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VCAM1 Antibody (YA3852)

(Synonyms: VCAM1; INCAM-100)
Customer Review

Based on 1 Customer Validation

VCAM1 Antibody (YA3852) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VCAM1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Rat, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL WB FC ICC
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • VCAM1 Antibody (YA3852) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

VCAM1 Antibody (YA3852) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VCAM1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Rat, Monkey
  • Observed Molecular Weight
    Observed band size: 110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 81 kDa
Immunogen

Purified recombinant fragment of human CD106 (AA: extra 25-183) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718849

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for VCAM1 Antibody (YA3852)
    Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), C2C12 (lane 3(20μg), HUVEC (lane 4(20μg), HT-29 (lane 5(20μg) using VCAM1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
    Flow cytometric analysis of 1X106 K562 cells labeling VCAM1 Antibody (HY-P84155, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/300 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
    Immunocytochemistry analysis of Hela cells labeling VCAM1 with VCAM1 Antibody (HY-P84155) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VCAM1 Antibody (HY-P84155) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
    Immunocytochemistry analysis of Hela cells labeling VCAM1 with VCAM1 Antibody (HY-P84155) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VCAM1 Antibody (HY-P84155) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    VCAM1 cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Secreted

  • Expression


    Tissue_specificity:It is expressed on inflammatory vascular endothelial cells, as well as macrophage-like cells and dendritic cells in normal and inflammatory tissues.

    Induction:By pro-inflammatory cytokines, including TNFalpha, and also by ROS, oxidized low density lipoprotein, high glucose concentration, toll-like receptor agonists, and shear stress

  • Isoforms & Post-Translational Modification

    P19320 has 3 isomers: P19320-1: 81276 Da (predicted); P19320-2: 71255 Da (predicted); P19320-3: 74346 Da (predicted).
    Cleaved by the metalloproteinase ADAM17 to generate the soluble form;Sialoglycoprotein;Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination; leading to proteasomal degradation

  • SwissProt ID

    P19320

  • Gene ID
    7412 [NCBI]
  • Synonyms

    VCAM1; INCAM-100

VCAM1 Antibody (YA3852) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), C2C12 (lane 3(20μg), HUVEC (lane 4(20μg), HT-29 (lane 5(20μg) using VCAM1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling VCAM1 Antibody (HY-P84155, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/300 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling VCAM1 with VCAM1 Antibody (HY-P84155) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VCAM1 Antibody (HY-P84155) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling VCAM1 with VCAM1 Antibody (HY-P84155) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VCAM1 Antibody (HY-P84155) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VCAM1 Antibody (YA3852) Product Image
    1/16
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • VCAM1 Antibody (YA3852) Product Image
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), C2C12 (lane 3(20μg), HUVEC (lane 4(20μg), HT-29 (lane 5(20μg) using VCAM1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling VCAM1 Antibody (HY-P84155, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/300 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling VCAM1 with VCAM1 Antibody (HY-P84155) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VCAM1 Antibody (HY-P84155) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling VCAM1 with VCAM1 Antibody (HY-P84155) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VCAM1 Antibody (HY-P84155) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • Experimental Validation Results for VCAM1 Antibody (YA3852)
  • VCAM1 Antibody (YA3852) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), C2C12 (lane 3(20μg), HUVEC (lane 4(20μg), HT-29 (lane 5(20μg) using VCAM1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • VCAM1 Antibody (YA3852) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 K562 cells labeling VCAM1 Antibody (HY-P84155, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/300 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • VCAM1 Antibody (YA3852) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling VCAM1 with VCAM1 Antibody (HY-P84155) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VCAM1 Antibody (HY-P84155) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VCAM1 Antibody (YA3852) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling VCAM1 with VCAM1 Antibody (HY-P84155) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with VCAM1 Antibody (HY-P84155) at 1/500 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • VCAM1 Antibody (YA3852) Product Image WB
  • VCAM1 Antibody (YA3852) Product Image FC
  • VCAM1 Antibody (YA3852) Product Image ICC
  • VCAM1 Antibody (YA3852) Product Image ICC