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Necrostatin-1

(Synonyms: Nec-1)
514 Cited Publications
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Based on 514 publication(s) in Google Scholar

Necrostatin-1 (Nec-1) is a potent and cross the blood-brain barrier necroptosis inhibitor with an EC50 of 490 nM in Jurkat cells. Necrostatin-1 inhibits RIP1 kinase (EC50=182 nM). Necrostatin-1 is also an IDO inhibitor.

For research use only. We do not sell to patients.

  • Purity: 99.89%
  • CAS No.: 4311-88-0
  • Formula: C13H13N3OS
  • Molecular Weight:259.33
  • Storage:
    Powder   -20°C, 3 years , 4°C, 2 years ; In solvent   -80°C, 1 year , -20°C, 6 months
  • Necrostatin-1 Chemical Structure
    Necrostatin-1 Chemical Structure
  • Experimental Validation Results for Necrostatin-1
  • Experimental Validation Results for Necrostatin-1
  • Experimental Validation Results for Necrostatin-1
  • Experimental Validation Results for Necrostatin-1
  • Experimental Validation Results for Necrostatin-1
  • Experimental Validation Results for Necrostatin-1
  • Experimental Validation Results for Necrostatin-1
  • Experimental Validation Results for Necrostatin-1
  • Experimental Validation Results for Necrostatin-1
  • Experimental Validation Results for Necrostatin-1
CAS No.: 4311-88-0
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Cell Proliferation/Viability AssayWB
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  • Cell Proliferation/Viability Assay
  • Cell Proliferation/Viability Assay
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All RIP kinase Isoforms

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All Indoleamine 2,3-Dioxygenase (IDO) Isoforms

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  • Necrostatin-1 Chemical Structure
    Necrostatin-1 Chemical Structure
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    Necrostatin-1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Jul;21(7):1473-1491. [Abstract]

    Customer Validation
     Necrostatin-1 (Nec-1, 10 μM) rescues the loss of neuronal cell viability induced by Psmd14 knockdown.

  • 1/16

    Necrostatin-1 purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Aug 27;12(1):47. [Abstract]

    Customer Validation
     Cell death induced by HOXC10 inhibition plus TTI-101 cotreatment was rescued by ferroptosis inhibitors. Cells with or without knockdown of HOXC10 were pretreated with HOXC10 TTI-101 for 12 h and then treated the indicated cell death inhibitors (3-MA-1 mmol/L, Necrostatin-1-20 μmol/L, Ferrostatin-1-2 μmol/L, Z-VAD-FMK-10 μmol/L, Glutathione-1 mmol/L, Deferoxamine (DFO-100 μmol/L)) for an additional 72 h. Effect on ferroptosis inhibitors rescuing cell death was assessed by cell viability assays.

  • 1/16

    Necrostatin-1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jun;11(21):e2309002. [Abstract]

    Customer Validation
     Necrostatin-1 (Nec-1; 25 μM; 24 h) significantly restores cell viability in THBS1-knockdown HTR8/SVneo cells.

  • 1/16

    Necrostatin-1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jun;11(21):e2309002. [Abstract]

    Customer Validation
     Necrostatin-1 (Nec-1; 25 μM; 24 h) significantly restores proliferative capacity in THBS1-knockdown HTR8/SVneo cells.

  • 1/16

    Necrostatin-1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jun;11(21):e2309002. [Abstract]

    Customer Validation
     Necrostatin-1 (Nec-1; 25 μM; 24 h) significantly reduces protein expression of RIPK1, p-RIPK3, RIPK3, p-MLKL, and DAMP molecules in THBS1-knockdown cells.

  • 1/16

    Necrostatin-1 purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2023 May 14.

    Customer Validation
     The viability of MC38 cells treated with PAD@MS for 48 h in the presence or absence of 10 µM Ferr-1 (Ferrostatin-1), 20 µM Nec-1 (Necrostatin-1), 10 µM Z-VAD-FMK, and 1 mM NAC.

  • 1/16

    Necrostatin-1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2022 Apr;18(4):726-744. [Abstract]

    Customer Validation
     Necrostatin-1 (Nec-1, 30 µM; 6.5 h) blocks TSZ-induced increases in p-MLKL, SQSTM1, and LC3-II levels in L929 cells.

  • 1/16

    Necrostatin-1 purchased from MedChemExpress. Usage Cited in: Braz J Med Biol Res. 2018 Nov 23;52(1):e7844. [Abstract]

    Customer Validation
     Representative western blot graphs showing the effects of tumor necrosis factor-α (TNF-α) on the protein expression levels of specific marker proteins receptor interacting protein kinase (RIPK3), mixed lineage kinase domain-like protein (MLKL) and p-MLKL, caspase 3 and cleaved caspase 3, and b-actin in MC3T3-E1 cells with TNF-α, Nec-1 or Z-IETD-FMK treatment.

  • 1/16

    Necrostatin-1 purchased from MedChemExpress. Usage Cited in: Exp Neurol. 2017 Sep:295:116-124. [Abstract]

    Customer Validation
     Nec-1 treatment inhibits RIP1-RIP3, p-DRP1 activation and reduces the expression of NLRP3 and cleaved caspase-1 at 24 h after SAH. Representative Western blots showing levels of RIP1, RIP3, p-DRP1, DRP1, NLRP3 and cleaved caspase-1.

  • 1/16

    Necrostatin-1 purchased from MedChemExpress. Usage Cited in: Sci Rep. 2017 Nov 23;7(1):16111. [Abstract]

    Customer Validation
     RIP1 does not mediate TNFα-induced apoptosis in RIP3 knockdown L929 cells. Nec-1 does not block the TNFα-induced death of RIP3 knockdown L929 cells. The cells are infected with the RIP3 shRNA or the negative control shRNA lentivirus, and western blotting is performed to determine RIP3 knockdown efficiency.

  • Necrostatin-1