Cat. No.: HY-P1010A

Z-LEHD-FMK TFA

15 Cited Publications

Customer Review

Based on 15 publication(s) in Google Scholar

Z-LEHD-FMK TFA is a selective and irreversible inhibitor of caspase-9, protects against lethal reperfusion injury and attenuates apoptosis. Z-LEHD-FMK TFA exhibits the neuroprotective effect in a rat model of spinal cord trauma.

For research use only. We do not sell to patients.

CAS No.: 524746-03-0
Formula: C₃₄H₄₄F₄N₆O₁₂
Molecular Weight:804.74

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Z-LEHD-FMK TFA Chemical Structure

CAS No.: 524746-03-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (15)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Caspase-9

All Caspase Isoforms :

In Vitro

Z-LEHD-FMK (20 μM; pretreated for 30 min) completely protects HCT116 and 293 cells from TRAIL-induced toxicity^[1].
Z-LEHD-FMK (20 μM ; 6 h) protects normal human hepatocytes from TRAIL-induced apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

SW480, H460, HCT116 and 293 cells
Concentration:

20 μM
Incubation Time:

Pretreated for 30 min
Result:

Protected HCT116 and 293 cells from TRAIL-induced apoptosis.

Western Blot Analysis^[1]

Cell Line:

HCT116, SW480 cells
Concentration:

20 μM
Incubation Time:

2 h
Result:

Protected procaspase 3 from cleavage in HCT116 cells but not in SW480 cells, especially at the 16-h time point.

In Vivo

Z-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Wistar albino rats (250-350 g) with SCI^[2]
Dosage:

0.8 μmol/kg
Administration:

I.v. for 1 or 7 days
Result:

Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.

Chemical Information

CAS No. 524746-03-0
Molecular Weight 804.74
Formula C₃₄H₄₄F₄N₆O₁₂
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (15)

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Journal Impact Factor

Sci Adv

Human cytomegalovirus UL36 inhibits IRF3-dependent immune signaling to counterbalance its immunoenhancement as apoptotic inhibitor. [Abstract]

2023 Oct 6;9(40):eadi6586. PMID: 37792941

Z-LEHD-FMK TFA purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586. [Abstract]

HFFs stably expressing UL36WT (B), UL36S177T (C), or empty vector were infected with HCMVΔUL36 (MOI = 1) in the absence or presence of z-IETD-FMK (10 μM or 20 μM). At 12 h.p.i., cell lysates were subject to immunoblots with the indicated antibodies.

Z-LEHD-FMK TFA purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586. [Abstract]

HFFs were infected with HCMV or HCMVΔUL36 (MOI = 1) in the absence or presence of z-LEHD-FMK. At 0, 6, and 12 h.p.i., cell lysates were subject to immunoblots with the indicated antibodies.

Z-LEHD-FMK TFA purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586. [Abstract]

HFFs stably expressing UL36, UL36C131R, UL36S177T, or empty vector were infected with HCMVΔUL36 (MOI = 1) in presence of z-IETD-FMK. At 96 h.p.i., the mRNA levels of the indicated HCMV genes in HFFs were measured by qRT-PCR.

Z-LEHD-FMK TFA purchased from MedChemExpress. Usage Cited in: Sci Adv. 2023 Oct 6;9(40):eadi6586. [Abstract]

HFFs stably expressing UL36, UL36C131R, UL36S177T, or empty vector were infected with HCMVΔUL36 (MOI = 1) in the presence of z-IETD-FMK. At 96 h.p.i., supernatants were harvested for measurements of the viral titers with standard TCID50 assays.
Cell Rep

The actin and microtubule network regulator WHAMM is identified as a key kidney disease risk gene. [Abstract]

2025 Mar 25;44(4):115462. PMID: 40138314

Z-LEHD-FMK TFA purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Mar 25;44(4):115462. [Abstract]

Cell death analysis in TECs treated with PBS or cisplatin, in the presence of inhibitors for Casp9i (Z-LEHD-FMK , 20μM, 24 h), pan-apoptosis (Z-VAD-FMK), necrosis (Nec-1), and pyroptosis (Vx 765).
Cell Mol Life Sci

Prolyl endopeptidase is a multifunctional host factor required for FMDV infection. [Abstract]

2025 Nov 19;82(1):408. PMID: 41258568
Chem Biol Interact

A novel non-nad-based PARP1 inhibitor, Japoflavone B, triggered Caspase-3/GSDME-mediated pyroptosis through ROS/p38/p53 pathway in NSCLC. [Abstract]

2025 Oct 22:420:111693. PMID: 40754060
J Cell Mol Med

Hinokiflavone induces apoptosis via activating mitochondrial ROS/JNK/caspase pathway and inhibiting NF-κB activity in hepatocellular carcinoma. [Abstract]

2020 Jul;24(14):8151-8165. PMID: 32519392
J Cell Mol Med

JS-K induces reactive oxygen species-dependent anti-cancer effects by targeting mitochondria respiratory chain complexes in gastric cancer. [Abstract]

2019 Apr;23(4):2489-2504. PMID: 30672108
Virol Sin

Pseudorabies virus VHS protein abrogates interferon responses by blocking NF-κB and IRF3 nuclear translocation. [Abstract]

2024 May 30:S1995-820X(24)00078-6. PMID: 38823782
Food Chem Toxicol

Apigenin 7-O-glucoside promotes cell apoptosis through the PTEN/PI3K/AKT pathway and inhibits cell migration in cervical cancer HeLa cells. [Abstract]

2020 Dec;146:111843. PMID: 33152472
Food Chem Toxicol

Methyl protodioscin from Polygonatum sibiricum inhibits cervical cancer through cell cycle arrest and apoptosis induction. [Abstract]

2019 Oct:132:110655. PMID: 31271762
Food Chem Toxicol

Molecular mechanism and inhibitory targets of dioscin in HepG2 cells. [Abstract]

2018 Oct:120:143-154. PMID: 29990575
mSphere

Mammalian orthoreovirus capsid protein σ3 antagonizes RLR-mediated antiviral responses by degrading MAVS. [Abstract]

2024 May 17:e0023624. PMID: 38757961
Infect Immun

Rickettsia heilongjiangensis suppresses RIPK1 kinase-mediated host cell death during the infection. [Abstract]

2025 Aug 12;93(8):e0015825. PMID: 40607949
SSRN

2025 Oct 15.
Preprints

2024 Aug 14.
Oxid Med Cell Longev

Smad7 Is Highly Expressed in Human Degenerative Discs and Participates in IL-1 β-Induced Apoptosis of Rat AF Cells via the Mitochondria Pathway. [Abstract]

2022 Jun 26:2022:2912276. PMID: 35795857

Solvent & Solubility

In Vitro

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (263 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ozoren N, et, al. The caspase 9 inhibitor Z-LEHD-FMK protects human liver cells while permitting death of cancer cells exposed to tumor necrosis factor-related apoptosis-inducing ligand. Cancer Res. 2000 Nov 15; 60(22): 6259-65. [Content Brief]

[2]. Colak A, et, al. Neuroprotection and functional recovery after application of the caspase-9 inhibitor z-LEHD-fmk in a rat model of traumatic spinal cord injury. J Neurosurg Spine. 2005 Mar; 2(3): 327-34. [Content Brief]

[3]. Mocanu MM, et, al. Caspase inhibition and limitation of myocardial infarct size: protection against lethal reperfusion injury. Br J Pharmacol. 2000 May; 130(2): 197-200. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Z-LEHD-FMK TFA

15 Cited Publications

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Z-LEHD-FMK TFA

Publications Citing Use of MedChemExpress (MCE) Z-LEHD-FMK TFA

All Caspase Isoforms

Biological Activity

Chemical Information

Publications (15)

Solvent & Solubility

Purity & Documentation

References

Calculators

Help & FAQs

Z-LEHD-FMK TFA Related Classifications

HY-P1010A Related Classifications