IKB alpha Antibody (YA6438)(PBS only)

Cat. No.: HY-P86746A: Data Sheet User Guide for Antibodies
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IKB alpha Antibody (YA6438) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IKB alpha.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

IKB alpha Antibody (YA6438) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to IKB alpha.

Host

Mouse

Molecular Weight

Predicted band size: 36 kDa

Species Reactivity

Human, Rat

SwissProt ID

P25963

Gene ID

4792 [NCBI]

Immunogen

Recombinant human IKB alpha Protein aa 1-77/314

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466)

Subcellular Localization：Cytoplasm,Nucleus

Expression：Induction: Induced in adherent monocytes

Isoforms & Post-Translational Modification：P25963: 317 amino acids, molecular weight 35609 Da.

Subunit：Interacts with RELA; the interaction requires the nuclear import signal (PubMed:1493333)

Synonyms

NFKBIA; Inhibitor of KB alpha; I kappa B alpha; I(Kappa)B(alpha); IkappaBalpha; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer binding protein MAD3; NF kappa B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha; IKBA_HUMAN.

Documentation

User Guide for Antibodies (1077 KB)