D-Ala-Lys-AMCA hydrochloride

D-Ala-Lys-AMCA (25, 50 and 150 μM, 3 h) hydrochloride may be transported into liver cancer and Caco-2 cells^[1].
D-Ala-Lys-AMCA (25 μM, 10 min) hydrochloride can be uptaken by absorptive enterocytes of all small intestine segments, whereas there is a complete lack of fluorescence in colonic samples^[2].
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1].
1. Preparation of stock solution:
Use ddH2O or DMSO to prepare a D-Ala-Lys-AMCA stock solution.
2. Preparation of working solution:
Use preheated serum-free cell culture medium or PBS to dilute the stock solution to prepare a 1 mM D-Ala-Lys-AMCA working solution.
Note: Please adjust the concentration of D-Ala-Lys-AMCA working solution according to actual conditions and prepare it before use.
3. Cell staining:
3.1 Add PBS to wash the cells twice, 5 minutes each time.
3.2 Add D-Ala-Lys-AMCA working solution and incubate at 37°C for 1-2 h.
3.3 Aspirate the dye working solution, wash 2-3 times with culture medium, 5 minutes each time, and observe using a fluorescence microscope (Ex/Em = 390/480 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.