextran MW 40000 (0.6 mg/g). 3. Measure fluorescence intensity of plasma in 4 h (excitation nm/emission 520 nm). MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight	40000(Average)
CAS No.	60842-46-8
Appearance	Solid
Color	Yellow to orange
Emission (Em)	525
Excitation (Ex)	488
SMILES	[FITC-Dextran (MW 40000)]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility	In Vitro: H₂O : 50 mg/mL (Need ultrasonic) Molarity Calculator Dilution Calculator Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Mass = Concentration × Volume × Molecular Weight * Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final) This equation is commonly abbreviated as: C₁V₁ = C₂V₂ Concentration _(start) C1 × Volume _(start) V1 = Concentration _(final) C2 × Volume _(final) V2 In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kg Animal weight (per animal) g Dosing volume (per animal) μL Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details. The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). Tel: 609-228-6898 E-mail: [email protected] Technical Support: [email protected]
Purity & Documentation	Select Batch: Data Sheet (281 KB) SDS (393 KB) COA (253 KB) Handling Instructions (2659 KB)
References	[1]. Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1). [2]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15. [Content Brief] [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189. [Content Brief] [4]. Okabayashi K, et al. Cdc42 activates paracellular transport in polarised submandibular gland cells. Arch Oral Biol. 2021 Dec;132:105276. [Content Brief] [5]. Yu W, et al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282. [Content Brief]