1. Apoptosis
  2. Caspase
    Apoptosis
  3. Z-LEHD-FMK TFA

Z-LEHD-FMK TFA 

Cat. No.: HY-P1010A
Handling Instructions

Z-LEHD-FMK TFA is a selective and irreversible inhibitor of caspase-9, protects against lethal reperfusion injury and attenuates apoptosis. Z-LEHD-FMK TFA exhibits the neuroprotective effect in a rat model of spinal cord trauma.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Z-LEHD-FMK TFA Chemical Structure

Z-LEHD-FMK TFA Chemical Structure

CAS No. : 524746-03-0

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Description

Z-LEHD-FMK TFA is a selective and irreversible inhibitor of caspase-9, protects against lethal reperfusion injury and attenuates apoptosis. Z-LEHD-FMK TFA exhibits the neuroprotective effect in a rat model of spinal cord trauma[1][2][3].

IC50 & Target[1]

Caspase-9

 

In Vitro

Z-LEHD-FMK (20 μM; pretreated for 30 min) completely protects HCT116 and 293 cells from TRAIL-induced toxicity[1].
Z-LEHD-FMK (20 μM ; 6 h) protects normal human hepatocytes from TRAIL-induced apoptosis[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[1]

Cell Line: SW480, H460, HCT116 and 293 cells
Concentration: 20 μM
Incubation Time: Pretreated for 30 min
Result: Protected HCT116 and 293 cells from TRAIL-induced apoptosis.

Western Blot Analysis[1]

Cell Line: HCT116, SW480 cells
Concentration: 20 μM
Incubation Time: 2 h
Result: Protected procaspase 3 from cleavage in HCT116 cells but not in SW480 cells, especially at the 16-h time point.
In Vivo

Z-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar albino rats (250-350 g) with SCI[2]
Dosage: 0.8 μmol/kg
Administration: I.v. for 1 or 7 days
Result: Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.
Molecular Weight

804.74

Formula

C₃₄H₄₄F₄N₆O₁₂

CAS No.

524746-03-0

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
References
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Keywords:

Z-LEHD-FMKCaspaseApoptosiscaspase-9lethalreperfusioninjuryapoptosisneuroprotectiveInhibitorinhibitorinhibit

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Z-LEHD-FMK TFA
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