P2RX7 Antibody (YA4449)
(Synonyms: P2X7)Based on 1 publication(s) in Google Scholar
P2RX7 Antibody (YA4449) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to P2RX7.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
WB, IHC-P, FC, ELISA
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Reactivity :
Human, Mouse, Rabbit
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
Publications Citing Use of MedChemExpress (MCE) P2RX7 Antibody (YA4449)
More
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
P2RX7 Antibody (YA4449) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to P2RX7.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rabbit
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Observed Molecular WeightObserved band size: 70-80 kDaNote: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 69 kDa
Purified recombinant fragment of human P2RX7 (AA: 226-452) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.05% sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Publications (1)
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Journal Impact Factor
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Most Recent
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Int Immunopharmacol
CGRP alleviates epilepsy via JAK1-STAT1-P2RX7 signaling: a novel neuroprotective axis targeting neuronal damage. [Abstract]2026 Feb 1:170:116080. PMID: 41429063
Verification Images
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Western blot analysis of extracts from A431(lane 1(40μg) )
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Western blot analysis of extracts from A431(lane 1(40μg) ), U251(lane 2(40μg) ),Hela(lane 3(40μg) ), U937(lane 4(40μg) )and HepG2(lane 5(40μg) ) using P2RX7 antibody(31128P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH,20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human endometrium tissue using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human heart tissue using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast tissue using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast cancer using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
P2RX7 is an ATP-gated nonselective transmembrane cation channel that requires high millimolar concentrations of ATP for activation. Upon ATP binding, it rapidly opens to allow the influx of small cations Na(+) and Ca(2+), and the K(+) efflux. Also has the ability to form a large pore in the cell membrane, allowing the passage of large cationic molecules. In microglia, may mediate NADPH transport across the plasma membrane. In immune cells, P2RX7 acts as a molecular sensor in pathological inflammatory states by detecting and responding to high local concentrations of extracellar ATP. In microglial cells, P2RX7 activation leads to the release of pro-inflammatory cytokines, such as IL-1beta and IL-18, through the activation of the NLRP3 inflammasome and caspase-1. Cooperates with KCNK6 to activate NLRP3 inflammasome. Activates death pathways leading to apoptosis and autophagy. Activates death pathways leading to pyroptosis; Shows ion channel activity but no macropore function; Non-functional channel; Non-functional channel[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Cell membrane; Multi-pass membrane protein
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Expression
Tissue_specificity:Widely expressed with highest levels in brain and immune tissues; Predominant form in many tissues -
Isoforms & Post-Translational Modification
Q99572 has 9 isomers: Q99572-1: 68585 Da (predicted); Q99572-2: 41792 Da (predicted); Q99572-3: 14749 Da (predicted); Q99572-4: 49165 Da (predicted); Q99572-5: 31294 Da (predicted); Q99572-6: 35544 Da (predicted); Q99572-7: 31446 Da (predicted); Q99572-8: 58239 Da (predicted); Q99572-9: 29338 Da (predicted).
Phosphorylation results in its inactivation;ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel;Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface (PubMed:18971257). Palmitoylation prevents channel desensitization by physically anchoring the palmitoylated groups to the membrane (By similarity) -
Subunit
Homotrimers (PubMed:20453110). Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB (PubMed:11707406). Interacts (via C-terminus) with EMP2 (PubMed:12107182). Interacts with isoform B; this interaction potentiates P2RX7 responses (PubMed:20453110)
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SwissProt ID
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Synonyms
P2X7
Documentation
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Data Sheet (259 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)