ML-210

Name: ML-210
Brand: MedChemExpress
SKU: HY-100003
Rating: 5.0 (29 reviews)

Cat. No.: HY-100003 Purity: 99.93%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

ML-210 is a selective and covalent glutathione peroxidase 4 (GPX4) inhibitor with an EC₅₀ of 30 nM. ML-210 binds the GPX4 selenocysteine residue. ML-210 has anti-cancer activity.

For research use only. We do not sell to patients.

CAS No. : 1360705-96-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 29 publication(s) in Google Scholar

29 Publications Citing Use of MCE ML-210

Cell Proliferation/Viability Assay

: ML-210 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2025 Aug 15;11(33):eadx6587. [Abstract]; Viability of WT and TMEM16F KO RMA cells treated with ML210 for 12 hours.

: ML-210 purchased from MedChemExpress. Usage Cited in: Biomark Res. 2025 Jan 23;13(1):17. [Abstract]; Cell viability of NCI-H1299 ACSL4^WT or ACSL4^KO cells treated with 2μM ML-210 at the indicated concentrations after 10 h.

: ML-210 purchased from MedChemExpress. Usage Cited in: Nature. 2024 Feb;626(7998):411-418. [Abstract]; Cell viability of WT and CYP51A1, MSMO1, EBP, SC5D KO HEK293T cells treated with ML210 for 8-10 h.

: ML-210 purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2024 Jun;20(6):699-709. [Abstract]; The effect of BAPTA-AM (5 μM) on ML210-induced ferroptosis in MDA-MB-468 cells for 6 h.

: ML-210 purchased from MedChemExpress. Usage Cited in: Cell Discov. 2022 May 3;8(1):40. [Abstract]; U2OS cells were treated with ML210 (10 µM; 0.5-8 h) for different time as indicated. O-GlcNAcylation levels were detected by immunoblotting with antibodies against O-GlcNAc and β-actin.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Glutathione Peroxidase Isoform Specific Products:

View All Isoforms

GPX1 GPX4

Biological Activity
Purity & Documentation
References
Customer Review

Description

ML-210 is a selective and covalent glutathione peroxidase 4 (GPX4) inhibitor with an EC₅₀ of 30 nM. ML-210 binds the GPX4 selenocysteine residue. ML-210 has anti-cancer activity^[1]^[2].

IC₅₀ & Target

GPX4

Cellular Effect

Cell Line	Type	Value	Description	References
A-375	IC₅₀	0.3 μM Compound: ML210; 3	Antiproliferative activity against human A-375 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human A-375 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37087895]
ASPC1	IC₅₀	342 nM Compound: ML210	Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay Induction of ferroptosis in human ASPC1 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
BJ	IC₅₀	107 nM Compound: 2y	Cytotoxicity against human BJ cells expressing HRAS G12V mutant with alternative oncogenic constructs after 48 hrs by alamar blue assay Cytotoxicity against human BJ cells expressing HRAS G12V mutant with alternative oncogenic constructs after 48 hrs by alamar blue assay	[PMID: 22297109]
Calu-1	IC₅₀	0.9 μM Compound: ML210; 3	Antiproliferative activity against human Calu-1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human Calu-1 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37087895]
Calu-1	IC₅₀	630 nM Compound: ML210	Antiproliferative activity against human Calu-1 cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay Antiproliferative activity against human Calu-1 cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay	[PMID: 36603510]
HCT-116	IC₅₀	> 1 μM Compound: ML-210	Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis	[PMID: 35984756]
HCT-116	IC₅₀	> 10 nM Compound: ML210	Induction of ferroptosis in human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay Induction of ferroptosis in human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
HEK-293T	IC₅₀	0.2 μM Compound: ML210; 3	Cytotoxicity against HEK293T cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against HEK293T cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 37087895]
HEK-293T	IC₅₀	430 nM Compound: ML210	Antiproliferative activity against human HEK293T cells incubated for 24 hrs Antiproliferative activity against human HEK293T cells incubated for 24 hrs	[PMID: 37098297]
HT-1080	IC₅₀	0.019 μM Compound: ML210	Antiproliferative activity against human HT-1080 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human HT-1080 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 38838547]
HT-1080	IC₅₀	0.022 μM Compound: ML-210	Cytotoxicity against human HT-1080 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis Cytotoxicity against human HT-1080 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis	[PMID: 35984756]
HT-1080	IC₅₀	0.1 μM Compound: ML210; 3	Antiproliferative activity against human HT-1080 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human HT-1080 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37087895]
HT-1080	IC₅₀	0.153 μM Compound: ML210	Antiproliferative activity against human HT-1080 cells assessed as inhibition of cell growth incubated for 24 hrs by CCK-8 assay Antiproliferative activity against human HT-1080 cells assessed as inhibition of cell growth incubated for 24 hrs by CCK-8 assay	[PMID: 38505849]
HT-1080	IC₅₀	410 nM Compound: ML210	Antiproliferative activity against human HT-1080 cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay Antiproliferative activity against human HT-1080 cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay	[PMID: 36603510]
HT-1080	IC₅₀	410 nM Compound: ML210	Antiproliferative activity against human HT-1080 cells incubated for 24 hrs Antiproliferative activity against human HT-1080 cells incubated for 24 hrs	[PMID: 37098297]
HeLa	IC₅₀	0.253 μM Compound: ML-210	Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis	[PMID: 35984756]
MCF7	IC₅₀	0.076 μM Compound: ML210	Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs by CCK8 assay	[PMID: 33725632]
MCF7	IC₅₀	> 20 μM Compound: ML210	Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs in presence of Fer-1 by CCK8 assay Antiproliferative activity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs in presence of Fer-1 by CCK8 assay	[PMID: 33725632]
MDA-MB-231	IC₅₀	0.02 μM Compound: ML-210	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis	[PMID: 35984756]
MDA-MB-231	IC₅₀	0.021 μM Compound: ML210	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 72 hrs by MTT assay	[PMID: 38838547]
MDA-MB-468	IC₅₀	0.066 μM Compound: ML-210	Cytotoxicity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis Cytotoxicity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis	[PMID: 35984756]
MEF	IC₅₀	0.016 μM Compound: ML-210	Cytotoxicity against mouse MEF cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis Cytotoxicity against mouse MEF cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis	[PMID: 35984756]
NCI-H520	IC₅₀	0.012 μM Compound: ML210	Antiproliferative activity against human NCI-H520 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay Antiproliferative activity against human NCI-H520 cells assessed as inhibition of cell growth incubated for 72 hrs by celltiter-glo assay	[PMID: 38838547]
NCI-H522	IC₅₀	0.044 μM Compound: ML-210	Cytotoxicity against human NCI-H522 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis Cytotoxicity against human NCI-H522 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis	[PMID: 35984756]
NCI-H522	IC₅₀	33 nM Compound: ML210	Induction of ferroptosis in human NCI-H522 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay Induction of ferroptosis in human NCI-H522 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 38265413]
NCI-H522	IC₅₀	34.515 μM Compound: ML-210	Cytotoxicity against human NCI-H522 cells assessed as reduction in cell viability incubated for 3 days in presence of ferroptosis inhibitor, liproxstatin-1 by methylene blue staining based analysis Cytotoxicity against human NCI-H522 cells assessed as reduction in cell viability incubated for 3 days in presence of ferroptosis inhibitor, liproxstatin-1 by methylene blue staining based analysis	[PMID: 35984756]
U2OS	IC₅₀	0.822 μM Compound: ML-210	Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis Cytotoxicity against human U2OS cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis	[PMID: 35984756]
WI-38	IC₅₀	0.06 μM Compound: ML-210	Cytotoxicity against human WI-38 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis Cytotoxicity against human WI-38 cells assessed as reduction in cell viability incubated for 3 days by methylene blue staining based analysis	[PMID: 35984756]

In Vitro

ML-210 exhibits cell-killing activity across a panel of 821 cancer cell lines (WM88, LOX-IMVI, CJM, U257, CAKI2, A498, HT1080, MC38, PANC02). ML-210 is a prodrug that requires cellular activation to bind GPX4^[1].
ML-210 has IC₅₀s of 71 nM, 272 nM and 107nM for BJeLR (HRAS_V12), BJeH-LT (without HRAS_V12) and DRD cell lines, respectively^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

475.32

Formula

C₂₂H₂₀Cl₂N₄O₄

CAS No.

1360705-96-9

Appearance

Solid

Color

White to light yellow

SMILES

O=C(N1CCN(C(C2=CC=C(Cl)C=C2)C3=CC=C(Cl)C=C3)CC1)C4=NOC(C)=C4[N+]([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (52.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1038 mL	10.5192 mL	21.0385 mL
5 mM	0.4208 mL	2.1038 mL	4.2077 mL
10 mM	0.2104 mL	1.0519 mL	2.1038 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.26 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (251 KB) HNMR (143 KB) RP-HPLC (168 KB) LCMS (233 KB)

Handling Instructions (2659 KB)

References

[1]. John K. Eaton, et al. Targeting a Therapy-Resistant Cancer Cell State Using Masked Electrophiles as GPX4 Inhibitors. Biorxiv. 2018.

[2]. Weïwer M, et al. Development of small-molecule probes that selectively kill cells induced to express mutant RAS. Bioorg Med Chem Lett. 2012 Feb 15;22(4):1822-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1038 mL	10.5192 mL	21.0385 mL	52.5961 mL
	5 mM	0.4208 mL	2.1038 mL	4.2077 mL	10.5192 mL
	10 mM	0.2104 mL	1.0519 mL	2.1038 mL	5.2596 mL
	15 mM	0.1403 mL	0.7013 mL	1.4026 mL	3.5064 mL
	20 mM	0.1052 mL	0.5260 mL	1.0519 mL	2.6298 mL
	25 mM	0.0842 mL	0.4208 mL	0.8415 mL	2.1038 mL
	30 mM	0.0701 mL	0.3506 mL	0.7013 mL	1.7532 mL
	40 mM	0.0526 mL	0.2630 mL	0.5260 mL	1.3149 mL
	50 mM	0.0421 mL	0.2104 mL	0.4208 mL	1.0519 mL

ML-210 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ML-2101360705-96-9ML210ML 210Glutathione PeroxidaseFerroptosisCovalentglutathioneperoxidaseGPX4selenocysteineresidueanti-cancerInhibitorinhibitorinhibit

Please select a local distributor ナミキ商事株式会社コスモ・バイオ株式会社重松貿易株式会社
Product Name			Requested Quantity *
Applicant Name *			Salutation
Email Address *			Phone Number *
Department			Organization Name *
City			State
Country or Region *
Remarks

Product Name			Lot #
Applicant Name *			Email Address *
Phone Number			Department *
Organization Name *			Country or Region *
Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

ML-210

Customer Review

ML-210 Related Antibodies

29 Publications Citing Use of MCE ML-210

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Glutathione Peroxidase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

ML-210 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

ML-210 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report