1. NF-κB
    Autophagy
    Apoptosis
    Anti-infection
  2. NF-κB
    Autophagy
    Apoptosis
    Ferroptosis
    Bacterial
    Antibiotic
  3. Sulfasalazine

Sulfasalazine (Synonyms: NSC 667219)

Cat. No.: HY-14655 Purity: 99.42%
Handling Instructions

Sulfasalazine (NSC 667219) is an anti-rheumatic agent for the treatment of rheumatoid arthritis and ulcerative colitis. Sulfasalazine is reported to suppress NF-κB activity.

For research use only. We do not sell to patients.

Sulfasalazine Chemical Structure

Sulfasalazine Chemical Structure

CAS No. : 599-79-1

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Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Sulfasalazine purchased from MCE. Usage Cited in: Cell Res. 2018 Dec;28(12):1171-1185.

    Melanoma A375 cells are treated with SSZ (Sulfasalazine, 125 μM) with or without FeSO4 (100 μM) for 6 h to detect the ROS level or 24 h to assess the pyroptotic features (including morphology, GSDME cleavage, and LDH release), unless specifically defined.

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    Description

    Sulfasalazine (NSC 667219) is an anti-rheumatic agent for the treatment of rheumatoid arthritis and ulcerative colitis. Sulfasalazine is reported to suppress NF-κB activity.

    IC50 & Target[1]

    RelA

     

    Autophagy

     

    In Vitro

    Treatment of SW620 colon cells with sulfasalazine inhibits TNFα-, LPS-, or phorbol ester-induced NFκB activation. NFκB-dependent transcription is inhibited by sulfasalazine at micro- to millimolar concentrations. TNFα-induced nuclear translocation of NFκB is prevented by sulfasalazine through inhibition of IκBα degradation[1]. Pre-incubation with 5 mM of sulfasalazine alone significantly increases basal mRNA expression of all pro-inflammatory cytokines with levels of IL-6 mRNA increased by 80-fold compared with vehicle control[2]. Once digested, sulfasalazine is cleaved into sulfapyridine and 5-aminosalicylic acid by colonic bacteria, and the latter, too, is reported to suppress NF-kappaB activity[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    At low doses (0.25 mM), SAS is able to suppress glioma growth by over 60% compared to untreated controls[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Clinical Trial
    Molecular Weight

    398.39

    Formula

    C₁₈H₁₄N₄O₅S

    CAS No.

    599-79-1

    SMILES

    OC(C1=C(O)C=CC(/N=N/C2=CC=C(S(=O)(NC3=NC=CC=C3)=O)C=C2)=C1)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    NH4OH : 150 mg/mL (376.52 mM; ultrasonic and adjust pH to 9 with NH4OH)

    DMSO : 80 mg/mL (200.81 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5101 mL 12.5505 mL 25.1010 mL
    5 mM 0.5020 mL 2.5101 mL 5.0202 mL
    10 mM 0.2510 mL 1.2551 mL 2.5101 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Kinase Assay

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay
    [1]

    Sulfasalazine is dissolved in culture medium. SW620 cells are grown in Dulbecco’s modified Eagle medium, supplemented with 10% heat-inactivated FCS, 2 mmol/liter glutamine, and 1% (wt/vol) penicillin/streptomycin. SW620 cells are transfected with the 3xIgkBLuc reporter construct. After 18 h, cells are incubated with either medium alone or with sulfasalazine (0.1, 0.2, 0.5, 1, 2, 5 mM) before stimulation with TNFα, LPS, or PMA. Luciferase assay is performed[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Mice: Sulfasalazine is dissolved in 0.1 M NaOH, and then neutralized by titrating with 0.1 M HCl. U-87MG glioma cells are implanted into the cranium of a SCID mouse. After 7 days, animals are randomized into three groups of five animals each. One group receives 1 mL i.p. saline injections twice daily for 3 weeks. The two test groups receives 8 mg of sulfasalazine in 1 mL saline twice daily for 3 weeks. Tumor growth and animal health were monitored. After perfusion with 4% paraformaldehyde, mouse brains were collected, rinsed, and placed in 30% sucrose[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    SulfasalazineNSC 667219NSC667219NSC-667219NF-κBAutophagyApoptosisFerroptosisBacterialAntibioticNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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    Product Name:
    Sulfasalazine
    Cat. No.:
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