TAF1 Antibody (YA2956)
(Synonyms: BA2R; CCG1; CCGS; DYT3; N TAF1; NSCL2; p250; TAF1; TAF2A; TAFII250; XDP)TAF1 Antibody (YA2956) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TAF1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
TAF1 Antibody (YA2956) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TAF1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 300 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 213 kDa
Entrez Gene: 8148 Human ; 70439 Mouse ; 287571 Rat
SwissProt: P21675 Human ; Q80UV9 Mouse ;
OMIM: 314250 Human
A synthesized peptide derived from human TAF1 1000-1100.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
TAF1 is a The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly. TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Nucleus
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Isoforms & Post-Translational Modification
P21675 has 15 isomers: P21675-2: 214714 Da (predicted); P21675-1: 212677 Da (predicted); P21675-13: 210736 Da (predicted); P21675-14: 212772 Da (predicted); P21675-15: 206664 Da (predicted); P21675-16: 210400 Da (predicted); P21675-17: 214913 Da (predicted); P21675-18: 210729 Da (predicted); P21675-19: 206955 Da (predicted); P21675-20: 207154 Da (predicted); P21675-21: 184091 Da (predicted); P21675-22: 210928 Da (predicted); P21675-23: 176469 Da (predicted); P21675-24: 177684 Da (predicted); P21675-25: 218488 Da (predicted).
Phosphorylated by casein kinase II in vitro -
Subunit
Component of the TFIID basal transcription factor complex, composed of TATA-box-binding protein TBP, and a number of TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:33795473, PubMed:7680771). Interacts with TAF7; the interaction is direct (PubMed:11592977, PubMed:25412659). TAF1, when part of the TFIID complex, interacts with C-terminus of TP53 (PubMed:15053879). Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:27007846). Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BACC1, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10 (PubMed:15960975). RB1 interacts with the N-terminal domain of TAF1 (PubMed:9858607). Interacts with ASF1A and ASF1B (PubMed:10759893, PubMed:12093919, PubMed:12842904). Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H1.4, H2A, H2B, H3 and H4 (in vitro) (PubMed:22464331)
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SwissProt ID
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Synonyms
BA2R; CCG1; CCGS; DYT3; N TAF1; NSCL2; p250; TAF1; TAF2A; TAFII250; XDP
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Research Field
Epigenetics and Nuclear Signaling
Documentation
[1]. Chen X, et al. Structural insights into preinitiation complex assembly on core promoters. Science. 2021 Apr 30;372(6541):. [Content Brief]
[2]. Wang H, et al. Crystal structure of a TAF1-TAF7 complex in human transcription factor IID reveals a promoter binding module. Cell Res. 2014 Dec;24(12):1433-44. [Content Brief]
[3]. Louder RK, et al. Structure of promoter-bound TFIID and model of human pre-initiation complex assembly. Nature. 2016 Mar 31;531(7596):604-9. [Content Brief]
[4]. Dikstein R, et al. TAFII250 is a bipartite protein kinase that phosphorylates the base transcription factor RAP74. Cell. 1996 Mar 8;84(5):781-90. [Content Brief]
[5]. Solow S, et al. Taf(II) 250 phosphorylates human transcription factor IIA on serine residues important for TBP binding and transcription activity. J Biol Chem. 2001 May 11;276(19):15886-92. [Content Brief]
[6]. Li HH, et al. Phosphorylation on Thr-55 by TAF1 mediates degradation of p53: a role for TAF1 in cell G1 progression. Mol Cell. 2004 Mar 26;13(6):867-78. [Content Brief]
[7]. Sekiguchi T, et al. The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. Mol Cell Biol. 1991 Jun;11(6):3317-25. [Content Brief]
[8]. Hisatake K, et al. The p250 subunit of native TATA box-binding factor TFIID is the cell-cycle regulatory protein CCG1. Nature. 1993 Mar 11;362(6416):179-81. [Content Brief]
[9]. O'Brien T, et al. Functional analysis of the human TAFII250 N-terminal kinase domain. Mol Cell. 1998 May;1(6):905-11. [Content Brief]
[10]. Siegert JL, et al. Rb inhibits the intrinsic kinase activity of TATA-binding protein-associated factor TAFII250. Mol Cell Biol. 1999 Jan;19(1):846-54. [Content Brief]
[11]. Hilton TL, et al. TAF1 histone acetyltransferase activity in Sp1 activation of the cyclin D1 promoter. Mol Cell Biol. 2005 May;25(10):4321-32. [Content Brief]
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