Beta Tubulin Antibody (YA585)

MedchemExpress Validation
Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg), Jurkat (lane 4(20μg) using Beta Tubulin (HY-P80487) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80487, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

MedchemExpress Validation
Immunocytochemistry analysis of NIH3T3 cells labeling Beta Tubulin with Beta Tubulin antibody (HY-P80487) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Beta Tubulin antibody (HY-P80487) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

MedchemExpress Validation
Immunocytochemistry analysis of Hela cells labeling Beta Tubulin with Beta Tubulin (HY-P80487) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Beta Tubulin antibody (HY-P80487) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded Rat kdiney tissue using Beta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80487, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded Rat kdiney tissue using Beta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80487, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

MedchemExpress Validation
Flow cytometric analysis of 1X10^6 NIH3T3 cells labeling Beta Tubulin Antibody(HY-P80487, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Beta Tubulin Antibody (YA585) purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Nov 17;15(1):40227. [Abstract]

Customer Validation
The housekeeping protein β-Tubulin (Cat. # HY-P80487) diluted 1:1,000 in TBSM were used as primary antibodies (MedChemExpress) and incubated with the membrane overnight at 4℃. After three 5 min washes with TBS, goat anti-rabbit IgG diluted 1:5,000 in TBSM was used as secondary antibody and incubated with the membrane for 30 min.

Beta Tubulin Antibody (YA585) purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2025 Jan 22:495:117237. [Abstract]

Customer Validation
The collected mitochondrial proteins (20 μg) were separated using 12% gel electrophoresis and transferred to polyvinylidene fluoride (PVDF) membranes. The membranes were blocked with 5% milk for 1.5 h at 37 ℃ and incubated with primary antibodies overnight at 4 ℃. Beta Tubulin Antibody (YA585) (1:2000).