1. Cytoskeleton
  2. Dynamin

Mdivi-1 (Synonyms: Mitochondrial division inhibitor 1)

Cat. No.: HY-15886 Purity: 98.75%
Handling Instructions

Mdivi-1 is a selective dynamin-related protein 1 (Drp1) inhibitor.

For research use only. We do not sell to patients.

Mdivi-1 Chemical Structure

Mdivi-1 Chemical Structure

CAS No. : 338967-87-6

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
10 mg USD 65 In-stock
Estimated Time of Arrival: December 31
50 mg USD 190 In-stock
Estimated Time of Arrival: December 31
100 mg USD 290 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

    Mdivi-1 purchased from MCE. Usage Cited in: Free Radic Biol Med. 2017 Nov;112:336-349.

    Mdivi-1 administration reduces NF-κB-dependent inflammation. A. Western blot assay and quantitative analysis of Cyto-p65 (A) and Nec-p65 (B) expression. ß-actin and H3 are used as internal controls of cytosolic and nuclear subfractions, respectively.

    Mdivi-1 purchased from MCE. Usage Cited in: Exp Neurol. 2017 Sep;295:116-124.

    Mdivi-1 treatment inhibits the expression of DRP1, alleviates ROS and reduces the expression of NLRP3 and cleaved caspase-1 at 24 h after SAH. Representative Western blots showing levels of DRP1.

    Mdivi-1 purchased from MCE. Usage Cited in: Transl Res. 2018 Apr;194:68-78.

    Representative western blot analysis of pDrp1-616 and total Drp1 protein expression in the hippocampus (left). Tubulin is used as the loading control. Protein expressions are quantified by western blot band intensity (right).

    Mdivi-1 purchased from MCE. Usage Cited in: J Pineal Res. 2018 Sep;65(2):e12491.

    Inhibition of mitochondrial fission with mdivi-1 or Drp1 siRNA suppresses hyperglycemia-induced oxidative stress. Protein expression of Nox4.

    Mdivi-1 purchased from MCE. Usage Cited in: Brain Res. 2019 Jan 16. pii: S0006-8993(19)30035-6.

    Levels of CytC in cytosol (Cyto-CytC), CytC in mitochondria (Mito- CytC) and cleaved caspase-3 in hippocampus are determined by western blot with the treatment of sham, HIR, MDV or FK506.

    Mdivi-1 purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Mar 8;10(3):232.

    HepG2/ADM cells are pretreated with mdivi-1 (10 μM) for 1 h, followed by treatment with B5G1 (6 μM) for another 24 h. Mitochondrial colocalization with LC3 is detected by immunofluorescence.

    Mdivi-1 purchased from MCE. Usage Cited in: Cell Death Dis. 2019 Mar 8;10(3):232.

    HepG2/ADM cells are pretreated with mdivi-1 (10 μM) for 1 h, followed by treatment with B5G1 (6 μM) for another 48 h. Apoptosis-related proteins expression level is detected by western blotting. β-actin was used as a loading control.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Mdivi-1 is a selective dynamin-related protein 1 (Drp1) inhibitor.

    In Vitro

    Mdivi-1 inhibits Dnm1 GTPase activity in a dose-dependent manner, with an estimated EC50 of 1-10 μM. Mdivi-1 increases the apparent K0.5 for GTP, lowers the apparent Vmax for GTP hydrolysis, and causes an increase in the Hill coefficient observed for GTP in the Dnm1 GTP hydrolysis reaction[1]. Cells treated with mdivi-1 display decreased cytochrome c release and a reduced rate of phosphatidylserine exposure on their surface following apoptosis induction, consistent with an inhibition of apoptosis and with previous studies using other strategies to compromise DRP1 activity[2]. Mdivi-1 results in apoptotic cell death in ischemic retina[3].

    In Vivo

    The mitochondrial division DRP, Dnm1, is the target of mdivi-1 in vivo. Mdivi-1 quantitatively blocks GMPPCP- dependent Dnm1 self-assembly in a concentration range similar to its effects on mitochondrial division in vivo[1]. Mdivi-1 (50 mg/kg, i.p.) significantly decreases GFAP protein expression in the normal mouse retina[3].

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 32 mg/mL (90.60 mM)

    H2O : < 0.1 mg/mL (insoluble)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8311 mL 14.1555 mL 28.3110 mL
    5 mM 0.5662 mL 2.8311 mL 5.6622 mL
    10 mM 0.2831 mL 1.4155 mL 2.8311 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 2.5 mg/mL (7.08 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (7.08 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (7.08 mM); Clear solution

    References
    Kinase Assay
    [1]

    All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay
    [1]

    YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    353.22

    Formula

    C₁₅H₁₀Cl₂N₂O₂S

    CAS No.

    338967-87-6

    SMILES

    O=C1N(C2=C(Cl)C=C(Cl)C(OC)=C2)C(S)=NC3=C1C=CC=C3

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 98.75%

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name

     

    Salutation

    Applicant name *

     

    Email address *

    Phone number *

     

    Organization name *

    Country or Region *

     

    Requested quantity *

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Mdivi-1
    Cat. No.:
    HY-15886
    Quantity:

    Mdivi-1

    Cat. No.: HY-15886