Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32

  • Biochem J. 1996 Apr 1;315 ( Pt 1)(Pt 1):21-4. doi: 10.1042/bj3150021.
E A Slee  1 ,  H Zhu ,  S C Chow ,  M MacFarlane ,  D W Nicholson ,  G M Cohen
Affiliations
  • 1. MRC Toxicology Unit, University of Leicester, Lancaster Road, Leicester, U.K.
PMID: 8670109 DOI: 10.1042/bj3150021
Abstract

Interleukin-1 beta converting enzyme (ICE)-like proteases, which are synthesized as inactive precursors, play a key role in the induction of Apoptosis. We now demonstrate that benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK), an ICE-like protease inhibitor, inhibits Apoptosis by preventing the processing of CPP32 to its active form. These results suggest that novel inhibitors of Apoptosis can be developed which prevent processing of proforms of ICE-like proteases.

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