1. PI3K/Akt/mTOR
    Autophagy
  2. PI3K
    Autophagy

3-Methyladenine (Synonyms: 3-MA)

Cat. No.: HY-19312 Purity: 99.84%
Data Sheet SDS Handling Instructions

3-Methyladenine is a selective PI3K inhibitor with IC50 of 25 μM and 60 μM for Vps34 and PI3Kγ, respectively, and it can inhibit all PtdIns3Ks at 10 mM.

For research use only. We do not sell to patients.
3-Methyladenine Chemical Structure

3-Methyladenine Chemical Structure

CAS No. : 5142-23-4

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $55 In-stock
50 mg $50 In-stock
100 mg $80 In-stock
200 mg $140 In-stock
500 mg $270 In-stock
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Customer Review

    3-Methyladenine purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96.

    The hepatic stellate cells (HSCs) activation is down-regulated along with autophagy following DMKG or 3MA treatment in vitro. Western blot analysis of LC3B, Beclin-1, α-SMA, and collagen-I expression from lysates of HSC-T6 incubated with 4mM DMKG or 10 mM 3MA for 12 h.

    3-Methyladenine purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96.

    DMKG inhibits autophagy of HSCs via acetyl-coenzyme A and EP300. Western blot analysis of LC3B, Beclin-1, a-SMA, and collagen-I expression from lysates of HSC-T6 incubated with control media, DMKG (4 mM), or Lipoic acid (5 mM) in the presence or absence of C646 (10 mM) for 12 h.

    3-Methyladenine purchased from MCE. Usage Cited in: J Dermatol Sci. 2017 Oct 11.

    Inhibitory effects of LED 585 nm on melanogenesis is reversed by 3-MA.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    3-Methyladenine is a selective PI3K inhibitor with IC50 of 25 μM and 60 μM for Vps34 and PI3Kγ, respectively, and it can inhibit all PtdIns3Ks at 10 mM.

    IC50 & Target

    IC50: 25 μM (Vps34), 60 μM (PI3Kγ)

    In Vitro

    3-methyladenine shows some limited Vps34 preference in vitro, with an IC50 of 25 µM for Vps34 as compared with 60 µM for PtdIns3Kγ, and 3-methyladenine (10 mM) can inhibit all PtdIns3Ks[1]. 3-methyladenine (3-MA, 5 mM) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions. 3-methyladenine induces caspase-dependent cell death in HeLa cells, and the death occurs independently of the inhibition of autophagy. 3-MA (1 mM) significantly shortens the duration of nocodazole-induced-prometaphase arrest[2]. A large number of neurons from the 3-MA group are in the early stage of apoptosis, displaying cell shrinkage, nuclear and chromatin condensation and aggregation at the edge of the nucleus[3].

    In Vivo

    3-methyladenine-treatment significantly aggravates neurological symptoms and apoptotic cell death after SAH[3].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 6.7047 mL 33.5233 mL 67.0466 mL
    5 mM 1.3409 mL 6.7047 mL 13.4093 mL
    10 mM 0.6705 mL 3.3523 mL 6.7047 mL
    Please refer to the solubility information to select the appropriate solvent.
    Cell Assay
    [2]

    Cell viability is determined by a trypan blue exclusion assay. Cells are cultured in the medium with 3-Methyladenine. Both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2×106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    3-Methyladenine is dissolved in normal saline.

    Animals are positioned in a stereotactic frame and treated with 5-μL intracerebral ventricular injections of autophagy inducer RAP (20 pmol), inhibitor 3-Methyladenine (3-MA, 400 nmol) or vehicle 20 min before SAH onset. Intracerebral ventricular injections are made into the right lateral ventricle at coordinates of 0.8 mm anterior to posterior bregma, 1.5 mm mid to lateral, 3.8 mm dorsal to ventral. After the injection, the syringe is left in the site for 30 s before removal, and the burr hole is filled wath bone wax. Drug doses are selected on the basis of previous published studies and pilot experiments in our laboratory. 3-MA is dissolved in normal saline by heating the solution to 60-70°C immediately before treatment. RAP is first dissolved in ethanol and then diluted with normal saline (final ethanol concentration < 2%). MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    149.15

    Formula

    C₆H₇N₅

    CAS No.

    5142-23-4

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 1.5 mg/mL; DMSO: 6.8 mg/mL (Need ultrasonic); H2O: 8.82 mg/mL (Need ultrasonic)

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    Purity: 99.84%

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    Inquiry Information

    Product Name:
    3-Methyladenine
    Cat. No.:
    HY-19312
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