2. Apoptosis
  3. Caspase Apoptosis
  4. Z-LEHD-FMK

Z-LEHD-FMK is a selective and irreversible inhibitor of caspase-9, protects against lethal reperfusion injury and attenuates apoptosis. Z-LEHD-FMK exhibits the neuroprotective effect in a rat model of spinal cord trauma.

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Custom Peptide Synthesis

CAS No. : 210345-04-3

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Based on 15 publication(s) in Google Scholar

Other Forms of Z-LEHD-FMK:

Z-LEHD-FMK Related Antibodies

Top Publications Citing Use of Products

    Z-LEHD-FMK purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Mar 25;44(4):115462.  [Abstract]

    Cell death analysis in TECs treated with PBS or cisplatin, in the presence of inhibitors for Casp9i (Z-LEHD-FMK , 20μM, 24 h), pan-apoptosis (Z-VAD-FMK), necrosis (Nec-1), and pyroptosis (Vx 765).

    Z-LEHD-FMK purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586.  [Abstract]

    HFFs stably expressing UL36WT (B), UL36S177T (C), or empty vector were infected with HCMVΔUL36 (MOI = 1) in the absence or presence of z-IETD-FMK (10 μM or 20 μM). At 12 h.p.i., cell lysates were subject to immunoblots with the indicated antibodies.

    Z-LEHD-FMK purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586.  [Abstract]

    HFFs were infected with HCMV or HCMVΔUL36 (MOI = 1) in the absence or presence of z-LEHD-FMK. At 0, 6, and 12 h.p.i., cell lysates were subject to immunoblots with the indicated antibodies.

    Z-LEHD-FMK purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586.  [Abstract]

    HFFs stably expressing UL36, UL36C131R, UL36S177T, or empty vector were infected with HCMVΔUL36 (MOI = 1) in presence of z-IETD-FMK. At 96 h.p.i., the mRNA levels of the indicated HCMV genes in HFFs were measured by qRT-PCR.

    Z-LEHD-FMK purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586.  [Abstract]

    HFFs stably expressing UL36, UL36C131R, UL36S177T, or empty vector were infected with HCMVΔUL36 (MOI = 1) in the presence of z-IETD-FMK. At 96 h.p.i., supernatants were harvested for measurements of the viral titers with standard TCID50 assays.

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    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

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    • Purity & Documentation

    • References

    • Customer Review

    Description

    Z-LEHD-FMK is a selective and irreversible inhibitor of caspase-9, protects against lethal reperfusion injury and attenuates apoptosis. Z-LEHD-FMK exhibits the neuroprotective effect in a rat model of spinal cord trauma[1][2][3].

    IC50 & Target[1]

    Caspase-9

     

    In Vitro

    Z-LEHD-FMK (20 μM; pretreated for 30 min) completely protects HCT116 and 293 cells from TRAIL-induced toxicity[1].
    Z-LEHD-FMK (20 μM ; 6 h) protects normal human hepatocytes from TRAIL-induced apoptosis[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Z-LEHD-FMK Related Antibodies

    Apoptosis Analysis[1]

    Cell Line: SW480, H460, HCT116 and 293 cells
    Concentration: 20 μM
    Incubation Time: Pretreated for 30 min
    Result: Protected HCT116 and 293 cells from TRAIL-induced apoptosis.

    Western Blot Analysis[1]

    Cell Line: HCT116, SW480 cells
    Concentration: 20 μM
    Incubation Time: 2 h
    Result: Protected procaspase 3 from cleavage in HCT116 cells but not in SW480 cells, especially at the 16-h time point.
    In Vivo

    Z-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar albino rats (250-350 g) with SCI[2]
    Dosage: 0.8 μmol/kg
    Administration: I.v. for 1 or 7 days
    Result: Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.
    Molecular Weight

    690.72

    Formula

    C32H43FN6O10
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    Sequence Shortening

    Z-LEHD-FMK
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (144.78 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4478 mL 7.2388 mL 14.4776 mL
    5 mM 0.2896 mL 1.4478 mL 2.8955 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Purity & Documentation

    Purity: 98.0%

    SDS (252 KB)

    COA (249 KB) HNMR (290 KB) MS (72 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.4478 mL 7.2388 mL 14.4776 mL 36.1941 mL
    5 mM 0.2896 mL 1.4478 mL 2.8955 mL 7.2388 mL
    10 mM 0.1448 mL 0.7239 mL 1.4478 mL 3.6194 mL
    15 mM 0.0965 mL 0.4826 mL 0.9652 mL 2.4129 mL
    20 mM 0.0724 mL 0.3619 mL 0.7239 mL 1.8097 mL
    25 mM 0.0579 mL 0.2896 mL 0.5791 mL 1.4478 mL
    30 mM 0.0483 mL 0.2413 mL 0.4826 mL 1.2065 mL
    40 mM 0.0362 mL 0.1810 mL 0.3619 mL 0.9049 mL
    50 mM 0.0290 mL 0.1448 mL 0.2896 mL 0.7239 mL
    60 mM 0.0241 mL 0.1206 mL 0.2413 mL 0.6032 mL
    80 mM 0.0181 mL 0.0905 mL 0.1810 mL 0.4524 mL
    100 mM 0.0145 mL 0.0724 mL 0.1448 mL 0.3619 mL
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    Z-LEHD-FMK Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Z-LEHD-FMK210345-04-3CaspaseApoptosiscaspase-9lethalreperfusioninjuryapoptosisneuroprotectiveInhibitorinhibitorinhibit

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