Autophagy is involved in degradation of AQP1 in response to an acute decrement in tonicity

  • iScience. 2023 Nov 20;26(12):108485. doi: 10.1016/j.isci.2023.108485.
Xiangdong Guo  1  2 ,  Long Xu  1  2 ,  Yonglun Kong  2  3 ,  Meng Li  1  2 ,  Qingxian Zhai  2  3 ,  Baien Liang  2  3 ,  Xiaoduo Zhao  2  3 ,  Luosha Long  1  2 ,  Meiying Huang  2  3 ,  Weidong Wang  2  3 ,  Chunling Li  1  2
Affiliations
  • 1. Department of Physiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
  • 2. Institute of Hypertension, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
  • 3. Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
PMID: 38094243 DOI: 10.1016/j.isci.2023.108485
Abstract

Renal medullary aquaporin-1 (AQP1) plays an important role in the urinary concentration. This study aimed to investigate the regulation of AQP1 by low osmotic stress and a potential role of Autophagy. Low osmotic stress induced a dramatically decreased AQP1 protein expression in murine inner medullary collecting duct 3 (mIMCD3) cells, which was associated with a marked activation of Autophagy. Inhibition of Autophagy by 3-methyladenine (3-MA), chloroquine, or knockdown of autophagy-related protein 5 (ATG5) prevented the decrease in AQP1 protein abundance. Rapamycin-induced Autophagy was associated with a decreased AQP1 protein expression and an enhanced interaction between AQP1 and ATG5 in mIMCD3 cells under low osmotic stress. In kidney inner medulla of mice given a 3% NaCl solution, activation of Autophagy was associated with decreased AQP1 protein expression, which was prevented by 3-MA. In conclusion, low osmotic stress induced Autophagy which contributed to the decreased AQP1 protein expression in the renal medulla.

Keywords
Biological sciences; Cell biology; Molecular biology.
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