1. Apoptosis
  2. Caspase
  3. Z-YVAD-FMK

Z-YVAD-FMK 

Cat. No.: HY-P1009 Purity: >98.0%
Handling Instructions

AA-Z-YVAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor with anti-inflammatory and anti-tumor activities.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Z-YVAD-FMK Chemical Structure

Z-YVAD-FMK Chemical Structure

CAS No. : 210344-97-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 694 In-stock
Estimated Time of Arrival: December 31
1 mg USD 220 In-stock
Estimated Time of Arrival: December 31
5 mg USD 500 In-stock
Estimated Time of Arrival: December 31
10 mg USD 820 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

AA-Z-YVAD-FMK is a cell-permeable, irreversible pan-caspase inhibitor with anti-inflammatory and anti-tumor activities.

IC50 & Target[1]

Caspase

 

In Vitro

Z-YVAD-FMK (100 μM; 24 hours) significantly downregulated the growth inhibition induced by butyrate in Caco-2 cells[1].
Z-YVAD-FMK (20 μM; pre 1 hour; 24 hours) attenuates the apoptotic induction of III-10 on both HepG2 and BEL-7402 cells, the apoptotic rate of -10 on HepG2 cells is reduced by Z-VAD-FMK from 19.88% to 8.34%, while that on BEL-7402 cells is reduced from 17.56% to 11.98%[4].
Z-YVAD-FMK (1-10 μM; 24 hours) shows inhibitory effect in various cells against TNFr- or anti-CD95-induced cell death, exhibits IC50 values of 0.0015 μM (Murine hepatocytes), 0.027 μM (Murine hepatocytes), 4.8 μM (HepG2), 5.8 μM (HepG2), 1.6 μM (Hela) and 1.1 μM (Jurkat), respectively[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Caco-2 cells
Concentration: 0-100 μM
Incubation Time: 24 hours
Result: Inhibited Caco-2 cells growth.

Apoptosis Analysis[1]

Cell Line: BEL-7402 and HepG2 cells
Concentration: 20 μM
Incubation Time: Pre 1 hour; 24 hours
Result: Induced a caspase-dependent apoptosis in cells.
In Vivo

Z-VAD-FMK (intraperitoneal injection; 10mg/kg; pre- 30 minutes) significantly delayed preterm delivery at 18 hours, but after 36 hours treatment, non different exists between Z-VAD-FMK-pretreated and control groups[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Day 14.5 pregnant CD1 mice[3]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection; 10mg/kg; pre-30 minutes
Result: Prevented HK-GBS-induced preterm delivery.
Molecular Weight

630.66

Formula

C₃₁H₃₉FN₄O₉

CAS No.

210344-97-1

Sequence

Z-Tyr-Val-Ala-Asp-FMK

Sequence Shortening

Z-YVAD-FMK

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -80°C 2 years
-20°C 1 year
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (396.41 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5856 mL 7.9282 mL 15.8564 mL
5 mM 0.3171 mL 1.5856 mL 3.1713 mL
10 mM 0.1586 mL 0.7928 mL 1.5856 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.30 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.30 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (3.30 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: >98.0%

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Keywords:

Z-YVAD-FMKCaspaseAnti-inflammatoryanti-tumor,Inhibitorinhibitorinhibit

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