2. Others Immunology/Inflammation
  3. Fluorescent Dye NO Synthase
  4. PYSNO

PYSNO is a lysosome-targeted fluorescent probe based on a pyridazinone skeleton (λem=515-565 nm, λex=405 nm) that can be used to track nitric oxide (NO) production in vivo. PYSNO exhibits a rapid, highly sensitive and highly selective "turn-on" response to endogenous and exogenous NO by blocking photoinduced electron transfer and regulating radiative decay rates. PYSNO enables precise in vivo monitoring in a mouse model of myocardial fibrosis and can be applied to the research of related diseases.

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PYSNO

PYSNO Chemical Structure

CAS No. : 2445485-84-5

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PYSNO Related Antibodies

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Description

PYSNO is a lysosome-targeted fluorescent probe based on a pyridazinone skeleton (λem=515-565 nm, λex=405 nm) that can be used to track nitric oxide (NO) production in vivo. PYSNO exhibits a rapid, highly sensitive and highly selective "turn-on" response to endogenous and exogenous NO by blocking photoinduced electron transfer and regulating radiative decay rates. PYSNO enables precise in vivo monitoring in a mouse model of myocardial fibrosis and can be applied to the research of related diseases[1][2].

In Vitro

In cell-free fluorescence spectroscopy assays, PYSNO (5 μM; 10 min, 60 s, 15 s) rapidly detects NO with a 23-fold fluorescence enhancement, a linear response range of 1 to 30 μM, and a limit of detection of 242 nM[1].
PYSNO (5 μM; 30 min) selectively accumulates in lysosomes of SH-SY5Y human neuroblastoma cells, as confirmed by a Pearson’s colocalization coefficient of 0.87 with Lyso-Tracker Red (HY-D1300) after NO-induced fluorescence activation[1].
PYSNO (5 μM; 30 min) detects exogenous NO in SH-SY5Y human neuroblastoma cells with dose-dependent fluorescence activation, showing a 10.5-fold enhancement at 200 μM NO[1].
PYSNO (5 μM; 30 min) detects endogenously produced NO in RAW264.7 mouse macrophages, showing a 2.8-fold fluorescence enhancement after NO induction, with signal reduction by NOS inhibition or NO scavenging[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PYSNO Related Antibodies
In Vivo

PYSNO (50 μM; i.v.; single dose) enables in vivo and ex vivo detection of nitric oxide production associated with myocardial fibrosis, showing significantly enhanced heart fluorescence in model mice relative to normal mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice (male, 10 weeks old; myocardial fibrosis induced by subcutaneous isoproterenol injection)[1]
Dosage: 50 μM (200 μL, 0.2% DMSO in PBS)
Administration: i.v. (tail vein); single dose
Result: Showed highly enhanced fluorescence in the heart region of myocardial fibrosis model mice relative to normal mice in in vivo imaging.
Confirmed significantly higher fluorescence intensity in myocardial fibrosis model mice relative to normal mice in ex vivo imaging of harvested hearts.
Molecular Weight

517.64

Formula

C28H31N5O3S
CAS No.
SMILES

NC1=C(C=C(C=C1)C2=CC=C(S2)N3N=C(C=CC3=O)C4=CC=C(C=C4)OCCCCN5CCOCC5)N
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Room temperature in continental US; may vary elsewhere.
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Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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PYSNO Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PYSNO2445485-84-5Fluorescent DyeNO SynthaseNitric oxide synthasesNOSanimal tissuemouse myocardial fibrosis modelslysosomesSH-SY5Y human neuroblastoma cellsnitric oxideRAW264.7 mouse macrophagesmyocardial fibrosisInhibitorinhibitorinhibit

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