Syntenin-1 Antibody (YA5162)

Cat. No.: HY-P85470: Data Sheet User Guide for Antibodies
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Syntenin-1 Antibody (YA5162) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Syntenin-1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Syntenin-1 Antibody (YA5162) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Syntenin-1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 32 kDa;

Species Reactivity

Human, Rat, Dog, Pig

SwissProt ID

O00560

Gene ID

6386 [NCBI]

Immunogen

Purified recombinant human Syntenin-1 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Multifunctional adapter protein involved in diverse array of functions including trafficking of transmembrane proteins, neuro and immunomodulation, exosome biogenesis, and tumorigenesis (PubMed:26291527). Positively regulates TGFB1-mediated SMAD2/3 activation and TGFB1-induced epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. May increase TGFB1 signaling by enhancing cell-surface expression of TGFR1 by preventing the interaction between TGFR1 and CAV1 and subsequent CAV1-dependent internalization and degradation of TGFR1 (PubMed:25893292). In concert with SDC1/4 and PDCD6IP, regulates exosome biogenesis (PubMed:22660413). Regulates migration, growth, proliferation, and cell cycle progression in a variety of cancer types (PubMed:26539120). In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA) (PubMed:11498591). May also play a role in vesicular trafficking (PubMed:11179419). Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway (PubMed:10230395)

Subcellular Localization：Cell junction, focal adhesion; Cell junction, adherens junction; Cell membrane; Peripheral membrane protein; Endoplasmic reticulum membrane; Peripheral membrane protein; Nucleus; Melanosome; Cytoplasm, cytosol; Cytoplasm, cytoskeleton; Secreted, extracellular exosome; Membrane raft

Expression：
Tissue_specificity:Expressed in lung cancer, including adenocarcinoma, squamous cell carcinoma, and small cell carcinoma (protein level) (PubMed: 25893292) . Widely expressed. Expressed in fetal kidney, liver, lung, and brain. In humans, the highest expression is found in the heart and placenta.

Induction:By IFNG/IFN-gamma in melanoma cells

Isoforms & Post-Translational Modification：O00560 has 3 isomers: O00560-1: 32444 Da (predicted); O00560-2: 32316 Da (predicted); O00560-3: 31762 Da (predicted).
Phosphorylated on tyrosine residues

Subunit：Monomer and homodimer (By similarity). Interacts with SDC1, SDC2, SDC3, SDC4, NRXN2, EPHA7, EPHB1, NF2 isoform 1, TGFA and IL5RA. Interacts with NFASC and PTPRJ (By similarity). Interacts with SDCBP2 (PubMed:11152476). Interacts with PDCD6IP (PubMed:22660413). Forms a complex with PDCD6IP and SDC2 (PubMed:22660413). Interacts (via C-terminus) with TGFBR1 (PubMed:25893292). Binds to FZD7; this interaction is increased by inositol trisphosphate (IP3) (PubMed:27386966). Interacts with SMO (By similarity)

Synonyms

SDCBP; MDA9; SYCL; Syntenin-1; Melanoma differentiation-associated protein 9; MDA-9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; TACIP18; Scaffold protein Pbp1; Syndecan-binding protein 1

Documentation

User Guide for Antibodies (1077 KB)