CLEC12A Antibody (YA4328)

Cat. No.: HY-P84631: Data Sheet User Guide for Antibodies
FAQs
Technical Support

CLEC12A Antibody (YA4328) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CLEC12A.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

Synthetic products have potential research and development risk.

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CLEC12A Antibody (YA4328) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CLEC12A.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 30.8 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q5QGZ9

Gene ID

160364 [NCBI]

Immunogen

Purified recombinant fragment of human CLL1 (AA: extra 65-265) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:500
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Myeloid inhibitory C-type lectin receptor that acts as a negative regulator of myeloid cell activation (PubMed:14739280, PubMed:15238421, PubMed:16239426, PubMed:34234773, PubMed:38367667, PubMed:38386511, PubMed:39143217). Myeloid cell inhibition is required to limit proinflammatory pathways and protect against excessive inflammation (By similarity). Specifically recognizes and binds various structures, such as neutrophil extracellular traps (NETs) or monosodium urate crystals (PubMed:38367667, PubMed:38386511, PubMed:39143217). Also acts as a pattern-recognition receptor for pathogen-associated molecules, such as plasmodium hemozoin or mycobacterial micolic acid (PubMed:31269448, PubMed:36542980). Ligand-binding induces phosphorylation of its ITIM motif, followed by recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11, which counteract tyrosine-protein kinase SYK, thereby preventing myeloid cell activation (PubMed:14739280, PubMed:16239426, PubMed:34234773). Acts as a pattern-recognition receptor for NETs in neutrophils: specifically recognizes DNA in NETs, leading to inhibit neutrophil activation and limit further NET formation (PubMed:39143217). This regulation is essential for controlling key neutrophil responses and limit NET-mediated inflammatory conditions (By similarity). Also recognizes dead cells by acting as a receptor for monosodium urate crystals, leading to down-regulate neutrophil activation (PubMed:38367667, PubMed:38386511). Binding to monosodium urate crystals also promotes the type I interferon response (By similarity). Acts as an inhibitor of natural killer (NK) cell cytotoxicity (PubMed:15238421). Also acts as an ihibitor of dendritic cell maturation in an IL10-dependent manner (PubMed:16239426)

Subcellular Localization：Cell membrane; Single-pass type II membrane protein

Expression：
Tissue_specificity:It is primarily expressed in lymphoid tissues and immune cells, including natural killer (NK) cells, T cells, dendritic cells, and monocytes or macrophages (PubMed:14739280, PubMed:15238421, PubMed:15548716, PubMed:16239426, PubMed:16838277) . It can be detected (at the protein level) in macrophage-rich red pulp and lymph nodes in the spleen (PubMed:16838277) . It can also be detected in peripheral blood leukocytes, dendritic cells, bone marrow, monocytes, mononuclear leukocytes, and macrophages (PubMed:16838277) .

Induction:Down-regulated in activated leukocytes recruited to a site of inflammation

Isoforms & Post-Translational Modification：Q5QGZ9 has 5 isomers: Q5QGZ9-2: 30762 Da (predicted); Q5QGZ9-1: 32040 Da (predicted); Q5QGZ9-3: 8965 Da (predicted); Q5QGZ9-4: 27276 Da (predicted); Q5QGZ9-5: 24449 Da (predicted).
Phosphorylated at Tyr-7 by SRC in the ITIM motif following ligand-binding, promoting recruitment of tyrosine-protein phosphatases PTPN6 and PTPN11;Highly N-glycosylated; glycosylation varies between cell types

Subunit：Homodimer; disulfide-linked (By similarity). Interacts (when the ITIM motif is phosphorylated) with PTPN6 and PTPN11 (PubMed:14739280)

Synonyms

CLEC12A; MICL; CD371; CLL-1; DCAL-2

Documentation

User Guide for Antibodies (1077 KB)