2. Academic Validation
  3. Molecular mechanism and inhibitory targets of dioscin in HepG2 cells

Molecular mechanism and inhibitory targets of dioscin in HepG2 cells

  • Food Chem Toxicol. 2018 Oct;120:143-154. doi: 10.1016/j.fct.2018.07.016.
Ying-Shuo Zhang 1,  Yi-Long Ma 2,  Kiran Thakur 3,  Sayed Sajid Hussain 4,  Jun Wang 5,  Qi Zhang 6,  Jian-Guo Zhang 7,  Zhao-Jun Wei 8
Affiliations

Affiliations

  • 1 School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China; Xuancheng Campus, Hefei University of Technology, Xuancheng, 242000, People's Republic of China. Electronic address: [email protected].
  • 2 School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China; Xuancheng Campus, Hefei University of Technology, Xuancheng, 242000, People's Republic of China. Electronic address: [email protected].
  • 3 School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 4 School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 5 School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 6 School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 7 School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 8 School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
PMID: 29990575 DOI: 10.1016/j.fct.2018.07.016
Abstract

Dioscin has been known for its anti-cancer activity; however, its detailed molecular mechanisms have not been studied so far. Herein, we evaluated the anti-cancer activity of dioscin for proliferation inhibition and Apoptosis in HepG2 Cancer cells. Initially, dioscin was purified and identified from Polygonatum sibiricum by HPLC, MS, and NMR analysis, respectively. Dioscin inhibited the cell multiplication at IC50 of 8.34 μM, altered the cell morphology, arrested the cell cycle in G2/M phase and led to considerable programmed cell death. Furthermore, it has efficiently promoted the mitochondrial pathway and death receptor pathway. The inhibition of Caspase-8 and Caspase-9 proteins in these pathways abolished the dioscin induced Apoptosis significantly; while dioscin inhibited the PI3K/Akt/mTOR pathway. Moreover, dioscin exposure led to enhanced intracellular ROS generation and the mRNA expression of JNK gene which emphasized their involvement in the Apoptosis process in HepG2 cells.

Keywords

Apoptosis; Cell cycle; Dioscin; Molecular mechanism; Polygonatum sibiricum.

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