miR-26a-Targeting SLC7A11 Regulates Erastin-Induced Granulosa Cell Ferroptosis

  • Antioxidants (Basel). 2025 Oct 26;14(11):1283. doi: 10.3390/antiox14111283.
Xue Zhao  1  2  3  4 ,  Yuheng Pan  2  3  4 ,  Shuang Liang  2  3  4 ,  Yuhang Lei  2  3  4 ,  Yan Wang  2  3  4 ,  Lei Chen  2  3  4 ,  Ye Zhao  2  3  4 ,  Mailin Gan  2  3  4 ,  Linyuan Shen  2  3  4 ,  Xin Yang  1 ,  Li Zhu  2  3  4
Affiliations
  • 1. Animal Disease Prevention and Green Development Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610065, China.
  • 2. Farm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
  • 3. State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
  • 4. Key Laboratory of Livestock and Poultry Multiomics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, China.
PMID: 41300440 DOI: 10.3390/antiox14111283
Abstract

Granulosa cell Ferroptosis is a critical factor in follicular atresia and premature ovarian insufficiency (POI). As a regulated form of programmed cell death, Ferroptosis is gaining significant attention in reproductive medicine research. MicroRNAs (miRNAs) play a crucial role in regulating key aspects of Ferroptosis, including the glutathione-GPX4 pathway, glutamate/cystine transport, and iron and lipid metabolism. The present study demonstrates that miR-26a positively modulates Ferroptosis by targeting SLC7A11, a member of the solute carrier family. We found that oocytes and granulosa cells are susceptible to the Ferroptosis inducer erastin, and employed RNA Sequencing to delineate the miRNA expression profiles during erastin-induced damage and Ferroptosis. Notably, miR-26a expression was significantly upregulated in erastin-treated oocytes. Importantly, overexpression of miR-26a promoted Ferroptosis in granulosa cells, while its knockdown inhibited this process. Ectopic miR-26a expression suppressed SLC7A11, thereby increasing Ferroptosis. Our findings indicate that miR-26a influences Ferroptosis by inhibiting glutathione synthesis, reducing cellular antioxidant capacity, and suggesting a potential strategy to enhance reproductive potential.

Keywords
ferroptosis inducer; granulosa cells; miRNA; mitochondria; oxidantive stress.
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