D-Ala-Lys-AMCA hydrochloride
Based on 1 publication(s) in Google Scholar
D-Ala-Lys-AMCA hydrochloride is a known proton-coupled oligopeptide transporter 1 (PEPT1) substrate that emits blue fluorescence. D-Ala-Lys-AMCA hydrochloride may be transported into liver cancer cells and Caco-2 cells based on fluorescence analysis. D-Ala-Lys-AMCA hydrochloride can be used for characterizing PEPT1-specific substrates or inhibitors (Ex/Em = 390/480 nm).
For research use only. We do not sell to patients.
- Purity: 98.60%
- CAS No.: 2703746-41-0
- Formula: C21H29ClN4O6
- Molecular Weight:468.93
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Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) D-Ala-Lys-AMCA hydrochloride
More
Biological Activity
D-Ala-Lys-AMCA (25, 50 and 150 μM, 3 h) hydrochloride may be transported into liver cancer and Caco-2 cells[1].
D-Ala-Lys-AMCA (25 μM, 10 min) hydrochloride can be uptaken by absorptive enterocytes of all small intestine segments, whereas there is a complete lack of fluorescence in colonic samples[2].
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Preparation of stock solution:
Use ddH2O or DMSO to prepare a D-Ala-Lys-AMCA stock solution.
2. Preparation of working solution:
Use preheated serum-free cell culture medium or PBS to dilute the stock solution to prepare a 1 mM D-Ala-Lys-AMCA working solution.
Note: Please adjust the concentration of D-Ala-Lys-AMCA working solution according to actual conditions and prepare it before use.
3. Cell staining:
3.1 Add PBS to wash the cells twice, 5 minutes each time.
3.2 Add D-Ala-Lys-AMCA working solution and incubate at 37°C for 1-2 h.
3.3 Aspirate the dye working solution, wash 2-3 times with culture medium, 5 minutes each time, and observe using a fluorescence microscope (Ex/Em = 390/480 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
480
390
Chemical Information
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CAS No. 2703746-41-0
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Appearance Solid
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Molecular Weight 468.93
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Formula C21H29ClN4O6
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Color Light yellow to yellow
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Toxicol Appl Pharmacol
Exploring the potential use of Caenorhabditis elegans as an animal model for evaluating chemical-induced intestinal dysfunction. [Abstract]2024 Dec:493:117140. PMID: 39500396
Solvent & Solubility
H2O : 100 mg/mL (213.25 mM; Need ultrasonic)
DMSO : 100 mg/mL (213.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Gong Y, et al. Specific expression of proton-coupled oligopeptide transporter 1 in primary hepatocarcinoma-anovel strategy for tumor-targeted therapy. Oncol Lett. 2017 Oct;14(4):4158-4166. [Content Brief]
[2]. Groneberg DA, et al. Intestinal peptide transport: ex vivo uptake studies and localization of peptide carrier PEPT1. Am J Physiol Gastrointest Liver Physiol. 2001 Sep;281(3):G697-704. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.1325 mL | 10.6626 mL | 21.3251 mL | 53.3129 mL |
| 5 mM | 0.4265 mL | 2.1325 mL | 4.2650 mL | 10.6626 mL | |
| 10 mM | 0.2133 mL | 1.0663 mL | 2.1325 mL | 5.3313 mL | |
| 15 mM | 0.1422 mL | 0.7108 mL | 1.4217 mL | 3.5542 mL | |
| 20 mM | 0.1066 mL | 0.5331 mL | 1.0663 mL | 2.6656 mL | |
| 25 mM | 0.0853 mL | 0.4265 mL | 0.8530 mL | 2.1325 mL | |
| 30 mM | 0.0711 mL | 0.3554 mL | 0.7108 mL | 1.7771 mL | |
| 40 mM | 0.0533 mL | 0.2666 mL | 0.5331 mL | 1.3328 mL | |
| 50 mM | 0.0427 mL | 0.2133 mL | 0.4265 mL | 1.0663 mL | |
| 60 mM | 0.0355 mL | 0.1777 mL | 0.3554 mL | 0.8885 mL | |
| 80 mM | 0.0267 mL | 0.1333 mL | 0.2666 mL | 0.6664 mL | |
| 100 mM | 0.0213 mL | 0.1066 mL | 0.2133 mL | 0.5331 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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