FIN56
Based on 27 publication(s) in Google Scholar
FIN56 is a specific inducer of ferroptosis. FIN56 induces ferroptosis by inducing degradation of GPX4. FIN56 also binds to and activates squalene synthase.
For research use only. We do not sell to patients.
- Purity: 98.03%
- CAS No.: 1083162-61-1
- Formula: C25H31N3O5S2
- Molecular Weight:517.66
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Anti-Cancer Compound Library
HY-L025
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Ferroptosis Compound Library
HY-L051
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HY-L099
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Highly Selective Activators Library
HY-L159
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HY-L162
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HY-L181
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MCE Bioactive Compound Library
HY-L001V
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HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) FIN56
More- Nat Commun. 2024 Oct 26;15(1):9261. [Abstract]
- Redox Biol. 2021 Jan;38:101807. [Abstract]
- Mol Cell. 2026 Apr 16;86(8):1546-1559.e8. [Abstract]
- Mol Cell. 2023 Dec 7;83(23):4334-4351.e7. [Abstract]
- Sci Adv. 2026 May 29;12(22):eaeb2368. [Abstract]
- Sci Adv. 2024 Mar 29;10(13):eadk1200. [Abstract]
- Environ Sci Technol. 2026 Mar 3;60(8):6096-6110. [Abstract]
- Cell Death Discov. 2025 Sep 25;11(1):423. [Abstract]
- Dev Cell. 2024 Feb 26;59(4):517-528.e3. [Abstract]
- Sens Actuators B Chem. 2024 Nov 1.
- Free Radic Biol Med. 2024 Jun 1:S0891-5849(24)00506-9. [Abstract]
- Free Radic Biol Med. 2022 Jul:187:158-170. [Abstract]
- Mitochondrion. 2026 Mar:87:102112. [Abstract]
- Int Immunopharmacol. 2023 Jul:120:110291. [Abstract]
- Microchem J. 2025 Sep 9;218:115245.
- Microchem J. 2024 Dec.
- J Cell Mol Med. 2025 Jul;29(13):e70592. [Abstract]
- Cell Signal. 2025 Jan 3:111583. [Abstract]
- Med Oncol. 2023 Apr 10;40(5):141. [Abstract]
- Mol Carcinog. 2025 Jul 17. [Abstract]
- Mol Carcinog. 2023 Aug;62(8):1119-1135. [Abstract]
- Mol Biol Rep. 2025 Apr 21;52(1):407. [Abstract]
- Biochem Biophys Res Commun. 2024 Jul 23:733:150436. [Abstract]
- Res Sq. 2026 Jun 7.
- SSRN. 2025 Dec 2.
- Cornell University. 2025.
- Research Square Preprint. 2020 Jul.
Customer Validation & Images
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RT-PCR
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Cell Proliferation/Viability Assay
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
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IF
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| LN-229 | IC50 |
4.2 μM
Compound: 28; FIN56
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Induction of ferroptosis in human LN-229 cells assessed as GPX4 degradation
Induction of ferroptosis in human LN-229 cells assessed as GPX4 degradation
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[PMID: 36332549] |
| U-118-MG | IC50 |
2.6 μM
Compound: 28; FIN56
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Induction of ferroptosis in human U-118-MG cells assessed as GPX4 degradation
Induction of ferroptosis in human U-118-MG cells assessed as GPX4 degradation
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[PMID: 36332549] |
FIN56 (5 μM) can trigger ferroptosis by inhibition of glutathione peroxidase 4 (GPX4)[2].
FIN56 (5 μM, 10 hours) depletes GPX4 protein in HT-1080 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:TP53+/+ and TP53-/- HCT116 cells
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Concentration:5 μM
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Incubation Time:
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Result:Triggered ferroptosis.
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Cell Line:HT-1080 cells
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Concentration:5 μM
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Incubation Time:10 hours
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Result:Caused a large decrease in the abundance of GPX4 at the protein level.
Chemical Information
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CAS No. 1083162-61-1
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Appearance Solid
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Molecular Weight 517.66
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Formula C25H31N3O5S2
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Color White to off-white
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SMILES
O=S(C1=CC(/C2=N\O)=C(C3=C2C=C(S(=O)(NC4CCCCC4)=O)C=C3)C=C1)(NC5CCCCC5)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (27)
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Journal Impact Factor
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Most Recent
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Nat Commun
TNF inhibitors target a mevalonate metabolite/TRPM2/calcium signaling axis in neutrophils to dampen vasculitis in Behçet's disease. [Abstract]2024 Oct 26;15(1):9261. PMID: 39461948
FIN56 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Oct 26;15(1):9261. [Abstract]
BD-PMN was pretreated with FIN56 (1 μM and 5 μM) to activate FDFT1, for 16 hours. The expression levels of TNF, IL-6, IL-18, and IL-1β were detected by qRT-PCR (N = 4, for each group).
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Redox Biol
FBW7-NRA41-SCD1 axis synchronously regulates apoptosis and ferroptosis in pancreatic cancer cells. [Abstract]2021 Jan;38:101807. PMID: 33271455 -
Mol Cell
mTORC1 activity suppresses ferroptosis through a SCARB1-dependent HDL-tocopherol uptake pathway. [Abstract]2026 Apr 16;86(8):1546-1559.e8. PMID: 41997112 -
Mol Cell
EGFR promotes ALKBH5 nuclear retention to attenuate N6-methyladenosine and protect against ferroptosis in glioblastoma. [Abstract]2023 Dec 7;83(23):4334-4351.e7. PMID: 37979586 -
Sci Adv
2026 May 29;12(22):eaeb2368. PMID: 42213853 -
Sci Adv
2024 Mar 29;10(13):eadk1200. PMID: 38552012
FIN56 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Mar 29;10(13):eadk1200. [Abstract]
Cell viability assay of HT-1080 cells pretreated with or without AS for 1 h and then stimulated with 40 μM FIN56.
FIN56 purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Mar 29;10(13):eadk1200. [Abstract]
LDH release assay of HT-1080 cells pretreated with or without AS for 1 h and then stimulated with 40 μM FIN56.
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Environ Sci Technol
2026 Mar 3;60(8):6096-6110. PMID: 41700700 -
Cell Death Discov
Exploiting dysregulated iron homeostasis to eradicate persistent high-grade serous ovarian cancer. [Abstract]2025 Sep 25;11(1):423. PMID: 40998801 -
Dev Cell
A single fluorescent probe to examine the dynamics of mitochondria-lysosome interplay and extracellular vesicle role in ferroptosis. [Abstract]2024 Feb 26;59(4):517-528.e3. PMID: 38272028 -
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Free Radic Biol Med
Cannabidiol mitigates radiation-induced intestine ferroptosis via facilitating the heterodimerization of RUNX3 with CBFβ thereby promoting transactivation of GPX4. [Abstract]2024 Jun 1:S0891-5849(24)00506-9. PMID: 38830513
FIN56 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Jun 1:S0891-5849(24)00506-9. [Abstract]
FIN56 (20 μM). CBD represses γH2AX production and cell death caused by radiation and ferroptosis inducers. Representative live and dead cell stained by Calcein-AM/PI. Scale bar: 200 μm.
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Free Radic Biol Med
NAC alleviative ferroptosis in diabetic nephropathy via maintaining mitochondrial redox homeostasis through activating SIRT3-SOD2/Gpx4 pathway. [Abstract]2022 Jul:187:158-170. PMID: 35660452
FIN56 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2022 Jul:187:158-170. [Abstract]
FIN56. Effect of NAC and/or on Gpx4 expression and ferroptosis in MDCK cells under HG treatment. Immunofluorescence staining for Gpx4 (scale bar is 75 μm).
FIN56 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2022 Jul:187:158-170. [Abstract]
FIN56. Effect of NAC and/or on Gpx4 expression and ferroptosis in MDCK cells under HG treatment. Representative blots of CD71 and Slc7a11.
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Mitochondrion
Sensitivity of primary mitochondrial disease fibroblasts to ferroptosis: The role of intracellular iron. [Abstract]2026 Mar:87:102112. PMID: 41581630 -
Int Immunopharmacol
Cisatracurium besylate rescues Mycobacterium Tuberculosis-infected macrophages from necroptosis and enhances the bactericidal effect of isoniazid. [Abstract]2023 Jul:120:110291. PMID: 37182451 -
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J Cell Mol Med
2025 Jul;29(13):e70592. PMID: 40576283 -
Cell Signal
HDAC inhibitor enhances ferroptosis susceptibility of AML cells by stimulating iron metabolism. [Abstract]2025 Jan 3:111583. PMID: 39756501 -
Med Oncol
2023 Apr 10;40(5):141. PMID: 37036615 -
Mol Carcinog
Pregnane X Receptor Activation Induces Ferroptosis Resistance Through Upregulation of FSP1. [Abstract]2025 Jul 17. PMID: 40674609 -
Mol Carcinog
PRMT1 inhibition promotes ferroptosis sensitivity via ACSL1 upregulation in acute myeloid leukemia. [Abstract]2023 Aug;62(8):1119-1135. PMID: 37144835 -
Mol Biol Rep
2025 Apr 21;52(1):407. PMID: 40257700 -
Biochem Biophys Res Commun
2024 Jul 23:733:150436. PMID: 39053102 -
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Solvent & Solubility
DMSO : ≥ 100 mg/mL (193.18 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.83 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.83 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (604 KB)
- English - EN (604 KB)
- Français - FR (604 KB)
- Deutsch - DE (604 KB)
- Norwegian - NO (604 KB)
- Español - ES (604 KB)
- Swedish - SV (604 KB)
- Italian - IT (604 KB)
- Korean - KR (604 KB)
- Portuguese - PT (604 KB)
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Handling Instructions (2659 KB)
References
[1]. Kenichi Shimada, et al. Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis. Nat Chem Biol. 2016 Jul;12(7):497-503. [Content Brief]
[2]. Yangchun Xie, et al. The Tumor Suppressor p53 Limits Ferroptosis by Blocking DPP4 Activity. Cell Rep. 2017 Aug 15;20(7):1692-1704. [Content Brief]
[3]. Michael M Gaschler, et al. FINO2 initiates ferroptosis through GPX4 inactivation and iron oxidation. Nat Chem Biol. 2018 May;14(5):507-515. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9318 mL | 9.6588 mL | 19.3177 mL | 48.2942 mL |
| 5 mM | 0.3864 mL | 1.9318 mL | 3.8635 mL | 9.6588 mL | |
| 10 mM | 0.1932 mL | 0.9659 mL | 1.9318 mL | 4.8294 mL | |
| 15 mM | 0.1288 mL | 0.6439 mL | 1.2878 mL | 3.2196 mL | |
| 20 mM | 0.0966 mL | 0.4829 mL | 0.9659 mL | 2.4147 mL | |
| 25 mM | 0.0773 mL | 0.3864 mL | 0.7727 mL | 1.9318 mL | |
| 30 mM | 0.0644 mL | 0.3220 mL | 0.6439 mL | 1.6098 mL | |
| 40 mM | 0.0483 mL | 0.2415 mL | 0.4829 mL | 1.2074 mL | |
| 50 mM | 0.0386 mL | 0.1932 mL | 0.3864 mL | 0.9659 mL | |
| 60 mM | 0.0322 mL | 0.1610 mL | 0.3220 mL | 0.8049 mL | |
| 80 mM | 0.0241 mL | 0.1207 mL | 0.2415 mL | 0.6037 mL | |
| 100 mM | 0.0193 mL | 0.0966 mL | 0.1932 mL | 0.4829 mL |
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