1. Apoptosis
  2. Ferroptosis
  3. Liproxstatin-1

Liproxstatin-1 

Cat. No.: HY-12726 Purity: 98.15%
Handling Instructions

Liproxstatin-1 is a potent ferroptosis inhibitor, with IC50 of approximately 38 nM.

For research use only. We do not sell to patients.

Liproxstatin-1 Chemical Structure

Liproxstatin-1 Chemical Structure

CAS No. : 950455-15-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 106 In-stock
Estimated Time of Arrival: December 31
5 mg USD 96 In-stock
Estimated Time of Arrival: December 31
10 mg USD 168 In-stock
Estimated Time of Arrival: December 31
50 mg USD 708 In-stock
Estimated Time of Arrival: December 31
100 mg USD 948 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 11 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Liproxstatin-1 purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2019 Sep 4. pii: S0006-291X(19)31701-2.

    The ROS and inflammatory cytokines levels increase in acute RILI. The serum levels of inflammatory cytokines increase in RILI. Elisa was used to measure the levels of inflammatory cytokines (TNF-a, IL-6, IL-10 and TGF-b1) at day 15, day 30 and day 60.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Liproxstatin-1 is a potent ferroptosis inhibitor, with IC50 of approximately 38 nM.

    IC50 & Target

    EC50: 38 nM (ferroptosis)[2]

    In Vitro

    Liproxstatin-1 prevents BODIPY 581/591 C11 oxidation in Gpx4−/− cells. Moreover, Liproxstatin-1 does not interfere with other classical types of cell death, such as TNFα-induced apoptosis and H2O2-induced necrosis, and in the bona fide L929 model of TNFα/zvad-induced necroptosis[1]. Liproxstatin-1 has great antiferroptotic activity with EC50 of appr 38 nM. Fer-1 and Liproxstatin-1 are inherently good, but not great, radical-trapping antioxidants, but they are excellent in phospholipid bilayers. Fer-1 (10 μM) and Liproxstatin-1 (10 μM) do not exhibit significant inhibitory activity in the 15-LOX-1 overexpressing cells, and the concentration is almost 1000-fold higher than their EC50s for subverting RSL3-induced ferroptosis in these cells (15 and 27 nM, respectively)[2].

    In Vivo

    Liproxstatin-1 (10 mg/kg, i.p.) suppresses ferroptosis in human cells, Gpx4−/− kidney and in an ischaemia/reperfusion-induced tissue injury model[1].

    Molecular Weight

    340.85

    Formula

    C₁₉H₂₁ClN₄

    CAS No.

    950455-15-9

    SMILES

    ClC1=CC(CNC2=NC3=CC=CC=C3NC24CCNCC4)=CC=C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 31 mg/mL (90.95 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9338 mL 14.6692 mL 29.3384 mL
    5 mM 0.5868 mL 2.9338 mL 5.8677 mL
    10 mM 0.2934 mL 1.4669 mL 2.9338 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (7.33 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (7.33 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (7.33 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    To induce the knockout of Gpx4, cells are seeded onto 96-well plates (1,000 cells per well) and treated with 1 μM 4-OH-tamoxifen (TAM) after plating. Cell viability is assessed at different time points after treatment (usually 72 h) using AquaBluer, unless stated otherwise, as an indicator of viable cells. Alternatively, cell death is also quantified by measuring released lactate dehydrogenase (LDH) activity using the Cytotoxicity Detection Kit.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    Animals includes in the treatment study of inducible Gpx4−/− mice are equally distributed between sex and weight, with typically 8-10 weeks of age. The average weight within the groups is between 22 and 24 g. Groups are formed to have comparable numbers of females/males of the same age. Animal weight is arranged to have a similar distribution between females and males. For the pharmacological inhibitor experiments, CreERT2;Gpx4fl/fl mice are injected on day 1 and 3 with 0.5 mg TAM dissolved in Miglyol. On day 4, compound treatment is started (Liproxstatin-1: 10 mg/kg) along with vehicle control (1% dimethylsulphoxide (DMSO) in PBS). Liproxstatin-1 and vehicle control are administered once daily by i.p. injection. Survival analysis is performed using the GraphPad Prism software and statistical analysis is done according to the log-rank test.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 98.15%

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    Keywords:

    Liproxstatin-1Liproxstatin1Liproxstatin 1FerroptosisInhibitorinhibitorinhibit

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    Product name:
    Liproxstatin-1
    Cat. No.:
    HY-12726
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