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  2. Nitro blue tetrazolium chloride

Nitro blue tetrazolium chloride (Synonyms: NBT)

Cat. No.: HY-15925 Purity: 99.82%
Handling Instructions

Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.

For research use only. We do not sell to patients.

Nitro blue tetrazolium chloride Chemical Structure

Nitro blue tetrazolium chloride Chemical Structure

CAS No. : 298-83-9

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10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
250 mg USD 60 In-stock
Estimated Time of Arrival: December 31
500 mg USD 72 In-stock
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Description

Nitro blue tetrazolium chloride (NBT) is a substrate for dehydrogenases; is used with the alkaline phosphatase substrate 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures.

Molecular Weight

817.64

Formula

C₄₀H₃₀Cl₂N₁₀O₆

CAS No.

298-83-9

SMILES

COC1=CC(C2=CC=C(N3N=C(C4=CC=CC=C4)N=[N+]3C5=CC=C([N+]([O-])=O)C=C5)C(OC)=C2)=CC=C1N6N=C(C7=CC=CC=C7)N=[N+]6C8=CC=C([N+]([O-])=O)C=C8.[Cl-].[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

70%DMF : ≥ 100 mg/mL (122.30 mM)

DMSO : 33.33 mg/mL (40.76 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2230 mL 6.1152 mL 12.2303 mL
5 mM 0.2446 mL 1.2230 mL 2.4461 mL
10 mM 0.1223 mL 0.6115 mL 1.2230 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (3.06 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.06 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.06 mM); Clear solution

*All of the co-solvents are provided by MCE.

Purity: 99.82%

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Keywords:

Nitro blue tetrazoliumNBTOthersInhibitorinhibitorinhibit

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Product name:
Nitro blue tetrazolium chloride
Cat. No.:
HY-15925
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