1. Apoptosis
  2. Caspase

Z-DEVD-FMK (Synonyms: Caspase-3 Inhibitor)

Cat. No.: HY-12466 Purity: >98.0%
Handling Instructions

Z-DEVD-FMK is a specific and irreversible caspase-3 inhibitor with IC50 of 18 μM.

For research use only. We do not sell to patients.

Z-DEVD-FMK Chemical Structure

Z-DEVD-FMK Chemical Structure

CAS No. : 210344-95-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 263 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
1 mg USD 66 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 mg USD 179 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
10 mg USD 299 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
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Customer Review

    Z-DEVD-FMK purchased from MCE. Usage Cited in: Cell Mol Life Sci. 2018 Mar;75(6):1117-1132.

    Sertoli cells (SC) are pretreated with BAY11-7082 at 2 μM for 1 h, followed by the addition of MC-LR into the culture medium. Expression of p-p65 and MMP-8 is measured by western blotting.

    Z-DEVD-FMK purchased from MCE. Usage Cited in: Cell Mol Life Sci. 2018 Mar;75(6):1117-1132.

    Sertoli cells (SC) are pretreated with LY294002 at 20 μM for 1 h followed by a 24-h treatment with MC-LR. Expression levels of p-p65 and MMP-8 are detected by western blotting.

    Z-DEVD-FMK purchased from MCE. Usage Cited in: Cell Mol Life Sci. 2018 Mar;75(6):1117-1132.

    Sertoli cells (SC) are pretreated with the PD98059 for 1 h followed by a 24-h treatment with MC-LR. Expression levels of MMP-8, c-Jun, c-Fos, p-ERK, ERK, p-JNK, and JNK are determined by western blotting.

    Z-DEVD-FMK purchased from MCE. Usage Cited in: Cell Mol Life Sci. 2018 Mar;75(6):1117-1132.

    Sertoli cells (SC) are pretreated with the SP600125 for 1 h followed by a 24-h treatment with MC-LR. Expression levels of MMP-8, c-Jun, c-Fos, p-ERK, ERK, p-JNK, and JNK are determined by western blotting.

    Z-DEVD-FMK purchased from MCE. Usage Cited in: Apoptosis. 2016 Feb;21(2):130-42.

    (a) CPT-11 induces cell cycle arrest and apoptosis in RAW 264.7macrophages. a.Western blot analysis of cleaved caspase-3 and PARP levels. (b) CPT-11 induced cell cycle arrest and apoptosis in mouse peritoneal macrophages. Western blot analysis of cleaved caspase-3 and PARP levels in peritoneal macrophages isolated from vehicleand CPT-11-administered mice. (c) Caspase-3 inhibitor z-DEVD-fmk (ZD) attenuates CPT-11-induced loss of GATA6+ peritoneal macrophages in mice. Western blot analy
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Z-DEVD-FMK is a specific and irreversible caspase-3 inhibitor with IC50 of 18 μM.

    IC50 & Target[1]

    Caspase-3

    18 μM (IC50)

    In Vitro

    N27 cells are exposed to MPP+ in the absence or presence of 50 μM Z-DIPD-FMK or 100 μM Z-DEVD-FMK or 50 μM Z-LEHD-FMK and then caspase-9 and caspase-3 enzymatic activities are determined by enzymatic assay at 12 and 24 h following exposure, respectively. Exposure to 300 μM MPP+ for 24 h in N27 cells results in an approximately 2.5-fold increase in caspase-3 enzyme activity. MPP+-induced increases in caspase-3 enzyme activity are significantly blocked by 50 μM Z-DIPD-FMK, 100 μM Z-DEVD-FMK, and 50 μM Z-LEHD-FMK[1].

    In Vivo

    Early Z-DEVD-FMK (160 ng) treatment improves motor and cognitive function after traumatic CNS injury induced by severe controlled cortical impact (CCI) in the mouse[2]. Treatment with Z-DEVD-FMK (160 ng) significantly improves neurological outcome when compared with traumatized animals treated with DMSO vehicle (p<0.01)[3].

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 33.33 mg/mL (49.85 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4955 mL 7.4776 mL 14.9553 mL
    5 mM 0.2991 mL 1.4955 mL 2.9911 mL
    10 mM 0.1496 mL 0.7478 mL 1.4955 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      z-DEVD-fmk is dissolved in ethanol and freshly diluted in PBS containing 2 % Tween-80[4].

    References
    Cell Assay
    [1]

    N27 cells and primary mesencephalic neurons are exposed to either 10-100 μM 6-OHDA or 10-300 μM MPP+ in the presence or absence of 0.1-50 μM Z-DIPD-FMK or 0.1-100 μM Z-DEVD-FMK or 50 μM Z-IETD-FMK or Z-LEHD-FMK for the duration of the experiment. N27 cells are incubated with 100 μM 6-OHDA for 24 h or 300 μM MPP+ for 36 h in the presence or absence of 50 μM Z-DEVD-FMK and cell death is determined by MTT assay, which is widely used to assess cell viability. After treatment, the cells are incubated in serum-free medium containing 0.25 mg/mL MTT for 3 h at 37°C. Formation of formazan from tetrazolium is measured at 570 nm with a reference wavelength at 630 nm using a SpectraMax microplate reader[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2][3]

    Mice[2]
    Male C57Bl/6 mice (20-25 g) are used. For treatment with Z-DEVD-fmk or vehicle after CCI, mice are reanesthetized with isoflurane at various times after injury, placed in a stereotaxic apparatus, and the CCI wound is reopened for intracerebroventricular injection. Either Z-DEVD-FMK (160 ng in 2 μL DMSO), or DMSO vehicle is injected over a 5-minute period.
    Rats[3]
    Male Sprague Dawley rats (425±25 g) are used. DMSO (5 μL) vehicle or Z-DEVD-FMK (160 ng in 5 μL of DMSO) is administered at a controlled rate of 0.5 μL/min via an infusion pump at 30 min before and at 6 and 24 hr after TBI. At the designated time periods after injury, animals are decapitated under sodium pentobarbital anesthesia (100 mg/kg, i.p.), and the brains are removed rapidly and dissected. Sham-operated (control) animals received anesthesia and surgery but are not subjected to trauma. Tissue samples are collected 1, 4, 12, 24, and 72 hr after TBI. Samples are frozen on dry ice and kept at −85°C.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    668.66

    Formula

    C₃₀H₄₁FN₄O₁₂

    CAS No.

    210344-95-9

    SMILES

    O=C(N[[email protected]@H](C(C)C)C(N[[email protected]](C(CF)=O)CC(OC)=O)=O)[[email protected]](CCC(OC)=O)NC([[email protected]](CC(OC)=O)NC(OCC1=CC=CC=C1)=O)=O

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

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    Product Name:
    Z-DEVD-FMK
    Cat. No.:
    HY-12466
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    Cat. No.: HY-12466