Methyl protodioscin from Polygonatum sibiricum inhibits cervical cancer through cell cycle arrest and apoptosis induction

  • Food Chem Toxicol. 2019 Oct;132:110655. doi: 10.1016/j.fct.2019.110655.
Yi-Long Ma  1 ,  Ying-Shuo Zhang  2 ,  Fan Zhang  3 ,  Yuan-Yuan Zhang  4 ,  Kiran Thakur  5 ,  Jian-Guo Zhang  6 ,  Zhao-Jun Wei  7
Affiliations
  • 1. School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 2. School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 3. School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 4. School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 5. School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 6. School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China. Electronic address: [email protected].
  • 7. School of Food Science and Biological Engineering, Hefei University of Technology, Hefei, 230009, People's Republic of China; Anhui Province Key Laboratory of Functional Compound Seasoning, Anhui Qiangwang Seasoning Food Co., Ltd, Jieshou, 236500, People's Republic of China. Electronic address: [email protected].
PMID: 31271762 DOI: 10.1016/j.fct.2019.110655
Abstract

Methyl protodioscin (MPD) is a steroid saponin which has been well known for its pharmacological properties. Herein, we evaluated the anti-cancer activity of MPD for proliferation inhibition and Apoptosis induction in Hela cells. MPD was purified from the rhizoma of Polygonatum sibiricum primarily and identified by HPLC, UPLC-TOF-MS/MS and NMR analysis, respectively. Results showed that MPD repressed cell proliferation at IC50 of 18.49 μM, altered cell morphology, arrested the cell cycle in G2/M phase, facilitated the generation of intracellular ROS and led to cell Apoptosis in a concentration-dependent manner. Furthermore, MPD treatment promoted death receptor pathway and mitochondrial pathway efficiently. The inhibition of Caspase-8 and Caspase-9 proteins in these pathways abolished the Apoptosis significantly, further demonstrated the mechanism of MPD-induced Apoptosis. These findings offer novel information that MPD may be considered as a possible natural anti-cancerous agent in the form of functional foods or medicinal products.

Keywords
Apoptosis; Cell cycle; Methyl protodioscin; Molecular mechanism; Polygonatum sibiricum.
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