1. Apoptosis
  2. Ferroptosis
  3. iFSP1

iFSP1 

Cat. No.: HY-136057 Purity: 99.44%
Handling Instructions

iFSP1 is a potent, selective and glutathione-independent inhibitor of ferroptosis suppressor protein 1 (FSP1) (AIFM2) with an EC50 of 103 nM. iFSP1 selectively induces ferroptosis in GPX4-knockout cells which overexpressed FSP1. iFSP1 is able to sensitize a variety of human cancer cell lines to the ferroptosis inducer, such as (1S,3R)-RSL3 (HY-100218A).

For research use only. We do not sell to patients.

iFSP1 Chemical Structure

iFSP1 Chemical Structure

CAS No. : 150651-39-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 53 In-stock
Estimated Time of Arrival: December 31
10 mg USD 48 In-stock
Estimated Time of Arrival: December 31
50 mg USD 213 In-stock
Estimated Time of Arrival: December 31
100 mg USD 400 In-stock
Estimated Time of Arrival: December 31
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Description

iFSP1 is a potent, selective and glutathione-independent inhibitor of ferroptosis suppressor protein 1 (FSP1) (AIFM2) with an EC50 of 103 nM. iFSP1 selectively induces ferroptosis in GPX4-knockout cells which overexpressed FSP1. iFSP1 is able to sensitize a variety of human cancer cell lines to the ferroptosis inducer, such as (1S,3R)-RSL3 (HY-100218A)[1].

IC50 & Target

EC50: 103 nM (FSP1)[1]

In Vitro

iFSP1 (0.001-1 μM; 24 hours) inhibits the Gpx4-knockout cell growth as a dose-dependent manner, but does not inhibit the wild type cell growth. Treatment with the ferroptosis inhibitor Lip-1 protects GPX4- knockout cells from iFSP1-induced ferroptosis[1].
iFSP1 (0.001-1 μM; 24 hours) is less efficient than genetic deletion of FSP1, whereas iFSP1 treatment in the FSP1-knockout background had no additive effect to RSL3-induced ferroptosis[1].
iFSP1 (3 μM; 24 hours) treatment results in an obvious toxicity of RSL3 in a panel of genetically engineered (FSP1-knockout) human cancer cell lines[1].
AIFM2: the flavoprotein apoptosis-inducing factor mitochondria-associated 2 is a previously unrecognized anti-ferroptotic gene. AIFM2, which is renamed ferroptosis suppressor protein 1 (FSP1)[1]

Cell Proliferation Assay[1]

Cell Line: Wild-type and Gpx4-knockout Pfa1 or HT 1080 cells overexpressing FSP1–HA
Concentration: 0.001-1 μM
Incubation Time: 24 hours
Result: Was toxic to cells that depend solely (no GPX4 expression detectable) on FSP1 function.

Cell Viability Assay[1]

Cell Line: NCl-H1437, NCl-H1437 FSP1 KO, U-373, U-373 FSP1 KO, MDA-MB-436, MDA-MB-436 FSP1 KO, SW620, SW620 FSP1 KO, MDA-MB-435S, MDA-MB-435S FSP1 KO, A549 and A549 FSP1 KO
Concentration: 3 μM
Incubation Time: 24 hours
Result: Sensitized a variety of human cancer cell lines to the ferroptosis inducer (1S,3R)-RSL3.
Molecular Weight

323.35

Formula

C₂₀H₁₃N₅

CAS No.

150651-39-1

SMILES

N#CC1=C(N)N2C3=CC=CC=C3N=C2C(C#N)=C1C4=CC=C(C)C=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (77.32 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0926 mL 15.4631 mL 30.9262 mL
5 mM 0.6185 mL 3.0926 mL 6.1852 mL
10 mM 0.3093 mL 1.5463 mL 3.0926 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (6.43 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

iFSP1iFSP 1iFSP-1FerroptosisGpx4-knockoutferroptosisRSL3FSP1-knockoutcellcancerFSP1KONCl-H1437U-373MDA-MB-436SW620MDA-MB-435SA549Inhibitorinhibitorinhibit

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iFSP1
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