2. Cytoskeleton Autophagy Apoptosis
  3. Dynamin Mitophagy Autophagy Apoptosis
  4. Mdivi-1

Mdivi-1  (Synonyms: Mitochondrial division inhibitor 1)

Cat. No.: HY-15886 Purity: 99.73%
COA Handling Instructions

Mdivi-1 is a selective dynamin-related protein 1 (Drp1) inhibitor. Mdivi-1 is a mitochondrial division/mitophagy inhibitor.

For research use only. We do not sell to patients.

Mdivi-1 Chemical Structure

Mdivi-1 Chemical Structure

CAS No. : 338967-87-6

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Free Sample (0.1 - 0.5 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 55 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 55 In-stock
Solid
5 mg USD 50 In-stock
10 mg USD 65 In-stock
50 mg USD 190 In-stock
100 mg USD 290 In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 115 publication(s) in Google Scholar

Mdivi-1 Related Antibodies

Top Publications Citing Use of Products

106 Publications Citing Use of MCE Mdivi-1

WB
IHC

    Mdivi-1 purchased from MCE. Usage Cited in: Vet Microbiol. 2023 May.

    S.aureus-induced reduction of mitochondrial proteins is blocked by Mdivi-1 (20 μM; 2 h) in BMECs.

    Mdivi-1 purchased from MCE. Usage Cited in: Theranostics. 2019 Feb 28;9(6):1698-1713.  [Abstract]

    Western blotting of SDs and markers of podocyte dedifferentiation with the treatment of PA , BBR + PA and Mdivi1+PA.

    Mdivi-1 purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Mar 8;10(3):232.  [Abstract]

    HepG2/ADM cells are pretreated with mdivi-1 (10 μM) for 1 h, followed by treatment with B5G1 (6 μM) for another 24 h. Mitochondrial colocalization with LC3 is detected by immunofluorescence.

    Mdivi-1 purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Mar 8;10(3):232.  [Abstract]

    HepG2/ADM cells are pretreated with mdivi-1 (10 μM) for 1 h, followed by treatment with B5G1 (6 μM) for another 48 h. Apoptosis-related proteins expression level is detected by western blotting. β-actin was used as a loading control.

    Mdivi-1 purchased from MCE. Usage Cited in: Brain Res. 2019 May 15;1711:68-76.  [Abstract]

    Levels of CytC in cytosol (Cyto-CytC), CytC in mitochondria (Mito- CytC) and cleaved caspase-3 in hippocampus are determined by western blot with the treatment of sham, HIR, MDV or FK506.

    Mdivi-1 purchased from MCE. Usage Cited in: J Pineal Res. 2018 Sep;65(2):e12491.  [Abstract]

    Inhibition of mitochondrial fission with mdivi-1 or Drp1 siRNA suppresses hyperglycemia-induced oxidative stress. Protein expression of Nox4.

    Mdivi-1 purchased from MCE. Usage Cited in: Transl Res. 2018 Apr;194:68-78.  [Abstract]

    Representative western blot analysis of pDrp1-616 and total Drp1 protein expression in the hippocampus (left). Tubulin is used as the loading control. Protein expressions are quantified by western blot band intensity (right).

    Mdivi-1 purchased from MCE. Usage Cited in: Free Radic Biol Med. 2017 Nov;112:336-349.  [Abstract]

    Mdivi-1 administration reduces NF-κB-dependent inflammation. A. Western blot assay and quantitative analysis of Cyto-p65 (A) and Nec-p65 (B) expression. ß-actin and H3 are used as internal controls of cytosolic and nuclear subfractions, respectively.

    Mdivi-1 purchased from MCE. Usage Cited in: Exp Neurol. 2017 Sep;295:116-124.  [Abstract]

    Mdivi-1 treatment inhibits the expression of DRP1, alleviates ROS and reduces the expression of NLRP3 and cleaved caspase-1 at 24 h after SAH. Representative Western blots showing levels of DRP1.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Mdivi-1 is a selective dynamin-related protein 1 (Drp1) inhibitor. Mdivi-1 is a mitochondrial division/mitophagy inhibitor.

    In Vitro

    Mdivi-1 inhibits Dnm1 GTPase activity in a dose-dependent manner, with an estimated EC50 of 1-10 μM. Mdivi-1 increases the apparent K0.5 for GTP, lowers the apparent Vmax for GTP hydrolysis, and causes an increase in the Hill coefficient observed for GTP in the Dnm1 GTP hydrolysis reaction[1]. Cells treated with mdivi-1 display decreased cytochrome c release and a reduced rate of phosphatidylserine exposure on their surface following apoptosis induction, consistent with an inhibition of apoptosis and with previous studies using other strategies to compromise DRP1 activity[2]. Mdivi-1 results in apoptotic cell death in ischemic retina[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Mdivi-1 Related Antibodies
    In Vivo

    The mitochondrial division DRP, Dnm1, is the target of mdivi-1 in vivo. Mdivi-1 quantitatively blocks GMPPCP- dependent Dnm1 self-assembly in a concentration range similar to its effects on mitochondrial division in vivo[1]. Mdivi-1 (50 mg/kg, i.p.) significantly decreases GFAP protein expression in the normal mouse retina[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    353.22

    Appearance

    Solid

    Formula

    C15H10Cl2N2O2S
    CAS No.
    SMILES

    O=C1N(C2=C(Cl)C=C(Cl)C(OC)=C2)C(S)=NC3=C1C=CC=C3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 160 mg/mL (452.98 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8311 mL 14.1555 mL 28.3110 mL
    5 mM 0.5662 mL 2.8311 mL 5.6622 mL
    10 mM 0.2831 mL 1.4155 mL 2.8311 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  17% Polyethylene glycol 12-hydroxystearate in saline

      Solubility: 10 mg/mL (28.31 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 4 mg/mL (11.32 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 4 mg/mL (11.32 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 4 mg/mL (11.32 mM); Clear solution

    • 5.

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 2.5 mg/mL (7.08 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.73%

    COA (253 KB) LCMS (138 KB)

    Handling Instructions (2659 KB)
    References
    Kinase Assay
    [1]

    All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Cell Assay
    [1]

    YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References
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    Mdivi-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    Mdivi-1338967-87-6Mitochondrial division inhibitor 1Mdivi1Mdivi 1Mitochondrial division inhibitor1Mitochondrial division inhibitor-1DynaminMitophagyAutophagyApoptosisMitochondrial AutophagyInhibitorinhibitorinhibit

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