Phospho-Hormone Sensitive Lipase (Ser855) Antibody (YA2599)(PBS only)

Cat. No.: HY-P82854A: Data Sheet User Guide for Antibodies
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Phospho-Hormone Sensitive Lipase (Ser855) Antibody (YA2599) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Hormone Sensitive Lipase (Ser855).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Phospho-Hormone Sensitive Lipase (Ser855) Antibody (YA2599) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Hormone Sensitive Lipase (Ser855).

Host

Rabbit

Molecular Weight

Predicted band size: 117 kDa;

Observed band size: 83 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q05469

Gene ID

3991 [NCBI]

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser855 of human Hormone sensitive lipase/HSL aa849-862.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed:15716583, PubMed:15955102, PubMed:19800417, PubMed:8812477). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs (PubMed:19800417). Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity)

Subcellular Localization：Cell membrane; Membrane, caveola; Cytoplasm, cytosol; Lipid droplet

Expression：
Tissue_specificity:testis

Isoforms & Post-Translational Modification：Q05469 has 2 isomers: Q05469-1: 116598 Da (predicted); Q05469-2: 84128 Da (predicted).
Phosphorylation by AMPK reduces its translocation towards the lipid droplets

Subunit：Monomer and homodimer (By similarity). Interacts with CAVIN1 in the adipocyte cytoplasm (PubMed:17026959). Interacts with PLIN5 (By similarity)

Research Field

Cardiovascular

Synonyms

LIPE; Hormone-sensitive lipase; HSL

Documentation

User Guide for Antibodies (1077 KB)