1. PI3K/Akt/mTOR
    Autophagy
    Metabolic Enzyme/Protease
  2. PI3K
    Autophagy
    Mitophagy
    Endogenous Metabolite
  3. 3-Methyladenine

3-Methyladenine (Synonyms: 3-MA)

Cat. No.: HY-19312 Purity: 99.84%
Handling Instructions

3-Methyladenine is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K.

For research use only. We do not sell to patients.

3-Methyladenine Chemical Structure

3-Methyladenine Chemical Structure

CAS No. : 5142-23-4

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Customer Review

Based on 82 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE 3-Methyladenine

    3-Methyladenine purchased from MCE. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2018 Feb 1;50(2):144-155.

    Autophagy is involved in Rg1-inhibited apoptosis in macrophages Macrophages with serum deprivation are treated with 50 μM Rg1 for 48 h in the absence or presence of 3-MA (5 mM). The protein expression level of cleaved caspase-3 determined by western blot analysis in Raw264.7 macrophages.

    3-Methyladenine purchased from MCE. Usage Cited in: Oncotarget. 2017 Nov 22;8(65):109135-109150.

    Cell morphology of MCF-7 treated with RA (20 μM)+ω-3 PUFAs (80 μM) with or without 3-MA (5 mM) for 24h.

    3-Methyladenine purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2018;48(6):2318-2336.

    Cells are pretreated with 5 mM 3-MA or 2 μM for 1 h and then exposed to 40 μM EPA and 10 μM Rp for 48 h. Cell extracts are prepared and subjected to western blot analysis.

    3-Methyladenine purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96.

    The hepatic stellate cells (HSCs) activation is down-regulated along with autophagy following DMKG or 3MA treatment in vitro. Western blot analysis of LC3B, Beclin-1, α-SMA, and collagen-I expression from lysates of HSC-T6 incubated with 4mM DMKG or 10 mM 3MA for 12 h.

    3-Methyladenine purchased from MCE. Usage Cited in: J Dermatol Sci. 2018 Jan;89(1):11-18.

    Inhibitory effects of LED 585 nm on melanogenesis is reversed by 3-MA.

    3-Methyladenine purchased from MCE. Usage Cited in: J Dermatol Sci. 2018 Jan;89(1):11-18.

    Inhibitory effects of LED 585 nm on melanogenesis is reversed by 3-MA. Autophagy is decreased in 3-MA-treated group, which is reflected by the decrease in the ratio of LC3 II/LC3 I and increase in p62 protein

    3-Methyladenine purchased from MCE. Usage Cited in: Int J Mol Med. 2018 Jun;41(6):3221-3230.

    Western blot analysis is used to measure LC3, p62 and Beclin‑1 expression following the treatment of H9C2 cells with si‑miRNA‑30e and 3‑MA.

    3-Methyladenine purchased from MCE. Usage Cited in: Int J Oncol. 2018 Sep;53(3):1363-1373.

    The protein expression levels of LC3 and Beclin-1 proteins in HCT-116 cells. Cells are treated with 100 μg/mL active fraction of clove (AFC) for 48 h with or without pretreatment of 2 mM 3-MA or 1 nM Baflomycin A1 (BA) for 1 h.

    3-Methyladenine purchased from MCE. Usage Cited in: J Cell Mol Med. 2018 Oct;22(10):5132-5144.

    Melatonin regulation of mitophagy activity in the diabetic myocardium, Protein expression with representative gel blots of LC3 I/II, GAPDH (loading control).

    3-Methyladenine purchased from MCE. Usage Cited in: Biomed Res. 2018;39(2):87-94.

    Accumulation of LC3B II and p62 proteins in the livers of chloroform-treated mice is reduced by 3-MA treatment.

    3-Methyladenine purchased from MCE. Usage Cited in: PLoS One. 2018 Aug 6;13(8):e0201920.

    The pretreatment of Z-VAD-FMK significantly reduces the levels of cleaved caspase-3 and cleaved PARP. The pretreatment of 3-MA blocks the conversion of LC3-I to LC3-II.

    3-Methyladenine purchased from MCE. Usage Cited in: J Immunol Res. 2018 Dec 24;2018:9807139.

    Western blot assays show that 3-MA abrogates the Alda-1 induced expression increases in LC3BⅡ and Bcl2 and decreases in P62 and Bax.

    3-Methyladenine purchased from MCE. Usage Cited in: Int J Mol Med. 2018 May;41(5):2535-2544.

    MC3T3-E1 cells are treated with AICAR (10 μM) in the presence or absence of 3-MA (5 mM) or CQ (10 μM) for 24 h, after which western blot analysis is conducted.

    3-Methyladenine purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Mar;120(3):4366-4374.

    Representative Western blots show LC3 I/II and p62 protein expression in kidneys of vehicle group and 3-MA group mice at 72 hours after the Cisplatin treatment.

    3-Methyladenine purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119.

    Western blotting image protein levels of GRP78/Bip in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

    3-Methyladenine purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119.

    Western blotting image protein levels of LC3-I & LC3-II in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

    3-Methyladenine purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119.

    Western blotting image protein levels of p62 in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

    3-Methyladenine purchased from MCE. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534.

    CRC cells are treated with vehicle, BFA (100 nM), 3-MA (10 mM), or in combination for 24 h. Immunoblot analysis is used to detect LC3 turnover.

    3-Methyladenine purchased from MCE. Usage Cited in: Brain Behav Immun. 2019 Jul;79:244-255.

    Immunoblot analysis of NLRP3 in LPS-primed macrophages treated with KA (10 μM) or concurrently with autophagy inhibitor (3-MA, 5 mM). Statistical analysis of NLRP3 intensity during 3-MA or zaprinast treatment is shown.

    3-Methyladenine purchased from MCE. Usage Cited in: Front Endocrinol (Lausanne). 2019 Jun 17;10:370.

    LC3 II expression is regulated in CQ- or 3-MA-treated GnRH-OE Panc1 cells.
    • Biological Activity

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    • References

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    Description

    3-Methyladenine is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K.

    IC50 & Target[1]

    PtdIns3Kγ

    60 μM (IC50, Cell Assay)

    Vps34

    25 μM (IC50, Cell Assay)

    Autophagy

     

    Mitophagy

     

    Human Endogenous Metabolite

     

    In Vitro

    3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner[2].
    3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions[2].
    3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy[2].
    3-Methyladenine induces cell death is independent of autophagy inhibition[2].
    3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest[2].

    Cell Viability Assay[2]

    Cell Line: HeLa cells
    Concentration: 0 mM, 2.5 mM or 5 mM , 10 mM
    Incubation Time: 0 hour, 24 hours and 48 hours
    Result: Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation.

    Cell Autophagy Assay[2]

    Cell Line: HeLa cells
    Concentration: 5 mM
    Incubation Time: 24 hours
    Result: Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions.

    Western Blot Analysis[2]

    Cell Line: HeLa cells
    Concentration: 5 mM
    Incubation Time: 0 hour, 12 hours, 24 hours and 48 hours
    Result: Suppressed conversion of LC3-I to LC3-II between 12hours and 48 hours.
    In Vivo

    3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours[3].
    3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP[3].
    3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP[3].

    Animal Model: 10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290 g)[3]
    Dosage: 1.5 mg/100 g (1000 μM)
    Administration: Intraperitoneal injection
    Result: Alleviated Sodium Taurocholate-Induced SAP.
    Molecular Weight

    149.15

    Formula

    C₆H₇N₅

    CAS No.

    5142-23-4

    SMILES

    NC1=C2N=CN=C2N(C)C=N1

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 22.14 mg/mL (148.44 mM; Need ultrasonic)

    H2O : 4 mg/mL (26.82 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.7047 mL 33.5233 mL 67.0466 mL
    5 mM 1.3409 mL 6.7047 mL 13.4093 mL
    10 mM 0.6705 mL 3.3523 mL 6.7047 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.21 mg/mL (14.82 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.21 mg/mL (14.82 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.21 mg/mL (14.82 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.84%

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    Product Name:
    3-Methyladenine
    Cat. No.:
    HY-19312
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