1. Apoptosis Metabolic Enzyme/Protease
  2. Caspase Cathepsin
  3. Z-WEHD-FMK

Z-WEHD-FMK  (Synonyms: Z-WE(OMe)HD(OMe)-FMK)

Cat. No.: HY-P0111 Purity: ≥98.0%
COA Handling Instructions

Z-WEHD-FMK is a potent, cell-permeable and irreversible caspase-1/5 inhibitor. Z-WEHD-FMK also exhibits a robust inhibitory effect on cathepsin B activity (IC50=6 μM). Z-WEHD-FMK can be used to investigate cells for evidence of apoptosis.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Z-WEHD-FMK Chemical Structure

Z-WEHD-FMK Chemical Structure

CAS No. : 210345-00-9

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1 mg USD 70 In-stock
5 mg USD 210 In-stock
10 mg USD 340 In-stock
25 mg USD 680 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Z-WEHD-FMK

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Z-WEHD-FMK is a potent, cell-permeable and irreversible caspase-1/5 inhibitor. Z-WEHD-FMK also exhibits a robust inhibitory effect on cathepsin B activity (IC50=6 μM). Z-WEHD-FMK can be used to investigate cells for evidence of apoptosis[1][2][4].

IC50 & Target[1][2]

Cathepsin B

 

Caspase-1

 

In Vitro

Z-WEHD-FMK (80 μM; 9 hours) elicits a near-complete blockage of C. trachomatis-induced cleavage of golgin-84 and increases GM130 expression in cells[1].
Z-WEHD-FMK (30 min before being exposed to E. piscicida) effectively inhibits 0909I E. piscicida induced ZF4 cells cytotoxicity and pyroptotic morphology. And in addition, it also inhibits the cytotoxicity induced by cytosolic LPS delivery[2].
Z-WEHD-FMK (20 μM;18-24 hours following Cr3+,Ni2+, and Co2+) significantly induces a decrease of 76% to 86% in IL-1β release with 200 to 400 ppm Cr3+, it also induces a decrease of 35% to 45% with 48 ppm Ni2+ or higher, Finally, this caspase-1 inhibitor induced a decrease with 6 ppm Co2+, down to a level below the detection threshold, and a decrease of 40% to 48% with 12 to 24 ppm Co2+ in bone marrow-derived macrophages (BMDM)[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: C. trachomatis- or mock-infected HeLa cells
Concentration: 80 μM
Incubation Time: 9 hours
Result: Increased golgin-84 and GM130 expression.

Cell Viability Assay[2]

Cell Line: Mycoplasma free-ZF4 cells
Concentration:
Incubation Time: 30 min before being exposed to E. piscicida
Result: Inhibited ZF4 cells cytotoxicity and pyroptotic morphology.
Molecular Weight

763.77

Formula

C37H42FN7O10

CAS No.
Appearance

Solid

Color

Light yellow to yellow

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (130.93 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3093 mL 6.5465 mL 13.0929 mL
5 mM 0.2619 mL 1.3093 mL 2.6186 mL
10 mM 0.1309 mL 0.6546 mL 1.3093 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.27 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.27 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (3.27 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation
References
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Z-WEHD-FMK Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Z-WEHD-FMK
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