1. Cytoskeleton
  2. Dynamin

Mdivi-1 (Synonyms: Mitochondrial division inhibitor 1)

Cat. No.: HY-15886 Purity: 98.75%
Handling Instructions

Mdivi-1 a selective cell-permeable inhibitor of mitochondrial division dynamin-related GTPase (DRP1) and mitochondrial division Dynamin I (Dnm1).

For research use only. We do not sell to patients.

Mdivi-1 Chemical Structure

Mdivi-1 Chemical Structure

CAS No. : 338967-87-6

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 55 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
5 mg USD 50 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
10 mg USD 65 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
50 mg USD 190 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
100 mg USD 290 In-stock
Stock in Sweden
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

    Mdivi-1 purchased from MCE. Usage Cited in: Free Radic Biol Med. 2017 Aug 5;112:336-349.

    Mdivi-1 administration reduces NF-κB-dependent inflammation. A. Western blot assay and quantitative analysis of Cyto-p65 (A) and Nec-p65 (B) expression. ß-actin and H3 are used as internal controls of cytosolic and nuclear subfractions, respectively.

    Mdivi-1 purchased from MCE. Usage Cited in: Exp Neurol. 2017 Jun 2;295:116-124.

    Mdivi-1 treatment inhibits the expression of DRP1, alleviates ROS and reduces the expression of NLRP3 and cleaved caspase-1 at 24 h after SAH. Representative Western blots showing levels of DRP1.

    Mdivi-1 purchased from MCE. Usage Cited in: Exp Neurol. 2017 Jun 2;295:116-124.

    Nec-1 treatment inhibits RIP1-RIP3, p-DRP1 activation and reduces the expression of NLRP3 and cleaved caspase-1 at 24 h after SAH. Representative Western blots showing levels of RIP1, RIP3, p-DRP1, DRP1, NLRP3 and cleaved caspase-1.

    Mdivi-1 purchased from MCE. Usage Cited in: Transl Res. 2018 Apr;194:68-78.

    Representative western blot analysis of pDrp1-616 and total Drp1 protein expression in the hippocampus (left). Tubulin is used as the loading control. Protein expressions are quantified by western blot band intensity (right).

    Mdivi-1 purchased from MCE. Usage Cited in: J Pineal Res. 2018 Mar 25:e12491.

    Inhibition of mitochondrial fission with mdivi-1 or Drp1 siRNA suppresses hyperglycemia-induced oxidative stress. Protein expression of Nox4.

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    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Mdivi-1 a selective cell-permeable inhibitor of mitochondrial division dynamin-related GTPase (DRP1) and mitochondrial division Dynamin I (Dnm1).

    In Vitro

    Mdivi-1 inhibits Dnm1 GTPase activity in a dose-dependent manner, with an estimated EC50 of 1-10 μM. Mdivi-1 increases the apparent K0.5 for GTP, lowers the apparent Vmax for GTP hydrolysis, and causes an increase in the Hill coefficient observed for GTP in the Dnm1 GTP hydrolysis reaction[1]. Cells treated with mdivi-1 display decreased cytochrome c release and a reduced rate of phosphatidylserine exposure on their surface following apoptosis induction, consistent with an inhibition of apoptosis and with previous studies using other strategies to compromise DRP1 activity[2]. Mdivi-1 results in apoptotic cell death in ischemic retina[3].

    In Vivo

    The mitochondrial division DRP, Dnm1, is the target of mdivi-1 in vivo. Mdivi-1 quantitatively blocks GMPPCP- dependent Dnm1 self-assembly in a concentration range similar to its effects on mitochondrial division in vivo[1]. Mdivi-1 (50 mg/kg, i.p.) significantly decreases GFAP protein expression in the normal mouse retina[3].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 2.8311 mL 14.1555 mL 28.3110 mL
    5 mM 0.5662 mL 2.8311 mL 5.6622 mL
    10 mM 0.2831 mL 1.4155 mL 2.8311 mL
    Please refer to the solubility information to select the appropriate solvent.
    Kinase Assay
    [1]

    All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay
    [1]

    Mdivi-1 is dissolved in DMSO.

    YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    353.22

    Formula

    C₁₅H₁₀Cl₂N₂O₂S

    CAS No.

    338967-87-6

    SMILES

    O=C1N(C2=C(Cl)C=C(Cl)C(OC)=C2)C(S)=NC3=C1C=CC=C3

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 32 mg/mL

    Mdivi-1 is dissolved in DMSO (50 mg/mL) as stock. It is diluted in sterile saline before injection[4].

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    References

    Purity: 98.75%

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    Product Name:
    Mdivi-1
    Cat. No.:
    HY-15886
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