P2RX7 Antibody (YA4449)

(Synonyms: P2X7)
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Based on 1 publication(s) in Google Scholar

P2RX7 Antibody (YA4449) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to P2RX7.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rabbit

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated

Publications Citing Use of MedChemExpress (MCE) P2RX7 Antibody (YA4449)

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

P2RX7 Antibody (YA4449) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to P2RX7.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rabbit
  • Observed Molecular Weight
    Observed band size: 70-80 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 69 kDa
Immunogen

Purified recombinant fragment of human P2RX7 (AA: 226-452) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

RRID

AB_3718978

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for P2RX7 Antibody (YA4449)
    Western blot analysis of extracts from A431(lane 1(40μg) )
  • Experimental Validation Results for P2RX7 Antibody (YA4449)
    Western blot analysis of extracts from A431(lane 1(40μg) ), U251(lane 2(40μg) ),Hela(lane 3(40μg) ), U937(lane 4(40μg) )and HepG2(lane 5(40μg) ) using P2RX7 antibody(31128P). Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH,20035, 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for P2RX7 Antibody (YA4449)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for P2RX7 Antibody (YA4449)
    Immunohistochemical analysis of paraffin-embedded human heart tissue using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for P2RX7 Antibody (YA4449)
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for P2RX7 Antibody (YA4449)
    Immunohistochemical analysis of paraffin-embedded human breast tissue using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for P2RX7 Antibody (YA4449)
    Immunohistochemical analysis of paraffin-embedded human breast cancer using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for P2RX7 Antibody (YA4449)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using P2RX7 Antibody (HY-P84752, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    P2RX7 is an ATP-gated nonselective transmembrane cation channel that requires high millimolar concentrations of ATP for activation. Upon ATP binding, it rapidly opens to allow the influx of small cations Na(+) and Ca(2+), and the K(+) efflux. Also has the ability to form a large pore in the cell membrane, allowing the passage of large cationic molecules. In microglia, may mediate NADPH transport across the plasma membrane. In immune cells, P2RX7 acts as a molecular sensor in pathological inflammatory states by detecting and responding to high local concentrations of extracellar ATP. In microglial cells, P2RX7 activation leads to the release of pro-inflammatory cytokines, such as IL-1beta and IL-18, through the activation of the NLRP3 inflammasome and caspase-1. Cooperates with KCNK6 to activate NLRP3 inflammasome. Activates death pathways leading to apoptosis and autophagy. Activates death pathways leading to pyroptosis; Shows ion channel activity but no macropore function; Non-functional channel; Non-functional channel[1][2][3][4][5][6][7][8][9][10][11].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Widely expressed with highest levels in brain and immune tissues; Predominant form in many tissues

  • Isoforms & Post-Translational Modification

    Q99572 has 9 isomers: Q99572-1: 68585 Da (predicted); Q99572-2: 41792 Da (predicted); Q99572-3: 14749 Da (predicted); Q99572-4: 49165 Da (predicted); Q99572-5: 31294 Da (predicted); Q99572-6: 35544 Da (predicted); Q99572-7: 31446 Da (predicted); Q99572-8: 58239 Da (predicted); Q99572-9: 29338 Da (predicted).
    Phosphorylation results in its inactivation;ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel;Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface (PubMed:18971257). Palmitoylation prevents channel desensitization by physically anchoring the palmitoylated groups to the membrane (By similarity)

  • Subunit

    Homotrimers (PubMed:20453110). Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230 and PTPRB (PubMed:11707406). Interacts (via C-terminus) with EMP2 (PubMed:12107182). Interacts with isoform B; this interaction potentiates P2RX7 responses (PubMed:20453110)

  • SwissProt ID

    Q99572

  • Gene ID
  • Synonyms

    P2X7

P2RX7 Antibody (YA4449) Related Classifications

MOQ
Minimum order quantity
100 mg

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