Ephrin A1 Antibody (YA9686)
(Synonyms: B61, ECKLG, EFL 1, EFL1, EFNA 1, Efna1, EFNA1_HUMAN, EPH related receptor tyrosine kinase ligand 1, EPH-related receptor tyrosine kinase ligand 1, Ephrin-A1)Ephrin A1 Antibody (YA9686) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to Ephrin A1.
-
Host:
Mouse
-
Isotype:
IgG
-
Application:
WB, ICC/IF, IHC-P
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100 | 1:500 |
Product Details
Ephrin A1 Antibody (YA9686) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to Ephrin A1.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman, Mouse, Rat
-
Calculated Molecular Weight Predicted band size: 24 kDa;
Recombinant protein of Human Ephrin A1 (aa 13-190).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Ephrin A1 is a Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Involved in angiogenesis, regulating vascular endothelial cell differentiation and migration through activation of EPHA2. Acts as a ligand for EPHA3, inhibiting epithelial-to-mesenchymal transition of cardiac cells and playing a role in heart development. May also contribute to dendritic spine morphogenesis (By similarity)[1][2][3].
-
Subcellular Localization
Cell membrane
-
Expression
Tissue_Specificity: Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
Induction: By TNF and IL1B/interleukin-1 beta. -
Isoforms & Post-Translational Modification
Ephrin A1 has 2 isoforms, P20827-1: amino acid length is 205, molecular weight is 23787 Da (predicted); P20827-2: amino acid length is 183, molecular weight is 21246 Da (predicted).Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form
-
Subunit
Monomer.
-
SwissProt ID
-
Synonyms
B61, ECKLG, EFL 1, EFL1, EFNA 1, Efna1, EFNA1_HUMAN, EPH related receptor tyrosine kinase ligand 1, EPH-related receptor tyrosine kinase ligand 1, Ephrin-A1
Documentation
References
[1]. Liu DP, et al. Ephrin-A1 is a negative regulator in glioma through down-regulation of EphA2 and FAK. Int J Oncol. 2007 Apr;30(4):865-71. [Content Brief]
[2]. Wykosky J, et al. Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor. Oncogene. 2008 Dec 11;27(58):7260-73. [Content Brief]
[3]. Sharfe N, et al. Ephrin-A1 induces c-Cbl phosphorylation and EphA receptor down-regulation in T cells. J Immunol. 2003 Jun 15;170(12):6024-32. [Content Brief]
Powered by Bioz
