Z-YVAD-FMK
Based on 23 publication(s) in Google Scholar
Z-YVAD-FMK is a cell-permeable caspase-1 and -4 inhibitor with anti-inflammatory and anti-tumor activities.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 210344-97-1
- Formula: C31H39FN4O9
- Molecular Weight:630.66
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Z-YVAD-FMK
More- Cell. 2025 May 29;188(11):3013-3029.e19. [Abstract]
- Adv Mater. 2025 Oct 17:e08245. [Abstract]
- Nat Biomed Eng. 2023 Mar;7(3):281-297. [Abstract]
- Int J Oral Sci. 2025 Feb 25;17(1):14. [Abstract]
- Sci Transl Med. 2023 Jan 11;15(678):eabl7895. [Abstract]
- Adv Sci (Weinh). 2025 Mar;12(12):e2409769. [Abstract]
- J Allergy Clin Immunol. 2022 Aug;150(2):337-351. [Abstract]
- J Hazard Mater. 2021 Jun 5:411:125134. [Abstract]
- Apoptosis. 2026 Feb 2;31(2):60. [Abstract]
- Clin Transl Med. 2025 Feb;15(2):e70221. [Abstract]
- Ecotoxicol Environ Saf. 2024 Mar 1:272:116004. [Abstract]
- Biochem Pharmacol. 2026 May 16:118078. [Abstract]
- Int Immunopharmacol. 2024 Apr 20:131:111785. [Abstract]
- Toxicology. 2024 Nov:508:153906. [Abstract]
- J Virol. 2022 May 25;96(10):e0030922. [Abstract]
- Diabetes Metab Syndr Obes. 2025 Jun 10:18:1903-1914. [Abstract]
- Hum Cell. 2022 May;35(3):803-816. [Abstract]
- Int J Med Microbiol. 2023 Sep 26;313(5):151586. [Abstract]
- Anticancer Drugs. 2025 Nov 5. [Abstract]
- SSRN. 2023 Nov 8.
- Research Square Print. January 3rd, 2023.
- Biomed Pharmacother. 2022 Jul;151:113098. [Abstract]
- Research Square Print. 2022 Jun.
All Caspase Isoforms
More
Biological Activity
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Caspase |
Z-YVAD-FMK (100 μM; 24 hours) significantly downregulated the growth inhibition induced by butyrate in Caco-2 cells[1].? Z-YVAD-FMK (20 μM; pre 1 hour; 24 hours) attenuates the apoptotic induction of III-10 on both HepG2 and BEL-7402 cells, the apoptotic rate of -10 on HepG2 cells is reduced by Z-VAD-FMK from 19.88% to 8.34%, while that on BEL-7402 cells is reduced from 17.56% to 11.98%[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Caco-2 cells
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Concentration:0-100 μM
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Incubation Time:24 hours
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Result:Inhibited Caco-2 cells growth.
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Cell Line:BEL-7402 and HepG2 cells
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Concentration:20 μM
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Incubation Time:Pre 1 hour; 24 hours
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Result:Induced a caspase-dependent apoptosis in cells.
Chemical Information
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CAS No. 210344-97-1
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Appearance Solid
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Molecular Weight 630.66
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Formula C31H39FN4O9
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Color White to off-white
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Sequence
Z-Tyr-Val-Ala-Asp-FMK
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Sequence Shortening
Z-YVAD-FMK
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (23)
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Journal Impact Factor
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Most Recent
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Cell
2025 May 29;188(11):3013-3029.e19. PMID: 40252640 -
Adv Mater
Ultrasound-Activated Sonophage Synergizes Sonodynamic Therapy and Saltoptosis for Solid Tumor Eradication. [Abstract]2025 Oct 17:e08245. PMID: 41104711 -
Nat Biomed Eng
Activatable near-infrared probes for the detection of specific populations of tumour-infiltrating leukocytes in vivo and in urine. [Abstract]2023 Mar;7(3):281-297. PMID: 36941352 -
Int J Oral Sci
Circadian disruption by simulated shift work aggravates periodontitis via orchestrating BMAL1 and GSDMD-mediated pyroptosis. [Abstract]2025 Feb 25;17(1):14. PMID: 40000642 -
Sci Transl Med
Targeting Src reactivates pyroptosis to reverse chemoresistance in lung and pancreatic cancer models. [Abstract]2023 Jan 11;15(678):eabl7895. PMID: 36630483 -
Adv Sci (Weinh)
Chromosome Missegregation Triggers Tumor Cell Pyroptosis and Enhances Anti-Tumor Immunotherapy in Colorectal Cancer. [Abstract]2025 Mar;12(12):e2409769. PMID: 39903759 -
J Allergy Clin Immunol
IL-17A mediates pyroptosis via the ERK pathway and contributes to steroid resistance in CRSwNP. [Abstract]2022 Aug;150(2):337-351. PMID: 35346673 -
J Hazard Mater
A tandem activation of NLRP3 inflammasome induced by copper oxide nanoparticles and dissolved copper ion in J774A.1 macrophage. [Abstract]2021 Jun 5:411:125134. PMID: 33485222 -
Apoptosis
CD95 ligand drives abdominal aortic aneurysm progression through Caspase-8-mediated GSDMD-dependent endothelial pyroptosis: modulation by SRC kinase. [Abstract]2026 Feb 2;31(2):60. PMID: 41622361 -
Clin Transl Med
Sodium pump subunit NKAα1 protects against diabetic endothelial dysfunction by inhibiting ferroptosis through the autophagy-lysosome degradation of ACSL4. [Abstract]2025 Feb;15(2):e70221. PMID: 39902679 -
Ecotoxicol Environ Saf
From inflammation to pyroptosis: Understanding the consequences of cadmium exposure in chicken liver cells. [Abstract]2024 Mar 1:272:116004. PMID: 38290315 -
Biochem Pharmacol
Sotorasib induces intestinal epithelial injury through suppression of the cAMP/PKA/CREB signaling axis. [Abstract]2026 May 16:118078. PMID: 42144204 -
Int Immunopharmacol
Dapagliflozin prevents kidney podocytes pyroptosis via miR-155-5p/HO-1/NLRP3 axis modulation. [Abstract]2024 Apr 20:131:111785. PMID: 38479158 -
Toxicology
Proteasome inhibition induces apoptosis through simultaneous inactivation of MCL-1/BCL-XL by NOXA independent of CHOP and JNK pathways. [Abstract]2024 Nov:508:153906. PMID: 39117261 -
J Virol
Peste Des Petits Ruminants Virus N Protein Is a Critical Proinflammation Factor That Promotes MyD88 and NLRP3 Complex Assembly. [Abstract]2022 May 25;96(10):e0030922. PMID: 35502911 -
Diabetes Metab Syndr Obes
Inhibition of Caspase-3/GSDME Pathway-Mediated Pyroptosis of Renal Tubular Epithelial Cells by Dagliflozin in the Pathogenesis of Diabetic Kidney Disease and Study of Its Mechanism. [Abstract]2025 Jun 10:18:1903-1914. PMID: 40524871 -
Hum Cell
A20 alleviated caspase-1-mediated pyroptosis and inflammation stimulated by Porphyromonas gingivalis lipopolysaccharide and nicotine through autophagy enhancement. [Abstract]2022 May;35(3):803-816. PMID: 35212946 -
Int J Med Microbiol
Polydatin alleviates mycoplasma pneumoniae-induced injury via inhibition of Caspase-1/GSDMD-dependent pyroptosis. [Abstract]2023 Sep 26;313(5):151586. PMID: 37776814 -
Anticancer Drugs
Activation of the caspase-1/gasdermin D pathway via α-linolenic acid-mediated GPR120 signaling induces pyroptosis and suppresses ovarian cancer tumor growth. [Abstract]2025 Nov 5. PMID: 41191783 -
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Biomed Pharmacother
Dendrobium officinale polysaccharides attenuate uropathogenic Escherichia coli (UPEC)-induced pyroptosis in macrophage cells. [Abstract]2022 Jul;151:113098. PMID: 35594714 -
Solvent & Solubility
DMSO : 125 mg/mL (198.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.30 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Li H1,et al.Aluminum hydroxide adjuvants activate caspase-1 and induce IL-1beta and IL-18 release.J Immunol. 2007 Apr 15;178(8):5271-6. [Content Brief]
[2]. Avivi-Green C, et al. Different molecular events account for butyrate-induced apoptosis in two human colon cancer cell lines.J Nutr. 2002 Jul;132(7):1812-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5856 mL | 7.9282 mL | 15.8564 mL | 39.6410 mL |
| 5 mM | 0.3171 mL | 1.5856 mL | 3.1713 mL | 7.9282 mL | |
| 10 mM | 0.1586 mL | 0.7928 mL | 1.5856 mL | 3.9641 mL | |
| 15 mM | 0.1057 mL | 0.5285 mL | 1.0571 mL | 2.6427 mL | |
| 20 mM | 0.0793 mL | 0.3964 mL | 0.7928 mL | 1.9821 mL | |
| 25 mM | 0.0634 mL | 0.3171 mL | 0.6343 mL | 1.5856 mL | |
| 30 mM | 0.0529 mL | 0.2643 mL | 0.5285 mL | 1.3214 mL | |
| 40 mM | 0.0396 mL | 0.1982 mL | 0.3964 mL | 0.9910 mL | |
| 50 mM | 0.0317 mL | 0.1586 mL | 0.3171 mL | 0.7928 mL | |
| 60 mM | 0.0264 mL | 0.1321 mL | 0.2643 mL | 0.6607 mL | |
| 80 mM | 0.0198 mL | 0.0991 mL | 0.1982 mL | 0.4955 mL | |
| 100 mM | 0.0159 mL | 0.0793 mL | 0.1586 mL | 0.3964 mL |
Z-YVAD-FMK Related Classifications
HY-P1009 Related Classifications
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