Z-LEHD-FMK
Based on 15 publication(s) in Google Scholar
Z-LEHD-FMK is a selective and irreversible inhibitor of caspase-9, protects against lethal reperfusion injury and attenuates apoptosis. Z-LEHD-FMK exhibits the neuroprotective effect in a rat model of spinal cord trauma.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 210345-04-3
- Formula: C32H43FN6O10
- Molecular Weight:690.72
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Z-LEHD-FMK
More- Sci Adv. 2023 Oct 6;9(40):eadi6586. [Abstract]
- Cell Rep. 2025 Mar 25;44(4):115462. [Abstract]
- Cell Mol Life Sci. 2025 Nov 19;82(1):408. [Abstract]
- Chem Biol Interact. 2025 Oct 22:420:111693. [Abstract]
- J Cell Mol Med. 2020 Jul;24(14):8151-8165. [Abstract]
- J Cell Mol Med. 2019 Apr;23(4):2489-2504. [Abstract]
- Virol Sin. 2024 May 30:S1995-820X(24)00078-6. [Abstract]
- Food Chem Toxicol. 2020 Dec;146:111843. [Abstract]
- Food Chem Toxicol. 2019 Oct:132:110655. [Abstract]
- Food Chem Toxicol. 2018 Oct:120:143-154. [Abstract]
- mSphere. 2024 May 17:e0023624. [Abstract]
- Infect Immun. 2025 Aug 12;93(8):e0015825. [Abstract]
- SSRN. 2025 Oct 15.
- Preprints. 2024 Aug 14.
- Oxid Med Cell Longev. 2022 Jun 26:2022:2912276. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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WB
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WB
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RT-PCR
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Microbiological Assay
All Caspase Isoforms
More
Biological Activity
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Caspase-9 |
Z-LEHD-FMK (20 μM; pretreated for 30 min) completely protects HCT116 and 293 cells from TRAIL-induced toxicity[1].
Z-LEHD-FMK (20 μM ; 6 h) protects normal human hepatocytes from TRAIL-induced apoptosis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SW480, H460, HCT116 and 293 cells
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Concentration:20 μM
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Incubation Time:Pretreated for 30 min
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Result:Protected HCT116 and 293 cells from TRAIL-induced apoptosis.
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Cell Line:HCT116, SW480 cells
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Concentration:20 μM
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Incubation Time:2 h
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Result:Protected procaspase 3 from cleavage in HCT116 cells but not in SW480 cells, especially at the 16-h time point.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Wistar albino rats (250-350 g) with SCI[2]
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Dosage:0.8 μmol/kg
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Administration:I.v. for 1 or 7 days
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Result:Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.
Chemical Information
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CAS No. 210345-04-3
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Appearance Solid
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Molecular Weight 690.72
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Formula C32H43FN6O10
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Color White to yellow
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Sequence Shortening
Z-LEHD-FMK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (15)
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Journal Impact Factor
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Most Recent
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Sci Adv
Human cytomegalovirus UL36 inhibits IRF3-dependent immune signaling to counterbalance its immunoenhancement as apoptotic inhibitor. [Abstract]2023 Oct 6;9(40):eadi6586. PMID: 37792941
Z-LEHD-FMK purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586. [Abstract]
HFFs stably expressing UL36WT (B), UL36S177T (C), or empty vector were infected with HCMVΔUL36 (MOI = 1) in the absence or presence of z-IETD-FMK (10 μM or 20 μM). At 12 h.p.i., cell lysates were subject to immunoblots with the indicated antibodies.
Z-LEHD-FMK purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586. [Abstract]
HFFs were infected with HCMV or HCMVΔUL36 (MOI = 1) in the absence or presence of z-LEHD-FMK. At 0, 6, and 12 h.p.i., cell lysates were subject to immunoblots with the indicated antibodies.
Z-LEHD-FMK purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586. [Abstract]
HFFs stably expressing UL36, UL36C131R, UL36S177T, or empty vector were infected with HCMVΔUL36 (MOI = 1) in presence of z-IETD-FMK. At 96 h.p.i., the mRNA levels of the indicated HCMV genes in HFFs were measured by qRT-PCR.
Z-LEHD-FMK purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586. [Abstract]
HFFs stably expressing UL36, UL36C131R, UL36S177T, or empty vector were infected with HCMVΔUL36 (MOI = 1) in the presence of z-IETD-FMK. At 96 h.p.i., supernatants were harvested for measurements of the viral titers with standard TCID50 assays.
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Cell Rep
The actin and microtubule network regulator WHAMM is identified as a key kidney disease risk gene. [Abstract]2025 Mar 25;44(4):115462. PMID: 40138314
Z-LEHD-FMK purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Mar 25;44(4):115462. [Abstract]
Cell death analysis in TECs treated with PBS or cisplatin, in the presence of inhibitors for Casp9i (Z-LEHD-FMK , 20μM, 24 h), pan-apoptosis (Z-VAD-FMK), necrosis (Nec-1), and pyroptosis (Vx 765).
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Cell Mol Life Sci
2025 Nov 19;82(1):408. PMID: 41258568 -
Chem Biol Interact
A novel non-nad-based PARP1 inhibitor, Japoflavone B, triggered Caspase-3/GSDME-mediated pyroptosis through ROS/p38/p53 pathway in NSCLC. [Abstract]2025 Oct 22:420:111693. PMID: 40754060 -
J Cell Mol Med
Hinokiflavone induces apoptosis via activating mitochondrial ROS/JNK/caspase pathway and inhibiting NF-κB activity in hepatocellular carcinoma. [Abstract]2020 Jul;24(14):8151-8165. PMID: 32519392 -
J Cell Mol Med
JS-K induces reactive oxygen species-dependent anti-cancer effects by targeting mitochondria respiratory chain complexes in gastric cancer. [Abstract]2019 Apr;23(4):2489-2504. PMID: 30672108 -
Virol Sin
Pseudorabies virus VHS protein abrogates interferon responses by blocking NF-κB and IRF3 nuclear translocation. [Abstract]2024 May 30:S1995-820X(24)00078-6. PMID: 38823782 -
Food Chem Toxicol
Apigenin 7-O-glucoside promotes cell apoptosis through the PTEN/PI3K/AKT pathway and inhibits cell migration in cervical cancer HeLa cells. [Abstract]2020 Dec;146:111843. PMID: 33152472 -
Food Chem Toxicol
Methyl protodioscin from Polygonatum sibiricum inhibits cervical cancer through cell cycle arrest and apoptosis induction. [Abstract]2019 Oct:132:110655. PMID: 31271762 -
Food Chem Toxicol
2018 Oct:120:143-154. PMID: 29990575 -
mSphere
Mammalian orthoreovirus capsid protein σ3 antagonizes RLR-mediated antiviral responses by degrading MAVS. [Abstract]2024 May 17:e0023624. PMID: 38757961 -
Infect Immun
Rickettsia heilongjiangensis suppresses RIPK1 kinase-mediated host cell death during the infection. [Abstract]2025 Aug 12;93(8):e0015825. PMID: 40607949 -
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Oxid Med Cell Longev
Smad7 Is Highly Expressed in Human Degenerative Discs and Participates in IL-1 β-Induced Apoptosis of Rat AF Cells via the Mitochondria Pathway. [Abstract]2022 Jun 26:2022:2912276. PMID: 35795857
Solvent & Solubility
DMSO : 100 mg/mL (144.78 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Ozoren N, et, al. The caspase 9 inhibitor Z-LEHD-FMK protects human liver cells while permitting death of cancer cells exposed to tumor necrosis factor-related apoptosis-inducing ligand. Cancer Res. 2000 Nov 15; 60(22): 6259-65. [Content Brief]
[2]. Colak A, et, al. Neuroprotection and functional recovery after application of the caspase-9 inhibitor z-LEHD-fmk in a rat model of traumatic spinal cord injury. J Neurosurg Spine. 2005 Mar; 2(3): 327-34. [Content Brief]
[3]. Mocanu MM, et, al. Caspase inhibition and limitation of myocardial infarct size: protection against lethal reperfusion injury. Br J Pharmacol. 2000 May; 130(2): 197-200. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.4478 mL | 7.2388 mL | 14.4776 mL | 36.1941 mL |
| 5 mM | 0.2896 mL | 1.4478 mL | 2.8955 mL | 7.2388 mL | |
| 10 mM | 0.1448 mL | 0.7239 mL | 1.4478 mL | 3.6194 mL | |
| 15 mM | 0.0965 mL | 0.4826 mL | 0.9652 mL | 2.4129 mL | |
| 20 mM | 0.0724 mL | 0.3619 mL | 0.7239 mL | 1.8097 mL | |
| 25 mM | 0.0579 mL | 0.2896 mL | 0.5791 mL | 1.4478 mL | |
| 30 mM | 0.0483 mL | 0.2413 mL | 0.4826 mL | 1.2065 mL | |
| 40 mM | 0.0362 mL | 0.1810 mL | 0.3619 mL | 0.9049 mL | |
| 50 mM | 0.0290 mL | 0.1448 mL | 0.2896 mL | 0.7239 mL | |
| 60 mM | 0.0241 mL | 0.1206 mL | 0.2413 mL | 0.6032 mL | |
| 80 mM | 0.0181 mL | 0.0905 mL | 0.1810 mL | 0.4524 mL | |
| 100 mM | 0.0145 mL | 0.0724 mL | 0.1448 mL | 0.3619 mL |
Z-LEHD-FMK Related Classifications
HY-P1010 Related Classifications
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