FIN56

Name: FIN56
Brand: MedChemExpress
SKU: HY-103087
Rating: 5.0 (24 reviews)

Cat. No.: HY-103087 Purity: 98.03%: Data Sheet
COA

SDS
Handling Instructions
FAQs
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FIN56 is a specific inducer of ferroptosis. FIN56 induces ferroptosis by inducing degradation of GPX4. FIN56 also binds to and activates squalene synthase.

For research use only. We do not sell to patients.

CAS No. : 1083162-61-1

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 24 publication(s) in Google Scholar

Other Forms of FIN56:

FIN56 (Standard) Get quote

24 Publications Citing Use of MCE FIN56

RT-PCR

Cell Proliferation/Viability Assay

Cell Imaging/Staining

Bio/Physico-chemical Assay

: FIN56 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Oct 26;15(1):9261. [Abstract]; BD-PMN was pretreated with FIN56 (1 μM and 5 μM) to activate FDFT1, for 16 hours. The expression levels of TNF, IL-6, IL-18, and IL-1β were detected by qRT-PCR (N = 4, for each group).

: FIN56 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Mar 29;10(13):eadk1200. [Abstract]; Cell viability assay of HT-1080 cells pretreated with or without AS for 1 h and then stimulated with 40 μM FIN56.

: FIN56 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Mar 29;10(13):eadk1200. [Abstract]; LDH release assay of HT-1080 cells pretreated with or without AS for 1 h and then stimulated with 40 μM FIN56.

: FIN56 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Jun 1:S0891-5849(24)00506-9. [Abstract]; FIN56 (20 μM). CBD represses γH2AX production and cell death caused by radiation and ferroptosis inducers. Representative live and dead cell stained by Calcein-AM/PI. Scale bar: 200 μm.

: FIN56 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2022 Jul:187:158-170.; FIN56. Effect of NAC and/or on Gpx4 expression and ferroptosis in MDCK cells under HG treatment. Immunofluorescence staining for Gpx4 (scale bar is 75 μm).

: FIN56 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2022 Jul:187:158-170.; FIN56. Effect of NAC and/or on Gpx4 expression and ferroptosis in MDCK cells under HG treatment. Representative blots of CD71 and Slc7a11.

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Description

FIN56 is a specific inducer of ferroptosis. FIN56 induces ferroptosis by inducing degradation of GPX4. FIN56 also binds to and activates squalene synthase^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
LN-229	IC₅₀	4.2 μM Compound: 28; FIN56	Induction of ferroptosis in human LN-229 cells assessed as GPX4 degradation Induction of ferroptosis in human LN-229 cells assessed as GPX4 degradation	[PMID: 36332549]
U-118-MG	IC₅₀	2.6 μM Compound: 28; FIN56	Induction of ferroptosis in human U-118-MG cells assessed as GPX4 degradation Induction of ferroptosis in human U-118-MG cells assessed as GPX4 degradation	[PMID: 36332549]

In Vitro

FIN56 (5 μM) can trigger ferroptosis by inhibition of glutathione peroxidase 4 (GPX4)^[2].
FIN56 (5 μM, 10 hours) depletes GPX4 protein in HT-1080 cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	TP53^+/+ and TP53^-/- HCT116 cells
Concentration:	5 μM
Incubation Time:
Result:	Triggered ferroptosis.

Western Blot Analysis^[3]

Cell Line:	HT-1080 cells
Concentration:	5 μM
Incubation Time:	10 hours
Result:	Caused a large decrease in the abundance of GPX4 at the protein level.

Molecular Weight

517.66

Formula

C₂₅H₃₁N₃O₅S₂

CAS No.

1083162-61-1

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC(/C2=N\O)=C(C3=C2C=C(S(=O)(NC4CCCCC4)=O)C=C3)C=C1)(NC5CCCCC5)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (193.18 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9318 mL	9.6588 mL	19.3177 mL
5 mM	0.3864 mL	1.9318 mL	3.8635 mL
10 mM	0.1932 mL	0.9659 mL	1.9318 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.83 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.83 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.03%

Select Batch:

Data Sheet (276 KB) SDS (604 KB)

COA (250 KB) RP-HPLC (249 KB) LCMS (88 KB)

Handling Instructions (2659 KB)

References

[1]. Kenichi Shimada, et al. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis. Nat Chem Biol. 2016 Jul;12(7):497-503. [Content Brief]

[2]. Yangchun Xie, et al. The Tumor Suppressor p53 Limits Ferroptosis by Blocking DPP4 Activity. Cell Rep. 2017 Aug 15;20(7):1692-1704. [Content Brief]

[3]. Michael M Gaschler, et al. FINO₂ initiates ferroptosis through GPX4 inactivation and iron oxidation. Nat Chem Biol. 2018 May;14(5):507-515. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9318 mL	9.6588 mL	19.3177 mL	48.2942 mL
	5 mM	0.3864 mL	1.9318 mL	3.8635 mL	9.6588 mL
	10 mM	0.1932 mL	0.9659 mL	1.9318 mL	4.8294 mL
	15 mM	0.1288 mL	0.6439 mL	1.2878 mL	3.2196 mL
	20 mM	0.0966 mL	0.4829 mL	0.9659 mL	2.4147 mL
	25 mM	0.0773 mL	0.3864 mL	0.7727 mL	1.9318 mL
	30 mM	0.0644 mL	0.3220 mL	0.6439 mL	1.6098 mL
	40 mM	0.0483 mL	0.2415 mL	0.4829 mL	1.2074 mL
	50 mM	0.0386 mL	0.1932 mL	0.3864 mL	0.9659 mL
	60 mM	0.0322 mL	0.1610 mL	0.3220 mL	0.8049 mL
	80 mM	0.0241 mL	0.1207 mL	0.2415 mL	0.6037 mL
	100 mM	0.0193 mL	0.0966 mL	0.1932 mL	0.4829 mL

FIN56 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FIN561083162-61-1FIN 56FIN-56FerroptosisdegradationGPX4squalenesynthaseInhibitorinhibitorinhibit

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FIN56

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Other Forms of FIN56:

FIN56 Related Antibodies

24 Publications Citing Use of MCE FIN56

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Complete Stock Solution Preparation Table

FIN56 Related Classifications

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