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3-Methyladenine

(Synonyms: 3-MA)
1264 Cited Publications
Customer Review

Based on 1264 publication(s) in Google Scholar

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K.

For research use only. We do not sell to patients.

  • Purity: 99.91%
  • CAS No.: 5142-23-4
  • Formula: C6H7N5
  • Molecular Weight:149.15
  • Storage:
    Powder   -20°C, 3 years , 4°C, 2 years ; In solvent   -80°C, 6 months , -20°C, 1 month
  • 3-Methyladenine Chemical Structure
    3-Methyladenine Chemical Structure
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
  • Experimental Validation Results for 3-Methyladenine
CAS No.: 5142-23-4
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  • 3-Methyladenine Chemical Structure
    3-Methyladenine Chemical Structure
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    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Sep 26;9(1):254. [Abstract]

    Customer Validation
     N2a cells expressing Cadm4 or Cadm4-C347A were incubated with or without CHX, CHQ (Chloroquine, 50 µM), 3-MA (Methyladenine, 3 mM) or Mg132 (25 µM), and subjected for WB analysis

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Aug 27;12(1):47. [Abstract]

    Customer Validation
     Cell death induced by HOXC10 inhibition plus TTI-101 cotreatment was rescued by ferroptosis inhibitors. Cells with or without knockdown of HOXC10 were pretreated with HOXC10 TTI-101 for 12 h and then treated the indicated cell death inhibitors (3-MA-1 mmol/L, Necrostatin-1-20 μmol/L, Ferrostatin-1-2 μmol/L, Z-VAD-FMK-10 μmol/L, Glutathione-1 mmol/L, Deferoxamine (DFO-100 μmol/L)) for an additional 72 h. Effect on ferroptosis inhibitors rescuing cell death was assessed by cell viability assays.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2024 Dec:197:107046. [Abstract]

    Customer Validation
     BMDMs pretreated with degarelix (1 μM), aminoguanidine hydrochloride (AGHS, 100 μM), BafA1 (100 nM), NAC (5 mM), Z-VAD (20 μM), or 3-MA (5 mM) were infected with H37Rv (MOI = 2). At different time points after infection, bacterial survival was calculated by dividing the CFU at 24 h by the CFU at 3 h.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Feb 28;21(1):69. [Abstract]

    Customer Validation
     The autophagy is inhibited by 3-Methyladenine (3-MA; 5 mM; 24 h), as evidenced by decreased Atg5 expression and the ratio of LC3-II/LC3-I and increased p62 protein level in MLE-12 cells (Fig. B, C).

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2023 Feb 28;21(1):69. [Abstract]

    Customer Validation
     3-Methyladenine (3-MA; 5 mM; 24 h) markedly decreases the SWCNTs-induced increase in LC3B puncta, in MLE-12 cells.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2022 Dec 9;7(1):388. [Abstract]

    Customer Validation
     Cell proliferation determined by colony formation assay in A2780CP cells (10,000 cells) after CRL4CUL4A/DDB1 knockdown with or without 1 mM of 3-MA treatment for 36 h.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2022 Feb 14;9(1):9. [Abstract]

    Customer Validation
     GL261 and U251 cells were pretreated with 3-MA (5 mmol/L) for 1 h and then exposed to UTMD (ultrasonic intensity of 1.2 W/cm2 combined with 200 μl/ml MBs at a duty cycle of 10% for 60 s) followed by IR (2 Gy) exposure. Total cell lysates were immunoblotted with anti-p62, anti-LC3B, and anti-ACTB antibodies. The bar graphs show the quantification of LC3B2 and p62.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Dec 9:24:26-36. [Abstract]

    Customer Validation
     Cell viability assays of ZR-75-1 cells treated with PBS, MOF, αM, αMM@PLTs, and αMM@PLTs + 3-MA under US irradiation (1.0 MHz, 1.0 W cm−2, 50% duty cycle, 1 min).

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Dec 6:23:539-550. [Abstract]

    Customer Validation
     Effects of 3-MA (200 μM) on INDOM-activated expression levels of Atg5, Beclin-1, P62, and LC3-Ⅰ/Ⅱ detected by Western blot.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2022 Dec 6:23:539-550. [Abstract]

    Customer Validation
     Immunofluorescence assay exhibited the effects of INDOM on the activation of autophagy in LECs. The number of LC3 stained puncta (green) in LECs increased significantly in the group treated with 300 μM INDOM for 24 h; and 200 μM 3-MA inhibited the effect of INDOM (Scale bar, 100 μm).

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Brain Behav Immun. 2019 Jul:79:244-255. [Abstract]

    Customer Validation
     Immunoblot analysis of NLRP3 in LPS-primed macrophages treated with KA (10 μM) or concurrently with autophagy inhibitor (3-MA, 5 mM). Statistical analysis of NLRP3 intensity during 3-MA or zaprinast treatment is shown.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119. [Abstract]

    Customer Validation
     Western blotting image protein levels of GRP78/Bip in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).The cells were treated for 10, 20, and 30 h. (A) The image of GRP78/BiP for 10 h. (B) The image of GRP78/BiP for 20 h. (C) The image of GRP78/BiP for 30 h.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119. [Abstract]

    Customer Validation
     Western blotting image protein levels of LC3-I & LC3-II in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin). (A) The image of LC3-I & LC3-II for 10 h. (B) The image of LC3-I & LC3-II for 20 h. (C) The image of LC3-I & LC3-II for 30 h.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119. [Abstract]

    Customer Validation
     Western blotting image protein levels of p62 in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin). (A) The image of p62 for 10 h. (B) The image of p62 for 20 h. (C) The image of p62 for 30 h.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Front Endocrinol. 2019 Jun 17:10:370. [Abstract]

    Customer Validation
     LC3 II expression is regulated in CQ- or 3-MA-treated GnRH-OE Panc1 cells.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534. [Abstract]

    Customer Validation
     CRC cells are treated with vehicle, BFA (100 nM), 3-MA (10 mM), or in combination for 24 h. Immunoblot analysis is used to detect LC3 turnover.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Cell Biochem. 2019 Mar;120(3):4366-4374. [Abstract]

    Customer Validation
     Representative Western blots show LC3 I/II and p62 protein expression in kidneys of vehicle group and 3-MA group mice at 72 hours after the CDDP treatment.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2018 Jun;41(6):3221-3230. [Abstract]

    Customer Validation
     Western blot analysis is used to measure LC3, p62 and Beclin‑1 expression following the treatment of H9C2 cells with si‑miRNA‑30e and 3‑MA.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2018 May;41(5):2535-2544. [Abstract]

    Customer Validation
     MC3T3-E1 cells are treated with AICAR (10 μM) in the presence or absence of 3-MA (5 mM) or CQ (10 μM) for 24 h, after which western blot analysis is conducted.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Int J Oncol. 2018 Sep;53(3):1363-1373. [Abstract]

    Customer Validation
     The protein expression levels of LC3 and Beclin-1 proteins in HCT-116 cells. Cells are treated with 100 μg/mL active fraction of clove (AFC) for 48 h with or without pretreatment of 2 mM 3-MA or 1 nM Baflomycin A1 (BA) for 1 h.

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    3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2018 Oct;22(10):5132-5144. [Abstract]

    Customer Validation
     Melatonin regulation of mitophagy activity in the diabetic myocardium, Protein expression with representative gel blots of LC3 I/II, GAPDH (loading control).

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Dermatol Sci. 2018 Jan;89(1):11-18. [Abstract]

    Customer Validation
     Inhibitory effects of LED 585 nm on melanogenesis is reversed by 3-MA.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: J Immunol Res. 2018 Dec 24:2018:9807139. [Abstract]

    Customer Validation
     Western blot assays show that 3-MA abrogates the Alda-1 induced expression increases in LC3BⅡ and Bcl2 and decreases in P62 and Bax.

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    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2018 Feb 1;50(2):144-155. [Abstract]

    Customer Validation
     Autophagy is involved in Rg1-inhibited apoptosis in macrophages Macrophages with serum deprivation are treated with 50 μM Rg1 for 48 h in the absence or presence of 3-MA (5 mM). The protein expression level of cleaved caspase-3 determined by western blot analysis in Raw264.7 macrophages.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: PLoS One. 2018 Aug 6;13(8):e0201920. [Abstract]

    Customer Validation
     The pretreatment of Z-VAD-FMK significantly reduces the levels of cleaved caspase-3 and cleaved PARP. The pretreatment of 3-MA blocks the conversion of LC3-I to LC3-II.

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    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Cell Physiol Biochem. 2018;48(6):2318-2336. [Abstract]

    Customer Validation
     Cells are pretreated with 5 mM 3-MA or 2 μM for 1 h and then exposed to 40 μM EPA and 10 μM Rp for 48 h. Cell extracts are prepared and subjected to western blot analysis.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Biomed Res. 2018;39(2):87-94. [Abstract]

    Customer Validation
     Accumulation of LC3B II and p62 proteins in the livers of chloroform-treated mice is reduced by 3-MA treatment.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Nov 22;8(65):109135-109150. [Abstract]

    Customer Validation
     Cell morphology of MCF-7 treated with RA (20 μM)+ω-3 PUFAs (80 μM) with or without 3-MA (5 mM) for 24h.

  • 1/16

    3-Methyladenine purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96. [Abstract]

    Customer Validation
     The hepatic stellate cells (HSCs) activation is down-regulated along with autophagy following DMKG or 3MA treatment in vitro. Western blot analysis of LC3B, Beclin-1, α-SMA, and collagen-I expression from lysates of HSC-T6 incubated with 4mM DMKG or 10 mM 3MA for 12 h.

  • 3-Methyladenine