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  4. 2-NBDG

2-NBDG is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence. It has lower photostability than the rhodamine-based fluorescent probes.

For research use only. We do not sell to patients.

2-NBDG Chemical Structure

2-NBDG Chemical Structure

CAS No. : 186689-07-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in Water
ready for reconstitution
 USD 132 In-stock
Solution
10 mM * 1 mL in Water USD 132 In-stock
Solid
1 mg USD 48 In-stock
5 mg USD 120 In-stock
10 mg USD 170 In-stock
25 mg USD 340 In-stock
50 mg USD 533 In-stock
100 mg USD 800 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 33 publication(s) in Google Scholar

2-NBDG Related Antibodies

Top Publications Citing Use of Products

    2-NBDG purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Apr 19;2022:4608914.  [Abstract]

    H9c2 cells are incubated with 2-NBDG (50 μM; for 30 min). Flow cytometry quantified the 2-NBDG signals in HG-stimulated H9c2 cells.

    2-NBDG purchased from MedChemExpress. Usage Cited in: Food Funct. 2021 Mar 21;12(6):2726-2740.

    Glucose absorption and photomicrographs of HepG2 cells stained with 2-NBDG (100 μM; for 30 min).

    2-NBDG purchased from MedChemExpress. Usage Cited in: J Cell Physiol. 2021 Aug;236(8):5818-5831.  [Abstract]

    The cells are treated with 10 μM 2‐NBDG in serum‐free DMEM/F12 for 30 min.

    2-NBDG purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Jun 27;2021:5549047.

    Immunofluorescence images results of glucose uptake in glioblastoma cells determined by staining with 2-NBDG (50 μM; for 30 min) after treated with TGF-β1 in the absence or presence of GKT137831.

    2-NBDG purchased from MedChemExpress. Usage Cited in: Mar Drugs. 2020 Sep 22;18(9):485.  [Abstract]

    Caco-2 cells are treated with 100 μM 2‐NBDG for 30 min. Only 400 μg/mL of an AnF solution could significantly inhibit the transport of 2-NBDG in this Caco-2 monolayer cell model compared with the Control group, while the same concentration of LjF and KcF could not.

    2-NBDG purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Oct 5;2021:8542809.  [Abstract]

    3T3-L1 are incubated with or without media containing 10 nM insulin for 10 minutes. The media are changed to low-glucose DMEM containing 150 μg/mL 2-NBDG for 60 minutes at 37°C.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    2-NBDG is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence. It has lower photostability than the rhodamine-based fluorescent probes.

    In Vitro

    Preparation of 2-NBDG working solution
    1.1 Preparation of the stock solution
    Dissolve 1 mg of 2-NBDG in 2.92 mL of DDH2O to obtain 1 mM of 2-NBDG.
    Note: It is recommended to store the stock solution at -20 ℃ or -80 ℃ away from light and avoid repetitive freeze-thaw cycles.
    1.2 Preparation of 2-NBDG working solution.
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 10-200 μM of 2-NBDG working solution.
    Note: Please adjust the concentration of 2-NBDG working solution according to the actual situation.
    Cell staining
    2.1 For suspension cells: Centrifuge at 1,000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
    2.2 Add 1 mL of 2-NBDG working solution, and then incubate at room temperature for 5-60 minutes.
    2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
    2.4 Wash twice with PBS, 5 minutes each time.
    2.5 Resuspend cells with serum-free cell culture medium or PBS. .If test viability, recorded the optical density (O.D.) at 540/570 nm. Cell viability was calculated as a control ratio and plotted against the logarithmic concentration of the drug to calculate IC50.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    2-NBDG Related Antibodies
    Molecular Weight

    342.26

    Formula

    C12H14N4O8
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to orange

    Emission (Em)

    542
    Excitation (Ex)

    488
    SMILES

    O=C[C@H](NC1=CC=C([N+]([O-])=O)C2=NON=C21)[C@H]([C@@H]([C@@H](CO)O)O)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    H2O : 5 mg/mL (14.61 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9218 mL 14.6088 mL 29.2176 mL
    5 mM 0.5844 mL 2.9218 mL 5.8435 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 3.33 mg/mL (9.73 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.92%

    COA (252 KB) LCMS (263 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.9218 mL 14.6088 mL 29.2176 mL 73.0439 mL
    5 mM 0.5844 mL 2.9218 mL 5.8435 mL 14.6088 mL
    10 mM 0.2922 mL 1.4609 mL 2.9218 mL 7.3044 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    2-NBDG Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    2-NBDG186689-07-6Fluorescent DyeInhibitorinhibitorinhibit

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