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Cytochrome P450 2S1 Antibody

(Synonyms: UNQ891/PRO1906, CYP2S1, Cytochrome P450 2S1, CYPIIS1, Hydroperoxy icosatetraenoate dehydratase, Thromboxane-A synthase)

Cytochrome P450 2S1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Cytochrome P450 2S1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cytochrome P450 2S1 Antibody
  • Cytochrome P450 2S1 Antibody Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-2000 1:100-200 1:100-500

Product Details

Description

Cytochrome P450 2S1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Cytochrome P450 2S1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 62 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Immunogen

Synthetic peptide corresponding to the center region of human Cytochrome P450 2S1.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cytochrome P450 2S1 Antibody
    Western blot analysis of extracts from Hela (lane 2, 40ug), A549 (lane 3, 40ug) and HUVEC (lane 3, 40ug) using Cytochrome P450 2S1 Antibody (HY-P811060). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Cytochrome P450 2S1 is an A cytochrome P450 monooxygenase involved in the metabolism of retinoids and eicosanoids. In epidermis, may contribute to the oxidative metabolism of all-trans-retinoic acid. For this activity, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Additionally, displays peroxidase and isomerase activities toward various oxygenated eicosanoids such as prostaglandin H2 (PGH2) and hydroperoxyeicosatetraenoates (HPETEs). Independently of cytochrome P450 reductase, NADPH, and O2, catalyzes the breakdown of PGH2 to hydroxyheptadecatrienoic acid (HHT) and malondialdehyde (MDA), which is known to act as a mediator of DNA damage[1][2].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Microsome membrane

  • Expression


    Tissue_Specificity: Expressed at higher levels in extrahepatic tissues including trachea, lung, stomach, small intestine, colon, kidney, breast, placenta and spleen. Expressed in peripheral blood leukocytes. Constitutively expressed in skin (at protein level).
    Induction: Up-regulated in skin upon exposure to ultraviolet radiation or treatment with all-trans retinoic acid (substrate-inducible).

  • Isoforms & Post-Translational Modification

    Cytochrome P450 2S1 has 2 isoforms, Q96SQ9-1: amino acid length is 504, molecular weight is 55817 Da (predicted); Q96SQ9-2: amino acid length is 564, molecular weight is 62231 Da (predicted).

  • SwissProt ID

    Q96SQ9

  • Gene ID
    29785 [NCBI]
  • Synonyms

    UNQ891/PRO1906, CYP2S1, Cytochrome P450 2S1, CYPIIS1, Hydroperoxy icosatetraenoate dehydratase, Thromboxane-A synthase

References

[1]. Smith G, et al. Cutaneous expression of cytochrome P450 CYP2S1: individuality in regulation by therapeutic agents for psoriasis and other skin diseases. Lancet. 2003 Apr 19;361(9366):1336-43. [Content Brief]

[2]. Bui P, et al. Human CYP2S1 metabolizes cyclooxygenase- and lipoxygenase-derived eicosanoids. Drug Metab Dispos. 2011 Feb;39(2):180-90. [Content Brief]

Cytochrome P450 2S1 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 40ug), A549 (lane 3, 40ug) and HUVEC (lane 3, 40ug) using Cytochrome P450 2S1 Antibody (HY-P811060). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Cytochrome P450 2S1 Antibody Product Image
    1/16
  • Experimental Validation Results for Cytochrome P450 2S1 Antibody
  • Cytochrome P450 2S1 Antibody Product Image
  • Experimental Validation Results for Cytochrome P450 2S1 Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 40ug), A549 (lane 3, 40ug) and HUVEC (lane 3, 40ug) using Cytochrome P450 2S1 Antibody (HY-P811060). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cytochrome P450 2S1 Antibody
  • Cytochrome P450 2S1 Antibody Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2, 40ug), A549 (lane 3, 40ug) and HUVEC (lane 3, 40ug) using Cytochrome P450 2S1 Antibody (HY-P811060). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Cytochrome P450 2S1 Antibody Product Image WB