EGF Antibody (YA4822)

Cat. No.: HY-P85130: Data Sheet User Guide for Antibodies
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EGF Antibody (YA4822) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to EGF.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

EGF Antibody (YA4822) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to EGF.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 134 kDa;

Species Reactivity

Human

SwissProt ID

P01133

Gene ID

1950 [NCBI]

Immunogen

Purified recombinant fragment of human EGF expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941)

Subcellular Localization：Membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:It is expressed in the kidneys, salivary glands, brain, and prostate.

Isoforms & Post-Translational Modification：P01133 has 3 isomers: P01133-1: 133994 Da (predicted); P01133-2: 129256 Da (predicted); P01133-3: 129746 Da (predicted).
O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor)

Subunit：Interacts with EGFR and promotes EGFR dimerization. Interacts with RHBDF2 (By similarity).

Synonyms

EGF; Pro-epidermal growth factor; EGF

Documentation

User Guide for Antibodies (1077 KB)