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CYP7A1 Antibody (YA5117)

(Synonyms: CYP7A1; CYP7; Cholesterol 7-alpha-monooxygenase; CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1)
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CYP7A1 Antibody (YA5117) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CYP7A1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    WB

  • Reactivity :

    Human, Golden hamster

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • CYP7A1 Antibody (YA5117) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:2000

Product Details

Description

CYP7A1 Antibody (YA5117) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CYP7A1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Golden hamster
  • Calculated Molecular Weight Predicted band size: 58 kDa;
Immunogen

Purified recombinant human CYP7A1 (C-terminus) protein fragments expressed in E.coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

RRID

AB_3719024

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    CYP7A1 is an A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives[1][2][3][4][5].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass membrane protein; Microsome membrane; Single-pass membrane protein

  • Expression


    Tissue_specificity:Detected in liver

    Induction:Up-regulated by glucose and by cholestyramine. Down-regulated by chenodeoxycholic acid

  • SwissProt ID

    P22680

  • Gene ID
    1581 [NCBI]
  • Synonyms

    CYP7A1; CYP7; Cholesterol 7-alpha-monooxygenase; CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1

CYP7A1 Antibody (YA5117) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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