Z-WEHD-FMK
Based on 5 publication(s) in Google Scholar
Z-WEHD-FMK is a potent, cell-permeable and irreversible caspase-1/5 inhibitor. Z-WEHD-FMK also exhibits a robust inhibitory effect on cathepsin B activity (IC50=6 μM). Z-WEHD-FMK can be used to investigate cells for evidence of apoptosis.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 210345-00-9
- Formula: C37H42FN7O10
- Molecular Weight:763.77
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Metabolism/Protease Compound Library
HY-L012
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Anti-Cancer Compound Library
HY-L025
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Pyroptosis Compound Library
HY-L059
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Angiogenesis-Related Compound Library
HY-L088
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Peptide Library
HY-L105
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Protease Inhibitor Library
HY-L147
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Multi-Target Compound Library
HY-L176
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Peptide Compound Library
HY-L105S
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) Z-WEHD-FMK
MoreAll Caspase Isoforms
MoreAll Cathepsin Isoforms
More
Biological Activity
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Cathepsin B |
Caspase-1 |
Z-WEHD-FMK (80 μM; 9 hours) elicits a near-complete blockage of C. trachomatis-induced cleavage of golgin-84 and increases GM130 expression in cells[1].Z-WEHD-FMK (30?min before being exposed to?E. piscicida) effectively inhibits 0909I?E. piscicida?induced ZF4 cells cytotoxicity and pyroptotic morphology. And in addition, it also inhibits the cytotoxicity induced by cytosolic LPS delivery[2].Z-WEHD-FMK (20 μM;18-24 hours following Cr3+,Ni2+, and Co2+) significantly induces a decrease of 76% to 86% in IL-1β release with 200 to 400 ppm Cr3+, it also induces a decrease of 35% to 45% with 48 ppm Ni2+ or higher, Finally, this caspase-1 inhibitor induced a decrease with 6 ppm Co2+, down to a level below the detection threshold, and a decrease of 40% to 48% with 12 to 24 ppm Co2+ in bone marrow-derived macrophages (BMDM)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C. trachomatis- or mock-infected HeLa cells
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Concentration:80 μM
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Incubation Time:9 hours
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Result:Increased golgin-84 and GM130 expression.
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Cell Line:Mycoplasma free-ZF4 cells
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Concentration:
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Incubation Time:30 min before being exposed to E. piscicida
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Result:Inhibited ZF4 cells cytotoxicity and pyroptotic morphology.
Chemical Information
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CAS No. 210345-00-9
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Appearance Solid
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Molecular Weight 763.77
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Formula C37H42FN7O10
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Color Light yellow to yellow
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Synonyms
Z-WE(OMe)HD(OMe)-FMK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses. [Abstract]2025 Dec 15;10(1):406. PMID: 41392286 -
Nature
2026 Apr;652(8112):1362-1374. PMID: 42020739 -
Cell
2025 May 29;188(11):3013-3029.e19. PMID: 40252640 -
Atherosclerosis
Inhibition of vascular neointima hyperplasia by FGF21 associated with FGFR1/Syk/NLRP3 inflammasome pathway in diabetic mice. [Abstract]2019 Oct;289:132-142. PMID: 31513948 -
Solvent & Solubility
DMSO : 100 mg/mL (130.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.27 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.27 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (287 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Kamada S, et al. Caspase-4 and caspase-5, members of the ICE/CED-3 family of cysteine proteases, are CrmA-inhibitable proteases.Cell Death Differ. 1997 Aug;4(6):473-8. [Content Brief]
[2]. Yang D, et al. Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish.Nat Commun. 2018 Aug 3;9(1):3052. [Content Brief]
[3]. Ferko MA, et al. Effects of metal ions on caspase-1 activation and interleukin-1β release in murine bone marrow-derived macrophages.PLoS One. 2018 Aug 23;13(8):e0199936. [Content Brief]
[4]. Newman ZL, et al. CA-074Me protection against anthrax lethal toxin.Infect Immun. 2009 Oct;77(10):4327-36. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3093 mL | 6.5465 mL | 13.0929 mL | 32.7324 mL |
| 5 mM | 0.2619 mL | 1.3093 mL | 2.6186 mL | 6.5465 mL | |
| 10 mM | 0.1309 mL | 0.6546 mL | 1.3093 mL | 3.2732 mL | |
| 15 mM | 0.0873 mL | 0.4364 mL | 0.8729 mL | 2.1822 mL | |
| 20 mM | 0.0655 mL | 0.3273 mL | 0.6546 mL | 1.6366 mL | |
| 25 mM | 0.0524 mL | 0.2619 mL | 0.5237 mL | 1.3093 mL | |
| 30 mM | 0.0436 mL | 0.2182 mL | 0.4364 mL | 1.0911 mL | |
| 40 mM | 0.0327 mL | 0.1637 mL | 0.3273 mL | 0.8183 mL | |
| 50 mM | 0.0262 mL | 0.1309 mL | 0.2619 mL | 0.6546 mL | |
| 60 mM | 0.0218 mL | 0.1091 mL | 0.2182 mL | 0.5455 mL | |
| 80 mM | 0.0164 mL | 0.0818 mL | 0.1637 mL | 0.4092 mL | |
| 100 mM | 0.0131 mL | 0.0655 mL | 0.1309 mL | 0.3273 mL |
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