SLC9A1 Antibody (YA10027)
(Synonyms: APNH1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1, APNH, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1)SLC9A1 Antibody (YA10027) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to SLC9A1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:1000-2000 |
Product Details
SLC9A1 Antibody (YA10027) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to SLC9A1.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 91 kDa
Synthetic peptide corresponding to human SLC9A1 protein.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA, and 0.05% Proclin300.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SLC9A1 is an Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism. Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry (By similarity). Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival. In addition, can transport lithium Li(+) and also functions as a Na(+)/Li(+) antiporter. SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].
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Subcellular Localization
Cell membrane; Basolateral cell membrane
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Expression
Tissue_Specificity: Kidney and intestine. -
Isoforms & Post-Translational Modification
SLC9A1 has 2 isoforms, P19634-1: amino acid length is 815, molecular weight is 90763 Da (predicted); P19634-2: amino acid length is 555, molecular weight is 61013 Da (predicted).O-glycosylated
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Subunit
Homodimer; dimerization is crucial for its function.
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SwissProt ID
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Synonyms
APNH1, NHE1, SLC9A1, Sodium/hydrogen exchanger 1, APNH, Na(+)/H(+) exchanger 1, Solute carrier family 9 member 1, NHE-1
Documentation
References
[1]. Pang T, et al. Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers. J Biol Chem. 2001 May 18;276(20):17367-72. [Content Brief]
[2]. Murtazina R, et al. Functional analysis of polar amino-acid residues in membrane associated regions of the NHE1 isoform of the mammalian Na+/H+ exchanger. Eur J Biochem. 2001 Sep;268(17):4674-85. [Content Brief]
[3]. Slepkov ER, et al. Proline residues in transmembrane segment IV are critical for activity, expression and targeting of the Na+/H+ exchanger isoform 1. Biochem J. 2004 Apr 1;379(Pt 1):31-8. [Content Brief]
[4]. Pang T, et al. Role of calcineurin B homologous protein in pH regulation by the Na+/H+ exchanger 1: tightly bound Ca2+ ions as important structural elements. Biochemistry. 2004 Mar 30;43(12):3628-36. [Content Brief]
[5]. Slepkov ER, et al. Structural and functional characterization of transmembrane segment IV of the NHE1 isoform of the Na+/H+ exchanger. J Biol Chem. 2005 May 6;280(18):17863-72. [Content Brief]
[6]. Hisamitsu T, et al. Dimerization is crucial for the function of the Na+/H+ exchanger NHE1. Biochemistry. 2006 Nov 7;45(44):13346-55. [Content Brief]
[7]. Pedersen SF, et al. NHE1 inhibition by amiloride- and benzoylguanidine-type compounds. Inhibitor binding loci deduced from chimeras of NHE1 homologues with endogenous differences in inhibitor sensitivity. J Biol Chem. 2007 Jul 6;282(27):19716-27. [Content Brief]
[8]. Abu Jawdeh BG, et al. Phosphoinositide binding differentially regulates NHE1 Na+/H+ exchanger-dependent proximal tubule cell survival. J Biol Chem. 2011 Dec 9;286(49):42435-42445. [Content Brief]
[9]. Webb BA, et al. A Histidine Cluster in the Cytoplasmic Domain of the Na-H Exchanger NHE1 Confers pH-sensitive Phospholipid Binding and Regulates Transporter Activity. J Biol Chem. 2016 Nov 11;291(46):24096-24104. [Content Brief]
[10]. Li X, et al. Acidic residues of extracellular loop 3 of the Na(+)/H(+) exchanger type 1 are important in cation transport. Mol Cell Biochem. 2020 May;468(1-2):13-20. [Content Brief]
[11]. Aronson PS, et al. Modifier role of internal H+ in activating the Na+-H+ exchanger in renal microvillus membrane vesicles. Nature. 1982 Sep 9;299(5879):161-3. [Content Brief]
[12]. Busch S, et al. Expression of the human sodium/proton exchanger NHE-1 in Xenopus laevis oocytes enhances sodium/proton exchange activity and establishes sodium/lithium countertransport. Pflugers Arch. 1995 Apr;429(6):859-69. [Content Brief]
[13]. Putney LK, et al. Na-H exchange-dependent increase in intracellular pH times G2/M entry and transition. J Biol Chem. 2003 Nov 7;278(45):44645-9. [Content Brief]
[14]. Wu KL, et al. The NHE1 Na+/H+ exchanger recruits ezrin/radixin/moesin proteins to regulate Akt-dependent cell survival. J Biol Chem. 2004 Jun 18;279(25):26280-6. [Content Brief]
[15]. Lin X, et al. A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12631-6. [Content Brief]
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